Results 301 to 310 of about 285,255 (319)
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Ruthenium counterstaining for imaging mass cytometry
The Journal of Pathology, 2018AbstractImaging mass cytometry is a novel imaging modality that enables simultaneous antibody‐based detection of >40 epitopes and molecules in tissue sections at subcellular resolution by the use of isotopically pure metal tags. Essential for any imaging approach in which antigen detection is performed is counterstaining, which reveals the overall ...
Catena, Raúl +2 more
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Role of DNA flow cytometry and image cytometry on effusion fluid
Diagnostic Cytopathology, 2000The objective of the study was to assess the value of DNA flow cytometry (FCM) and image cytometry (ICM) as an adjunct to routine diagnostic cytology. In this prospective study, 100 consecutive effusion fluids were studied for routine cytology, DNA FCM, and in selected cases, ICM.
Harpreet Vhora +3 more
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AOTF-based system for image cytometry
SPIE Proceedings, 2005This paper describes a simple, inexpensive multispectral im aging system for image cytometry applications. The system is based on an acousto-optical tunable filter (AOTF), a monochrome CCD camera, and a research-grade fluorescence microscope. The selected TeO2 AOTF has a 10x10 mm 2 entrance aperture and operates within the spectral range of 447–750 nm.
Rajwa, Bartek +7 more
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Combined flow cytometry and image cytometry of the same cytological sample
Journal of Microscopy, 1983SUMMARYFlow cytometry and image cytometry, two measuring techniques in the field of analytical cytology, can be used sequentially on the same cytological sample. Cells stained with a fluorochrome for the determination of for example, DNA or RNA content are first analysed in suspension by flow cytometry.
Hans J. Tanke +3 more
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Competence on demand in DNA image cytometry
Pathology - Research and Practice, 2000Quantitation methods in clinical pathology have to be normalized and standardized both from the instrumental and the methodological point of view to guarantee a defined level of precision and accuracy independent of the site where they are applied.
Gunter Haroske +4 more
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2016
Imaging flow cytometry (IFC) platforms combine features of flow cytometry and fluorescent microscopy with advances in data-processing algorithms. IFC allows multiparametric fluorescent and morphological analysis of thousands of cellular events and has the unique capability of identifying collected events by their real images. IFC allows the analysis of
Ivan A. Vorobjev +2 more
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Imaging flow cytometry (IFC) platforms combine features of flow cytometry and fluorescent microscopy with advances in data-processing algorithms. IFC allows multiparametric fluorescent and morphological analysis of thousands of cellular events and has the unique capability of identifying collected events by their real images. IFC allows the analysis of
Ivan A. Vorobjev +2 more
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Imaging flow cytometry for phytoplankton analysis
Methods, 2017This review highlights the concepts and instrumentation of imaging flow cytometry technology and in particular its use for phytoplankton analysis. Imaging flow cytometry, a hybrid technology combining speed and statistical capabilities of flow cytometry with imaging features of microscopy, is rapidly advancing as a cell imaging platform that overcomes ...
Ivan A. Vorobjev +4 more
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Multicolor quantitative confocal imaging cytometry
Nature Methods, 2017Multicolor 3D quantitative imaging of large tissue volumes is necessary to understand tissue development and organization as well as interactions between distinct cell types in situ. However, tissue imaging remains technically challenging, particularly imaging of bone and marrow.
Daniel L Coutu +3 more
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Applications of Aptamers in Flow and Imaging Cytometry
2016Aptamers compete with antibodies in many applications, in which high-affinity and specificity ligands are needed. In this regard, fluorescence-tagged aptamers have gained applications in flow and imaging cytometry for detecting cells expressing distinct antigens.
Arthur A. Nery +4 more
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1994
Prognosis in tumors depends on various potentialities of the neoplasm. Morphological characteristics of parenchyma, cells and nuclei have proven to reflect malignant behavior and are used to evaluate neoplasms with several grading systems. However, the descriptive and often subjective nature of such systems sometimes may result in disturbingly low ...
Doris Mack, Gerhard W. Hacker
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Prognosis in tumors depends on various potentialities of the neoplasm. Morphological characteristics of parenchyma, cells and nuclei have proven to reflect malignant behavior and are used to evaluate neoplasms with several grading systems. However, the descriptive and often subjective nature of such systems sometimes may result in disturbingly low ...
Doris Mack, Gerhard W. Hacker
openaire +2 more sources