Results 51 to 60 of about 59,874 (263)

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

open access: yesFEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata   +13 more
wiley   +1 more source

Velocity increases at Cook Glacier, East Antarctica, linked to ice shelf loss and a subglacial flood event [PDF]

open access: yesThe Cryosphere, 2018
Cook Glacier drains a large proportion of the Wilkes Subglacial Basin in East Antarctica, a region thought to be vulnerable to marine ice sheet instability and with potential to make a significant contribution to sea level.
B. W. J. Miles   +2 more
doaj   +1 more source

Dose‐dependent hepatotoxicity of hydrogen peroxide in HepG2 cells and its modulation by CYP450 induction

open access: yesFEBS Open Bio, EarlyView.
NMR metabolomics revealed concentration‐dependent metabolic perturbations in HepG2 cells exposed to H2O2. Rifampicin pretreatment enhanced metabolic competence, attenuated toxin‐induced alterations and produced metabolite profiles more consistent with human liver physiology, supporting the use of CYP450‐induced HepG2 models for improved in vitro ...
Maren Jinks   +4 more
wiley   +1 more source

balance velocities of the Greenland Ice Sheet [PDF]

open access: yesGeophysical Research Letters, 1997
We present a map of balance velocities for the Greenland ice sheet. The resolution of the underlying DEM, which was derived primarily from radar altimetery data, yields far greater detail than earlier balance velocity estimates for Greenland. The velocity contours reveal in striking detail the location of an ice stream in northeastern Greenland, which ...
Ian Joughin   +3 more
openaire   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

open access: yesFEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty   +13 more
wiley   +1 more source

Local scour around a bridge pier under ice-jammed flow condition – an experimental study

open access: yesJournal of Hydrology and Hydromechanics, 2021
The appearance of an ice jam in a river crucially distorts local hydrodynamic conditions including water level, flow velocity, riverbed form and local scour processes.
Wang Jun   +5 more
doaj   +1 more source

Velocity Anomaly of Campbell Glacier, East Antarctica, Observed by Double-Differential Interferometric SAR and Ice Penetrating Radar

open access: yesRemote Sensing, 2021
Regional changes in the flow velocity of Antarctic glaciers can affect the ice sheet mass balance and formation of surface crevasses. The velocity anomaly of a glacier can be detected using the Double-Differential Interferometric Synthetic Aperture Radar
Hoonyol Lee   +4 more
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

Decadal GPS-derived ice surface velocity along the transect from Zhongshan Station to and around Dome Argus, East Antarctica, 2005–16

open access: yesAnnals of Glaciology, 2018
Using repeat GPS measurements during 2005–16, we calculated and updated two-dimensional high-resolution decadal ice surface velocity estimates along the traverse route from Zhongshan Station to and around Dome Argus, East Antarctica.
Yang Yuande   +8 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

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