Results 101 to 110 of about 984 (123)

Antibiotic discovery: combining isolation chip (iChip) technology and co-culture technique

Applied Microbiology and Biotechnology, 2018
Antibiotics had been a useful tool for treating bacterial infections since their discovery, but with the passage of time, the evolution of resistance among microbes against antibiotics has rendered them useless. Many approaches are being used to tackle this problem which include discovery of new antibiotics, modification of the existing ones, and ...
Yulin Deng, Deng Yulin
exaly   +3 more sources

Efficient isolation of specific genomic regions retaining molecular interactions by the iChIP system using recombinant exogenous DNA-binding proteins [PDF]

BMC Molecular Biology, 2014
Abstract Background Comprehensive understanding of mechanisms of genome functions requires identification of molecules interacting with genomic regions of interest in vivo .
Toshitsugu Fujita, Hodaka Fujii, Fujita Toshitsugu   +2 more
exaly   +5 more sources

Efficient isolation of specific genomic regions by insertional chromatin immunoprecipitation (iChIP) with a second-generation tagged LexA DNA-binding domain

Advances in Bioscience and Biotechnology (Print), 2012
Comprehensive understanding of mechanisms of epigenetic regulation requires identification of molecules bound to genomic regions of interest in vivo. We have developed a novel method, insertional chromatin immunoprecipitatin (iChIP), to isolate specific genomic regions retaining molecular interaction in order to perform non-biased identification of ...
Toshitsugu Fujita, Hodaka Fujii
exaly   +3 more sources

UTILIZING THE DHS REACTOR AND ICHIP FOR CULTIVATING POTENT UREASE-PRODUCING BACTERIA FOR BIOCEMENTATION

土木学会論文集
Masashi Hatamoto
exaly   +3 more sources

In situ cultivation of previously uncultivable microorganisms using the ichip

Nature Protocols, 2017
Most microbial species remain uncultivated, and modifying artificial nutrient media brings only an incremental increase in cultivability. We reasoned that an alternative way to cultivate species with unknown requirements is to use naturally occurring combinations of growth factors.
Brittany, Berdy, Amy L, Spoering, Losee L, Ling, Slava S, Epstein   +3 more
openaire   +2 more sources

Teixobactin: Antibiotikum vom iChip

Chemie in unserer Zeit, 2015
AbstractDie meisten gegenwärtig verwendeten Antibiotika sind Naturstoffe oder Naturstoffderivate. Der wichtigste Ansatz, neue Antibiotika zu entdecken, ist daher das Screening von natürlichen Quellen. Einem amerikanisch‐deutsch‐englischen Forschungszusammenschluss ist es jüngst gelungen, ein neues Antibiotikum zu extrahieren und zu charakterisieren ...
openaire   +1 more source

Isolation of Soil Microorganisms Using iChip Technology

Journal of Visualized Experiments
The iChip isolation technique uses an in-situ isolation device that increases the cultivability of previously unculturable microorganisms. Microorganisms are an important source of novel chemistries and potentially bioactive molecules. However, only 1% of environmental microorganisms can be cultured using conventional laboratory methods.
Leah K, Gauthier, Adam, Foster, Brian D, Wagner, Christopher W, Kirby   +3 more
openaire   +2 more sources

An Ichip-Domesticated Sponge Bacterium Produces an N-Acyltyrosine Bearing an α-Methyl Substituent

Organic Letters, 2019
The ichip (isolation chip) was employed for the first time in a marine sponge (Xestospongia muta), and a putatively new bacterial species, Alteromonas sp. RKMC-009, was isolated. Strain RKMC-009 produces a novel N-acyltyrosine (1) that is appended with a rare α-methyl substituent within the aminoacyl moiety and also exhibits Gram-positive antibacterial
Logan W. MacIntyre, Marie J. Charles, Bradley A. Haltli, Douglas H. Marchbank, Russell G. Kerr   +4 more
openaire   +2 more sources

Three New Diketopiperazines from the Previously Uncultivable Marine Bacterium Gallaecimonas mangrovi HK‐28 Cultivated by iChip

Chemistry & Biodiversity, 2020
AbstractThe in situ application of iChip cultivation in mangrove sediment from Hainan province, China, led to the isolation of a novel bacterial species Gallaecimonas mangrovi HK‐28. The extract of G. mangrovi HK‐28 exhibited antibiotic activity against the aquatic pathogen Vibrio harveyi, and its chemical constituents were further investigated by ...
Lijian Ding, Peng Xu, Weiyan Zhang, Ye Yuan, Xiaoping He, Dengquan Su, Yutong Shi, C. Benjamin Naman, Xiaojun Yan, Bin Wu, J. Enrico H. Lazaro, Shengying Li, Shan He   +12 more
openaire   +2 more sources

Use of a modified iChip for bacterial isolation from a tropical peat swamp forest with emphasis on a novel antimicrobial-producing bacterium Chitinophaga extrema

2021
The present study looks into 3D-printing an isolation chip device that improves the cultivation and recovery of previously unculturable environmental microorganisms from a pristine tropical peat forest. The 3D-printed device has led to the isolation of a pool of microorganisms with considerable diversity and novelty compared to standard cultivation ...
openaire   +1 more source