Results 71 to 80 of about 357,551 (214)
Sea ice observations have been conducted onboard the Antarctic Research Vessel Shirase" since . The authors summarize these data to investigate spatial and inter-annual variability of sea ice thickness and snow depth of the summer land-fast ice in Lutzow-
Shotaro Uto +7 more
doaj +1 more source
Decline in plankton diversity and carbon flux with reduced sea ice extent along the Western Antarctic Peninsula. [PDF]
Lin Y +10 more
europepmc +1 more source
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
A twenty-first century structural change in Antarctica’s sea ice system
From 1979 to 2016, total Antarctic sea ice extent experienced a positive trend with record winter maxima in 2012 and 2014. Record summer minima followed within the period 2017-2024, raising the possibility that the Antarctic sea ice system might be ...
Marilyn N. Raphael +4 more
doaj +1 more source
Dual-Mode Sea Ice Extent Retrieval for the Rotating Fan Beam Scatterometer
Scatterometers are highlighted in polar applications, such as sea ice extent retrieval. There are currently three types of spaceborne scatterometer in operation, among which the rotating pencil beam scatterometer and the rotating fan beam scatterometer ...
Liling Liu +3 more
doaj +1 more source
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey +4 more
wiley +1 more source
The two catalytic subunits of typhoid toxin dissociate from the holotoxin in the ER of an intoxicated cell, but only CdtB exits the ER to generate immunosuppressive effects. PltA is retained in the ER and sequestered from its cytosolic target, thus allowing the anti‐inflammatory effects of CdtB to promote intestinal colonization.
Maria C. Zabala‐Rodriguez +4 more
wiley +1 more source
Cell surface CD11c as a neutrophil aging marker molecule
Cell surface CD11chi neutrophils were more aged and had better phagocytic function than CD11c−/lo neutrophils. Transcriptomic analysis of CD11chi neutrophils and CD11c−/lo neutrophils in pediatric population showed that the most difference was seen in infants.
Sophia Koutsogiannaki +5 more
wiley +1 more source
The C‐terminal domain of yeast Arginyltransferase1 is essential for its catalytic activity
Arginyltransferase 1 (Ate1), a eukaryotic enzyme, catalyses arginylation, transferring arginine from tRNA‐Arg to the amino terminus of the target protein. Overexpression of Ate1 in yeast is lethal and is dependent on arginylation. This study elucidates how mutations in the cofactor‐binding and active site of Ate1 and truncation of its structural ...
Vikas Kumar Yadav +4 more
wiley +1 more source

