Results 281 to 290 of about 59,735 (325)
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In Vitro, 1981
partnership. Just as this new facility symbolizes the vigor of the ATCC, so this conference symbolizes the communal interest that must be accorded the preservation and distribution of microorganisms and other cell lines. The ATCC and other repositories are not simply means to facilitate the sharing of cells of contemporary interest.
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partnership. Just as this new facility symbolizes the vigor of the ATCC, so this conference symbolizes the communal interest that must be accorded the preservation and distribution of microorganisms and other cell lines. The ATCC and other repositories are not simply means to facilitate the sharing of cells of contemporary interest.
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Hybridoma autoantibodies to DNA.
The Journal of Immunology, 1980Abstract MRL/1 mice develop a severe form of systemic lupus erythematosus and spontaneously produce high levels of autoantibodies to DNA. Spleen cells from these animals were used as fusion partners to prepare hybridomas that produce autoantibodies to DNA.
Andrzejewski, C, Jr. +3 more
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1980
Kohler and Milstein (1975) launched a new era in immunological research by showing that somatic cell hybridization could be used to generate a continuous “hybridoma” cell line producing a monoclonal antibody. The hybridoma technology has already fulfilled one of the major goals that immunologists have been trying to achieve for years: the routine ...
Betty Diamond +3 more
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Kohler and Milstein (1975) launched a new era in immunological research by showing that somatic cell hybridization could be used to generate a continuous “hybridoma” cell line producing a monoclonal antibody. The hybridoma technology has already fulfilled one of the major goals that immunologists have been trying to achieve for years: the routine ...
Betty Diamond +3 more
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Secretion of Thymidine by Hybridoma Cells [PDF]
, 2006 Hybridoma cells secrete a factor (referred to in the following as ITI: inhibitor of radioactive thymidine incorporation) which inhibits incorporation of radioactive thymidine into cells 1. Of other cells tested, only myeloma cells were found to secrete ITI 2.
Dirk Petersen +3 more
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Cryopreservation of newly formed hybridomas
Journal of Immunological Methods, 1983A new freezing technique is described which permits time-consuming protocols as a first screening of newly formed hybridomas. In this procedure complete 96 well clustertrays with growing hybridomas are cryopreserved after programmed freezing. This procedure has been successfully applied to a number of fusion protocols for which the objective was to ...
DELEIJ, L, POPPEMA, S, THE, TH
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Creating hybridomas by electrofusion
Nature, 1988Electromanipulation has long been used to form pores in cell membranes to transfect them with foreign DNA. Now, the technique has been extended to the preparation of hybridomas.
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Hybridoma production and cloning with ORIGEN ? hybridoma cloning factor
Journal of Tissue Culture Methods, 1989Methods for producing and cloning B-cell hybridomas using ORIGEN ™ Hybridoma Cloning Factor as a replacement for feeder cells are described.
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Production of Antibodies in Hybridoma and Non-hybridoma Cell Lines [PDF]
, 2014 Antibody based drugs have not only advanced the prevention and treatment of a number of life threatening diseases including cancer and arthritis, but have also provided the thrust for the continued success of the biopharmaceutical and pharmaceutical industry.
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Process development for hybridoma cells
1988Revue des facteurs a considerer pour l'optimisation de la production d'anticorps monoclonaux a partir de lignees d ...
Harbour, C +2 more
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