Results 51 to 60 of about 1,001,507 (301)

Hidrólise da lactose e síntese de galactooligossacarídeos utilizando B-galactosidase imobilizada em suportes à base de quitosana [PDF]

, 2014
Tese (doutorado) - Universidade Federal de Santa Catarina, Centro Tecnológico, Programa de Pós-Graduação em Engenharia de Alimentos, Florianópolis, 2014.A ß-galactosidase (E.C 3.2.1.23) é uma das enzimas mais empregadas na indústria de alimentos sendo ...
Klein, Manuela Poletto
core  

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Catalytic Properties and Immobilization Studies of Catalase from Malva sylvestris L.

Journal of Chemistry, 2013
Catalase was partially purified from Malva sylvestris L. and immobilized onto chitosan. Then, its catalytic properties were investigated. (NH4)2SO4 precipitation and dialysis were performed in the extracted enzyme. Further purification was performed with
G. Arabaci, A. Usluoglu
doaj   +1 more source

Preparation and characterization of κ-carrageenase immobilized onto magnetic iron oxide nanoparticles

Electronic Journal of Biotechnology, 2016
Background: Carboxyl-functionalized magnetic nanoparticles were synthesized via chemical co-precipitation method and modified with oleic acid which was oxidized by potassium permanganate, and κ-carrageenase from Pseudoalteromonas sp.
Anfeng Xiao, Caiyun Xu, Yan Lin, Hui Ni, Yanbing Zhu, Huinong Cai   +5 more
doaj   +1 more source

Importin 7 mediates the nuclear import of HIV‐1 integrase via a specific interacting interface

FEBS Open Bio, EarlyView.
HIV‐1 integrase enables viral DNA integration into the host genome. By binding to the core domain of the host protein Importin 7 via its C‐terminal domain, the integrase is transported across the nuclear membrane into the nucleus, where integration of the viral genome into host DNA takes place. This translocation is a critical step for subsequent viral
Juana Bana, Avigail Yariv, Tal Oppenheim, Hadar Amartely, Dana Reichmann, Oded Livnah   +5 more
wiley   +1 more source

Immobilized β-galactosidase BgaC from Bifidobacterium adolescentis retains stability and activity during repeated cycles of use

Applied Microbiology and Biotechnology
β-Galactosidase enzymes catalyze the hydrolysis of terminal non-reducing β-D-galactose residues in β-galactosides. These enzymes are important in producing lactose-free dairy products, reducing the lactose content of whey in dairy products, and for ...
Daniel Mehabie Mulualem, Orla Dwan, Michelle Kilcoyne, Conor O’Byrne, Aoife Boyd   +4 more
doaj   +1 more source

Performance Improvement of Ca-Alginate Bead Cross- Linked Laccase from Trametes versicolor IBL-04

BioResources, 2015
Extracellular laccase was produced by Trametes versicolor IBL-04 using corn cobs as a substrate under pre-optimized culture conditions. A 64-kDa laccase enzyme was purified and immobilized on calcium alginate beads using glutaraldehyde as a cross-linking
Sadia Noreen, Muhammad Asgher, Fatma Hussain, Ahsan Iqbal   +3 more
doaj   +1 more source

Optimization and efficacy studies of Laccase immobilized on Zein-Polyvinyl pyrrolidone nano fibrous membrane in decolorization of Acid Red 1

Water Science and Technology, 2021
Azo dyes are widely used in textile industries. A significant portion of these recalcitrant dyes are being discharged to the natural waters. Due to their low biodegradability they pose serious pollution problems if untreated.
G. Ranimol, Chinju Paul, Swetha Sunkar
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

One-step purification and immobilization of UDP-glycosyltransferase with modified Fe3O4 for the synthesis of ginsenoside Rh2

Xi'an Gongcheng Daxue xuebao
The disadvantages of high purification cost and difficult reuse of free enzymes restrict their application in the green biochemical industry, and immobilized enzymes with good performance are the necessary conditions for their industrialization.
LIU Xiaochen, YUE Junsong, WU Zhansheng, LI Zhiyan, TU Min, SHI Huaiqi, FAN Daidi   +6 more
doaj   +1 more source