Results 181 to 190 of about 13,983 (221)
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Purification of hypoxanthine-guanine phosphoribosyltransferase of Plasmodium lophurae
Molecular and Biochemical Parasitology, 1987Hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8) was isolated from the malarial parasite, Plasmodium lophurae. The apparent pI, as determined by chromatofocusing, was 7.6. The native molecular weight was 79,000. The pH profile of HGPRT exhibited a broad pH optimum. With hypoxanthine as substrate maximal activity was achieved from pH 6.0-10.0,
C M, Schimandle, L A, Mole, I W, Sherman
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Rapid detection of hypoxanthine-guanine phosphoribosyltransferase on cellogel
Humangenetik, 1974A simple, fast and direct staining method for the detection of hypoxanthineguanine phosphoribosyltransferase is described. It is based on the conversion of inosine monophosphate to hypoxanthine, which is then enzymatically oxidized. This oxidation is coupled to the reduction of a tetrazolium salt to blue formazan.
Someren, H. van +2 more
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Hypoxanthine-guanine phosphoribosyltransferase from beef brain: A trimer
Biochemical and Biophysical Research Communications, 1980Abstract Hypoxanthine-guanine phosphoribosyl isolated from beef brain was reacted with cross-linking reagents in order to establish the number of subunits that constitute the native protein. The results obtained from experiments with dimethyl-suberimidate and gluteraldehyde in the absence and in the presence of substrates all indicate that the native
V A, Paulus, A L, Bieber
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A continuous spectrophotometric assay for hypoxanthine-guanine phosphoribosyltransferase
Analytical Biochemistry, 1977Abstract The present paper describes a simple and rapid spectrophotometric assay of hypoxanthine-guanine phosphoribosyltransferase based on the continuous monitoring of product concentration by a NADH-coupled enzyme system.
GIACOMELLO, Alessandro +1 more
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A rapid radiochemical assay for hypoxanthine-guanine phosphoribosyltransferase
Analytical Biochemistry, 1973Abstract A simple radiochemical method is described for assay of hypoxanthine-guanine phosphoribosyltransferase. 14C-Hypoxanthine is incubated with enzyme PRPP. The labelled product is precipitated on strips of Whatman No. 1 paper by the addition of lanthanum nitrate. Unreacted substrate is eluted with distilled water.
M A, Schlossberg, V P, Hollander
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[Deficiencies of hypoxanthine guanine phosphoribosyltransferase (HPRT)].
Nihon rinsho. Japanese journal of clinical medicine, 2008Inherited mutations of a purine salvage enzyme, hypoxanthine guanine phosphoribosyltransferase (HPRT, EC 2.4.2.8; MIM308000), give rise to Lesch-Nyhan syndrome (MIM300322) or HPRT-related gout called as Kelley-Seegmiller syndrome (MIM300323). In contrast with the most severe phenotype of classical Lesch-Nyhan disease (LND), the least severe phenotype ...
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Human Hypoxanthine-Guanine Phosphoribosyltransferase (HGPRT): Purification and Properties
1974Hypoxanthine-guanine phosphoribosyltransferase (HGPRT) (E.C. 2.4.2.8.) catalyzes the formation of guanosine-5’-monophosphate and inosine-5’-monophosphate from 5’-phosphoribosyl-l-pyrophosphate (PPribose-P) and the purine bases guanine and hypoxanthine, respectively.
W J, Arnold, R V, Lamb, W N, Kelley
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The Spectrum of Hypoxanthine-Guanine Phosphoribosyltransferase (HPRT) Deficiency
2006Summary The enzyme hypoxanthine-guanine phosphoribo-syltransferase (HPRT) catalyzes the reutilization ofhypoxanthine and guanine to the purine nucleotidesIMP and GMP, respectively. HPRT deficiency is an X-linked disorder characterized by uric acid over-production and variable neurologic impairment.
J G, Puig +5 more
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Hypoxanthine Guanine Phosphoribosyltransferase (HPRT) Mutations in the Asian Population
Nucleosides, Nucleotides and Nucleic Acids, 2011Mutation of hypoxanthine guanine phosphoribosyltransferase (HPRT) gives rise to Lesch-Nyhan syndrome, which is characterized by hyperuricemia, severe motor disability, and self-injurious behavior, or HPRT-related gout (Kelley-Seegmiller syndrome). The marked heterogeneity of HPRT deficiency is well known, with more than 300 mutations at the HPRT gene ...
Y, Yamada +4 more
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Hypoxanthine‐Guanine Phosphoribosyltransferase Activity in Normal and Leukaemic Lymphocytes
British Journal of Haematology, 1981Summary. The levels of hypoxanthine‐guanine phosphoribosyltransferase (HGPRT) were determined in lymphocytes from normal people and patients with chronic lymphocytic leukaemia (CLL). The HGPRT level in the total lymphocyte population from patients with CLL was lower than that from normal subjects.
P, Rambotti, S, Davis
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