Enzyme Engineering for In Situ Immobilization [PDF]
Molecules, 2016 Enzymes are used as biocatalysts in a vast range of industrial applications. Immobilization of enzymes to solid supports or their self-assembly into insoluble particles enhances their applicability by strongly improving properties such as stability in changing environments, re-usability and applicability in continuous biocatalytic processes.
Rehm, Fabian BH +2 more
openaire +5 more sources
Monitoring single membrane protein dynamics in a liposome manipulated in solution by the ABELtrap [PDF]
, 2011 FoF1-ATP synthase is the essential membrane enzyme maintaining the cellular level of adenosine triphosphate (ATP) and comprises two rotary motors. We measure subunit rotation in FoF1-ATP synthase by intramolecular Foerster resonance energy transfer (FRET) between two fluorophores at the rotor and at the stator of the enzyme.
arxiv +1 more source
Construction and investigation of multi-enzyme immobilized matrix for the production of HFCS.
PLoS ONEEnzymes are biological molecules that act as catalysts and speed up the biochemical reactions. The world's biotechnological ventures are development of enzyme productiveness, and advancement of novel techniques for thriving their shelf existence ...
Sabbir Janee +7 more
doaj +1 more source
Heliyon, 2023 Enzyme immobilization is a powerful tool for protecting enzymes from harsh reaction conditions and improving enzyme activity, stability, and reusability.
Mustafa Zeyadi, Yaaser Q. Almulaiky
doaj
Immobilization of bromelain on cobalt-iron magnetic nanoparticles (CoFe2O4) for casein hydrolysis
Revista Colombiana de Química, 2020 By means of recycling an enzyme, bromelain was used in casein hydrolysis facilitated by a nanobiocatalyst consisting of bromelain, CoFe2O4 magnetic nanoparticles, chitosan, and glutaraldehyde.
José Mauricio García Colmenares +1 more
doaj +1 more source
Investigations on vinylene carbonate. V. Immobilization of alkaline phosphatase onto LDPE films cografted with vinylene carbonate and N-vinyl-N-methylacetamide [PDF]
, 1993 Low-density polyethylene (LDPE) films cografted with vinylene carbonate (VCA) and N-vinyl-N-methylacetamide (VIMA) were studied as a matrix for the immobilization of the enzyme alkaline phosphatase (ALP) either by direct fixation or by inserting spacers.
Bantjes, Adriaan +2 more
core +4 more sources
An Insight in Developing Carrier-Free Immobilized Enzymes
Frontiers in Bioengineering and Biotechnology, 2022 Enzymes play vital roles in all organisms. The enzymatic process is progressively at its peak, mainly for producing biochemical products with a higher value.
V. Chauhan +6 more
semanticscholar +1 more source
Immobilization of Enzymes in Protein Films [PDF]
, 2020 Heterogeneous biocatalysis usually involves the use of immobilized enzymes on solid supports. Enzymes have suitable properties in terms of efficiency and selectivity for use as immobilized catalysts. Different approaches have been developed for effective immobilization, including adsorption, covalent binding, entrapment, encapsulation, and cross ...
Sanchez-deAlcazar, D +2 more
openaire +4 more sources
Monitoring the rotary motors of single FoF1-ATP synthase by synchronized multi channel TCSPC [PDF]
, 2007 Confocal time resolved single-molecule spectroscopy using pulsed laser excitation and synchronized multi channel time correlated single photon counting (TCSPC) provides detailed information about the conformational changes of a biological motor in real time.
arxiv +1 more source
Accelerated search kinetics mediated by redox reactions of DNA repair enzymes [PDF]
Biophysical Journal, volume 96, pp 3949-3958, (2009), 2009 A Charge Transport (CT) mechanism has been proposed in several papers (e.g., Yavin, et al. PNAS, v102 3546 (2005)) to explain the localization of Base Excision Repair (BER) enzymes to lesions on DNA. The CT mechanism relies on redox reactions of iron-sulfur cofactors that modify the enzyme's binding affinity.
arxiv +1 more source