Results 61 to 70 of about 792,258 (371)
Functional approach to the catalytic site of the sarcoplasmic reticulum Ca(2+)-ATPase: binding and hydrolysis of ATP in the absence of Ca(2+) [PDF]
J Bioenerg Biomembr . 2004 Jun;36(3):265-73Isolated sarcoplasmic reticulum vesicles in the presence of Mg(2+) and absence of Ca(2+) retain significant ATP hydrolytic activity that can be attributed to the Ca(2+)-ATPase protein. At neutral pH and the presence of 5 mM Mg(2+), the dependence of the hydrolysis rate on a linear ATP concentration scale can be fitted by a single hyperbolic function ...
arxiv +1 more source
Mapping Hsp104 interactions using cross‐linking mass spectrometry
FEBS Open Bio, EarlyView.This study examines how cross‐linking mass spectrometry can be utilized to analyze ATP‐induced conformational changes in Hsp104 and its interactions with substrates. We developed an analytical pipeline to distinguish between intra‐ and inter‐subunit contacts within the hexameric homo‐oligomer and discovered contacts between Hsp104 and a selected ...
Kinga Westphal +3 more
wiley +1 more source
Utilization of ascites plasma very low density lipoprotein triglycerides by Ehrlich cells
Journal of Lipid Research, 1974 Much of the lipid present in the ascites plasma in which Ehrlich cells grow is contained in very low density lipoproteins (VLDL). Chemical measurements indicated that triglycerides were taken up by the cells during in vitro incubation with ascites VLDL ...
Douglas E. Brenneman, Arthur A. Spector
doaj
Structure, hydrolysis and diffusion of aqueous vanadium ions from Car-Parrinello molecular dynamics [PDF]
The Journal of Chemical Physics 145, 000000 (2016), 2016 A molecular level understanding of the properties of electroactive vanadium species in aqueous solution is crucial for enhancing the performance of vanadium redox flow batteries (RFB). Here, we employ Car-Parrinello molecular dynamics (CPMD) simulations based on density functional theory to investigate the hydration structures, first hydrolysis ...
arxiv +1 more source
Comparative study of adenosine 3′‐pyrophosphokinase domains of MuF polymorphic toxins
FEBS Open Bio, EarlyView.With the ultimate goal of understanding the association of toxin‐immunity modules to temperate phages, we characterized toxins from three prophages and examined cross‐protection from immunity proteins. The toxins exhibit adenosine 3′‐pyrophosphokinase activity and are toxic in Escherichia coli.
Eloïse M. Paulet +6 more
wiley +1 more source
Analysis of fatty acids of human red cells without lipid extraction
Journal of Lipid Research, 1968 Exposure of human red cells to 2 n HCl for 18-20 hr at 110°C appears to release the total fatty acid, which can then be esterified for GLC analysis. This technique is simpler and may be more reliable than the conventional methods that depend on lipid ...
Gerald B. Phillips +2 more
doaj
Dyna, 2018 The use of more biocompatible and renewable chemical compounds to obtain metal nanoparticles with desired properties and characteristics becomes an alternative route for the reduction of environmental risks and to minimize the incompatibility of these ...
Wilson Agudelo +2 more
doaj +1 more source
Advanced Biology, EarlyView.Coacervation driven by liquid‐liquid phase separation (LLPS) of biopolymers has garnered increasing attention in biology since this leads to the formation of membraneless organelles capable of performing essential yet largely unknown functions. This review highlights recent advances in coacervates (artificial condensates) composed of low‐molecular ...
Sayuri L. Higashi, Masato Ikeda
wiley +1 more source
Brazilian Archives of Biology and Technology, 2011 In this work, an α- amylase producing Fusarium sp. was isolated from the soil at 50 0C. Growth and enzyme production occurred at 30, 45 and 55 ºC.
Tochukwu Nwamaka Nwagu +1 more
doaj +1 more source
Foods and Raw Materials, 2014 Protein hydrolysates have a high biological and nutritional value and are widely used in various sectors of the food, medical, and pharmaceutical industries. This article deals with the chemical hydrolysis of the milk protein casein in the presence of hydrochloric or sulfuric acid and reports the hydrolysis parameters minimizing the loss of amino acids.
Kurbanova, Marina +1 more
openaire +5 more sources