Results 131 to 140 of about 1,073 (151)

i C h IP ‐ SILAC analysis identifies epigenetic regulators of C p G methylation of the p16 INK4A gene

FEBS Letters
iChIP‐SILAC identified DNMT1 and epigenetic regulators as proteins potentially interacting with a region of the p16INK4A gene that is CpG‐methylated in one allele in HCT116 cells. Among the identified proteins, DDX24 contributed to the CpG methylation by regulating the protein level of DNMT1.
Toshitsugu Fujita, Hodaka Fujii
exaly   +4 more sources

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FACS-iChip: a high-efficiency iChip system for microbial ‘dark matter’ mining

Marine Life Science and Technology, 2020
The isolation chip method (iChip) provides a novel approach for culturing previously uncultivable microorganisms; this method is currently limited by the user being unable to ensure single-cell loading within individual wells. To address this limitation, we integrated flow cytometry-based fluorescence-activated cell sorting with a modified iChip (FACS ...
Ran Xue, Yiling Wang, Erinne Stirling
exaly   +3 more sources

Negative-Selection Enrichment of Circulating Tumor Cells from Peripheral Blood Using the Microfluidic CTC-iChip

Methods in Molecular Biology, 2022
The ability to isolate and analyze rare circulating tumor cells (CTCs) holds the potential to increase our understanding of cancer evolution and allows monitoring of disease and therapeutic responses through a relatively non-invasive blood-based biopsy.
Risa Burr, Jon F, Brian Chirn
exaly   +3 more sources

Antibiotic discovery: combining isolation chip (iChip) technology and co-culture technique

Applied Microbiology and Biotechnology, 2018
Antibiotics had been a useful tool for treating bacterial infections since their discovery, but with the passage of time, the evolution of resistance among microbes against antibiotics has rendered them useless. Many approaches are being used to tackle this problem which include discovery of new antibiotics, modification of the existing ones, and ...
Yulin Deng, Deng Yulin
exaly   +3 more sources

Efficient isolation of specific genomic regions retaining molecular interactions by the iChIP system using recombinant exogenous DNA-binding proteins [PDF]

BMC Molecular Biology, 2014
Abstract Background Comprehensive understanding of mechanisms of genome functions requires identification of molecules interacting with genomic regions of interest in vivo .
Toshitsugu Fujita, Hodaka Fujii, Fujita Toshitsugu   +2 more
exaly   +5 more sources

Efficient isolation of specific genomic regions by insertional chromatin immunoprecipitation (iChIP) with a second-generation tagged LexA DNA-binding domain

Advances in Bioscience and Biotechnology (Print), 2012
Comprehensive understanding of mechanisms of epigenetic regulation requires identification of molecules bound to genomic regions of interest in vivo. We have developed a novel method, insertional chromatin immunoprecipitatin (iChIP), to isolate specific genomic regions retaining molecular interaction in order to perform non-biased identification of ...
Toshitsugu Fujita, Hodaka Fujii
exaly   +3 more sources

UTILIZING THE DHS REACTOR AND ICHIP FOR CULTIVATING POTENT UREASE-PRODUCING BACTERIA FOR BIOCEMENTATION

土木学会論文集
Masashi Hatamoto, Takashi Yamaguchi
exaly   +3 more sources

Isolation of Soil Microorganisms Using iChip Technology

Journal of Visualized Experiments
The iChip isolation technique uses an in-situ isolation device that increases the cultivability of previously unculturable microorganisms. Microorganisms are an important source of novel chemistries and potentially bioactive molecules. However, only 1% of environmental microorganisms can be cultured using conventional laboratory methods.
Leah K, Gauthier, Adam, Foster, Brian D, Wagner, Christopher W, Kirby   +3 more
openaire   +2 more sources

Teixobactin: Antibiotikum vom iChip

Chemie in unserer Zeit, 2015
AbstractDie meisten gegenwärtig verwendeten Antibiotika sind Naturstoffe oder Naturstoffderivate. Der wichtigste Ansatz, neue Antibiotika zu entdecken, ist daher das Screening von natürlichen Quellen. Einem amerikanisch‐deutsch‐englischen Forschungszusammenschluss ist es jüngst gelungen, ein neues Antibiotikum zu extrahieren und zu charakterisieren ...
openaire   +1 more source

Use of a modified iChip for bacterial isolation from a tropical peat swamp forest with emphasis on a novel antimicrobial-producing bacterium Chitinophaga extrema

2021
The present study looks into 3D-printing an isolation chip device that improves the cultivation and recovery of previously unculturable environmental microorganisms from a pristine tropical peat forest. The 3D-printed device has led to the isolation of a pool of microorganisms with considerable diversity and novelty compared to standard cultivation ...
openaire   +1 more source