Performance Improvement of Ca-Alginate Bead Cross- Linked Laccase from Trametes versicolor IBL-04
BioResources, 2015 Extracellular laccase was produced by Trametes versicolor IBL-04 using corn cobs as a substrate under pre-optimized culture conditions. A 64-kDa laccase enzyme was purified and immobilized on calcium alginate beads using glutaraldehyde as a cross-linking
Sadia Noreen +3 more
doaj +1 more source
Kinetics of b-galactosidase immobilized on polysiloxanepolyvinyl alcohol magnetic composite – POS-PVAM [PDF]
, 2007 b-Galactosidase is an enzyme with a wide industrial application, mostly in the hydrolysis of lactose and, more recently, in the synthesis of oligosaccharides. Several advantages are associated with the application of immobilized enzymes. In this work,
Balcão, V. M. +4 more
core
Synthesis of heparosan oligosaccharides by Pasteurella multocida PmHS2 single-action transferases [PDF]
, 2012 Pasteurella multocida heparosan synthase PmHS2 is a dual action glycosyltransferase that catalyzes the polymerization of heparosan polymers in a non-processive manner.
Boeriu, C.G. +3 more
core +2 more sources
FEBS Open Bio, EarlyView.Representation of the suggested mode of action of lactoferrin (Lf) in nonsmall cell lung cancer (NSCLC) A549 cells. Lf induces activation of caspase‐3 by activating p53 and AChE leading to decreased ACh concentrations. In turn, ACh signaling leads to activation of VEGF and AKT and blocking of caspase‐3.
Stuti Goel +9 more
wiley +1 more source
Biochemistry Research International, 2022 Enzyme immobilization is a powerful method to improve the stability, reuse, and enzymatic properties of enzymes. The immobilization of the α-amylase enzyme from Aspergillus fumigatus on a chitin-bentonite (CB) hybrid has been studied to improve its ...
Ezra Rheinsky Tiarsa +5 more
doaj +1 more source
Whey protein hydrolysis with free and immobilized Alcalase®: effects of operating parameters on the modulation of peptide profiles obtained [PDF]
, 2016 In this work, the effects of operating parameters on the modulation of peptide profiles obtained during enzymatic hydrolysis of whey protein concentrates (WPC) with a commercial enzyme (Alcalase 2.4L AF) were analyzed.
Ceruti, Roberto Julio +5 more
core +1 more source
SmallTalk: a novel small‐sized fusion tag for peptide expression and purification
FEBS Open Bio, EarlyView.The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq +3 more
wiley +1 more source
Catalytic Properties and Immobilization Studies of Catalase from Malva sylvestris L.
Journal of Chemistry, 2013 Catalase was partially purified from Malva sylvestris L. and immobilized onto chitosan. Then, its catalytic properties were investigated. (NH4)2SO4 precipitation and dialysis were performed in the extracted enzyme. Further purification was performed with
G. Arabaci, A. Usluoglu
doaj +1 more source
Immobilization of Trichoderma harzianum α-Amylase on Treated Wool: Optimization and Characterization
Molecules, 2014 α-Amylase from Trichoderma harzianum was covalently immobilized on activated wool by cyanuric chloride. Immobilized α-amylase exhibited 75% of its initial activity after 10 runs.
Saleh A. Mohamed +3 more
doaj +1 more source
Repeated batch for dye degradation in an airlift bioreactor by laccase entrapped in copper alginate [PDF]
, 2017 A repeated batch of synthetic dye decolorization was efficiently demonstrated in a 5 L airlift bioreactor. A laccase from Ganoderma sp. KU-Alk4, degrading commercial aromatic dyes was selected. The crude enzyme extract expressed laccase activity, and was
Chitradon, L. +7 more
core +1 more source