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Cryopreservation of Hybridomas
2003Hybridomas are exposed to many threats, such as contamination with bacteria and fungi, loss of chromosomes coding for antibody production, overgrowth by nonsecreting mutants, and cell death resulting from overgrowth. Therefore, newly established hybridomas should be frozen and stored in liquid nitrogen at -196°C as soon as possible. Alternatively, they
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Hybridomas As a Source of Antibodies
Hospital Practice, 1981Hybridomas, the progeny of fusion between antibody-secreting spleen cells and myeloma cells, produce large quantities of a single species of antibody. They are an ideal source of serologic reagents since they can be grown in tissue culture, frozen, stored, and recovered when needed.
Pallaiah Thammana +2 more
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Cryopreservation of hybridomas
Journal of Tissue Culture Methods, 1985A method for the long-term storage of hybridoma cells in liquid nitrogen is described. Hybridoma cells are harvested in logarithmic phase of growth and suspensed in freeze medium containing dimethylsulfoxide. Using a programmable freezer, the cells are frozen very slowly (1°C/min) and then transferred rapidly to liquid nitrogen refrigerators ...
M. Irene Cour, Yvonne Reid
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2003
Hybrid clones will appear within 2-3 wk after fusion (see Chapter 46). It is of utmost importance that a newly established hybridoma is cloned thoroughly to ensure that the cells growing in the tissue culture are of monoclonal origin and not a mixture of two or more hybridomas.
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Hybrid clones will appear within 2-3 wk after fusion (see Chapter 46). It is of utmost importance that a newly established hybridoma is cloned thoroughly to ensure that the cells growing in the tissue culture are of monoclonal origin and not a mixture of two or more hybridomas.
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In Vitro, 1981
The National Institute of Allergy and Infectious Diseases is developing, with the American Type Culture Collection, a central facility for the acquisition, maintenance, and distribution of hybridoma cell lines. This effort finds its principal justification in the great activity in this field and in the large variety of hybridoma lines that are produced
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The National Institute of Allergy and Infectious Diseases is developing, with the American Type Culture Collection, a central facility for the acquisition, maintenance, and distribution of hybridoma cell lines. This effort finds its principal justification in the great activity in this field and in the large variety of hybridoma lines that are produced
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In Vitro, 1981
partnership. Just as this new facility symbolizes the vigor of the ATCC, so this conference symbolizes the communal interest that must be accorded the preservation and distribution of microorganisms and other cell lines. The ATCC and other repositories are not simply means to facilitate the sharing of cells of contemporary interest.
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partnership. Just as this new facility symbolizes the vigor of the ATCC, so this conference symbolizes the communal interest that must be accorded the preservation and distribution of microorganisms and other cell lines. The ATCC and other repositories are not simply means to facilitate the sharing of cells of contemporary interest.
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Stable antibody-producing murine hybridomas.
Science, 1983A method is described for obtaining antibody-producing hybridomas that are preferentially retained in cultures of fused mouse spleen and myeloma cells.
R. Taggart, I. Samloff
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Hybridoma autoantibodies to DNA.
The Journal of Immunology, 1980Abstract MRL/1 mice develop a severe form of systemic lupus erythematosus and spontaneously produce high levels of autoantibodies to DNA. Spleen cells from these animals were used as fusion partners to prepare hybridomas that produce autoantibodies to DNA.
Andrzejewski, C, Jr. +3 more
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1980
Kohler and Milstein (1975) launched a new era in immunological research by showing that somatic cell hybridization could be used to generate a continuous “hybridoma” cell line producing a monoclonal antibody. The hybridoma technology has already fulfilled one of the major goals that immunologists have been trying to achieve for years: the routine ...
Betty Diamond +3 more
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Kohler and Milstein (1975) launched a new era in immunological research by showing that somatic cell hybridization could be used to generate a continuous “hybridoma” cell line producing a monoclonal antibody. The hybridoma technology has already fulfilled one of the major goals that immunologists have been trying to achieve for years: the routine ...
Betty Diamond +3 more
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Secretion of Thymidine by Hybridoma Cells [PDF]
, 2006 Hybridoma cells secrete a factor (referred to in the following as ITI: inhibitor of radioactive thymidine incorporation) which inhibits incorporation of radioactive thymidine into cells 1. Of other cells tested, only myeloma cells were found to secrete ITI 2.
Dirk Petersen +3 more
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