Microgels With Electrostatically Controlled Molecular Affinity to Direct Morphogenesis
This work presents a precision toolbox to modulate morphogen gradients in tissue cultures. Spatial and temporal gradient control is achieved using sulfated glycosaminoglycan‐based microgels (µGUIDEs) with electrostatically tunable morphogen affinity for tailored release. Individual µGUIDEs act as artificial signaling centers, locally directing vascular
Sebastian Kühn +10 more
wiley +1 more source
Effect of PCSK9 natural mutants on TGN localization and trafficking dynamics by FRAP and iFRAP.
(A) HepG2 cells were transfected with PCSK9-mC WT, GOF S127R, D129G, D374Y or LOF R46L mutants (red) and TGN localization was determined by colocalization (arrows) with Golgin-97 (green) and analysis by confocal microscopy.
Annie Demers (369643) +4 more
core +1 more source
Additional file 1: Figure. S1 Characterization of conditional KO MEFs. a-c. Immunoblot analyses of whole cell extracts of WT and Stag2 KO (a), Pds5 DKO (b) and Ctcf KO (c) MEFs used for ChIP analyses.
Daniel Giménez-Llorente (14176863) +6 more
core +1 more source
Dynamic and lifetime analysis of the GlCHC:GFP reporter.
(A-D) FRAP and iFRAP analyses were done in the same field of view including an unbleached control cell (ROI05). FRAP analysis (ROI02, ROI03) shows no recovery whilst iFRAP analysis (ROI04) shows no loss of fluorescence after 225s, indicating no ...
Adrian B. Hehl (74036) +5 more
core +1 more source
Advanced Fluorescence Microscopy Techniques-FRAP, FLIP, FLAP, FRET and FLIM [PDF]
Fluorescence microscopy provides an efficient and unique approach to study fixed and living cells because of its versatility, specificity, and high sensitivity.
Ankerhold, Richard +5 more
core +1 more source
AJs exhibit lateral diffusion and internalization independent of contact elongation.
(A) iFRAP of mid stage AS cells expressing Arm-GFP. Square brackets indicate bleached regions. Kymograph of black boxed region shows behavior of unbleached line over time. Blue dots track contact length change.
Tony J. C. Harris (251011) +1 more
core +1 more source
Étude du trafic cellulaire de la convertase de proprotéine PCSK9 responsable de la dégradation du récepteur des lipoprotéines de faible densité (LDLR) [PDF]
Les maladies cardiovasculaires (MCV) sont la principale cause de mortalité dans les pays industrialisés. L'hypercholestérolémie constitue un facteur de risque majeur pour les MCV.
Ait Hamouda, Hocine
core +2 more sources
The Diffusion Model of Intra-Golgi Transport Has Limited Power. [PDF]
Beznoussenko GV +5 more
europepmc +1 more source
Acceptability of, and preferences for, remote consulting during COVID-19 among older patients with two common long-term musculoskeletal conditions: findings from three qualitative studies and recommendations for practice. [PDF]
Paskins Z +11 more
europepmc +1 more source
Revue de presse lundi 7 avril 2014
• Transition énergétique : arrêtons les objectifs irrationnels / Philippe François et Agnès Verdier-Molinié, Fondation iFRAP, Les Echos, 7/04/2014 http://www.lesechos.fr/opinions/points_vue/0203402583539-transition-energetique-arretons-les-objectifs ...
Danièle Revel
core +1 more source

