Results 251 to 260 of about 51,551 (285)
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Hypoxanthine-Guanine Phosphoribosyltransferase Deficiency
New England Journal of Medicine, 1983A DEFICIENCY in the enzyme hypoxanthineguanine phosphoribosyltransferase (HPRT) is associated with two clinical syndromes.
Jeffrey S. Flier +4 more
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Hypoxanthine, Guanine, Xanthine Phosphoribosyltransferase Activity inCryptosporidium parvum
Experimental Parasitology, 1998All parasitic protozoa examined to date are incapable of de novo synthesis of purine nucleotides and rely on salvage mechanisms for survival. We have identified hypoxanthine, guanine, xanthine phosphoribosyl-transferase activities in crude cell-free extracts of Cryptosporidium sporulated oocysts utilizing radiolabeled substrates. Guanine, hypoxanthine,
P S, Doyle, J, Kanaani, C C, Wang
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Biochemical Medicine, 1973
Abstract Hypoxanthine transport has been studied in cultured human fibroblasts with normal and mutant H-G PRT. Transport is dependent on cell density, the activity of H-G PRT, and de novo purine synthesis. Transport is decreased in control cultures at high density and in cell strains with a mutant H-G PRT.
P J, Benke, N, Herrick, A, Herbert
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Abstract Hypoxanthine transport has been studied in cultured human fibroblasts with normal and mutant H-G PRT. Transport is dependent on cell density, the activity of H-G PRT, and de novo purine synthesis. Transport is decreased in control cultures at high density and in cell strains with a mutant H-G PRT.
P J, Benke, N, Herrick, A, Herbert
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Genetic Analysis of Human Hypoxanthine-Guanine Phosphoribosyltransferase Deficiency
Enzyme, 1987Hypoxanthine-guanine phosphoribosyltransferase (HPRT; IMP: pyrophosphate phosphoribosyltransferase, EC 2.4.2.8) functions in the purine-metabolic salvage pathway. Two clinical syndromes are associated with a deficiency in HPRT enzyme activity. Virtually complete deficiency leads to the Lesch-Nyhan syndrome, whereas partial deficiency results in ...
L J, Silverman +2 more
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Clinical correlations in partial hypoxanthine guanine phosphoribosyltransferase deficiency
Pediatric Neurology, 1986Erythrocyte assays for hypoxanthine guanine phosphoribosyltransferase (HGPRT) activity performed on two male half-siblings with hyperuricemia, produced results consistent with classic Lesch-Nyhan syndrome. Due to the absence of neurologic abnormalities, cognitive deficits, or self-mutilation, HGPRT activity was measured by intact fibroblast assay which
J H, Hersh +5 more
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Purification of hypoxanthine-guanine phosphoribosyltransferase of Plasmodium lophurae
Molecular and Biochemical Parasitology, 1987Hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8) was isolated from the malarial parasite, Plasmodium lophurae. The apparent pI, as determined by chromatofocusing, was 7.6. The native molecular weight was 79,000. The pH profile of HGPRT exhibited a broad pH optimum. With hypoxanthine as substrate maximal activity was achieved from pH 6.0-10.0,
C M, Schimandle, L A, Mole, I W, Sherman
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Rapid detection of hypoxanthine-guanine phosphoribosyltransferase on cellogel
Humangenetik, 1974A simple, fast and direct staining method for the detection of hypoxanthineguanine phosphoribosyltransferase is described. It is based on the conversion of inosine monophosphate to hypoxanthine, which is then enzymatically oxidized. This oxidation is coupled to the reduction of a tetrazolium salt to blue formazan.
Someren, H. van +2 more
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1974
Partial deficiency of HGPRT, a salvage enzyme of purine metabolism, has been demonstrated to be the primary abnormality causing purine overproduction in a small proportion of patients with gout (1–4). The quantitative deviation in the activity of this enzyme has been shown by Kelley et al.
O, Sperling, P, Boer, A, De Vries
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Partial deficiency of HGPRT, a salvage enzyme of purine metabolism, has been demonstrated to be the primary abnormality causing purine overproduction in a small proportion of patients with gout (1–4). The quantitative deviation in the activity of this enzyme has been shown by Kelley et al.
O, Sperling, P, Boer, A, De Vries
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Evidence for tetrameric structure of mammalian hypoxanthine phosphoribosyltransferase
Biochemical Genetics, 1987A fast electrophoretic variant of hypoxanthine phosphoribosyltransferase (HPRT) has been detected in Mus musculus bactrianus, a mouse subspecies from Middle Asia (USSR). The electrophoretic HPRT pattern yielded by hybrids between the somatic cell of LMTK- (deficient in thymidine kinase) and the splenocytes of a male of M. m. bactrianus was five-banded.
M N, Bochkarev +5 more
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Human Hypoxanthine-Guanine Phosphoribosyltransferase (HGPRT): Purification and Properties
1974Hypoxanthine-guanine phosphoribosyltransferase (HGPRT) (E.C. 2.4.2.8.) catalyzes the formation of guanosine-5’-monophosphate and inosine-5’-monophosphate from 5’-phosphoribosyl-l-pyrophosphate (PPribose-P) and the purine bases guanine and hypoxanthine, respectively.
W J, Arnold, R V, Lamb, W N, Kelley
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