Results 71 to 80 of about 2,310,619 (425)

Cytoplasmic delivery of antibodies through grafting a functional single complementarity‐determining region loop

open access: yesFEBS Letters, EarlyView.
We achieved cytoplasmic delivery of non‐cell‐penetrating IgGs by grafting a single functional complementarity‐determining region 1 (CDR1) from the light chain variable region (VL) of the cell‐internalizable 3D8 antibody. The engineered IgG acquired cell‐penetrating ability while maintaining antigen affinity, highlighting CDR1 grafting as a promising ...
Yerin Jeon   +5 more
wiley   +1 more source

SOD activity of immobilized enzyme mimicking complexes [PDF]

open access: yes, 2003
A binuclear, imidazolato-bridged, possible superoxide dismutase-mimicking complex (Cu(II)-diethylenetriamino-μ-imidazolato-Zn(II)-tris-aminoethylamine-triperchlorate) was prepared and immobilized on silica gel or among the layers of montmorillonite.
Hernádi, Klára   +4 more
core  

Dissipative particle dynamics simulation study on ATRP-brush modification of variably shaped surfaces and biopolymer adsorption [PDF]

open access: yesarXiv, 2022
We present a dissipative particle dynamics (DPD) simulation study on the surface modification of initiator embedded microparticles (MPs) of different shapes via atom transfer radical polymerization (ATRP) brush growth. The surface-initiated ATRP-brush growth leads to the formation of a more globular MP shape. We perform the comparative analysis of ATRP-
arxiv  

Unlocking the potential of tumor‐derived DNA in urine for cancer detection: methodological challenges and opportunities

open access: yesMolecular Oncology, EarlyView.
Urine is a rich source of biomarkers for cancer detection. Tumor‐derived material is released into the bloodstream and transported to the urine. Urine can easily be collected from individuals, allowing non‐invasive cancer detection. This review discusses the rationale behind urine‐based cancer detection and its potential for cancer diagnostics ...
Birgit M. M. Wever   +1 more
wiley   +1 more source

Performance Improvement of Ca-Alginate Bead Cross- Linked Laccase from Trametes versicolor IBL-04

open access: yesBioResources, 2015
Extracellular laccase was produced by Trametes versicolor IBL-04 using corn cobs as a substrate under pre-optimized culture conditions. A 64-kDa laccase enzyme was purified and immobilized on calcium alginate beads using glutaraldehyde as a cross-linking
Sadia Noreen   +3 more
doaj   +1 more source

Entrapment of glucoamylase by sol-gel technique in PhTES/TEOS hybrid matrixes [PDF]

open access: yes, 2007
Mesoporous silica particles were prepared by the sol-gel method from different alkoxysilane precursors and used as a host matrix for encapsulation of glucoamylase, an enzyme widely used in fermentative industry.
Dudás, Zoltán Imre   +2 more
core  

Acetylcholinesterase immobilization and characterization, and comparison of the activity of the porous silicon-immobilized enzyme with its free counterpart

open access: yesBioscience Reports, 2016
The physically adsorbed acetylcholinesterase on mesoporous silicon surface is presented. The catalytic behavior of immobilized enzyme was assessed by spectrophotometric bioassay. The immobilization enhanced the reusability, shelf life and thermal as well
M. Saleem, M. Rafiq, S. Seo, K. Lee
semanticscholar   +1 more source

An enzyme‐linked immunosorbent assay (ELISA)‐based activity assay for AMP‐activated protein kinase (AMPK)

open access: yesFEBS Open Bio, EarlyView.
Measuring AMP‐activated protein kinase (AMPK) activity in vitro is crucial for testing AMPK activators or inhibitors with therapeutic potential. Here, we report an enzyme‐linked immunosorbent assay (ELISA)‐based AMPK activity assay with simple steps and high sensitivity, which offers a simple, robust, and cost‐effective alternative to traditional ...
Trezze P. Nguyen, Shangze Lyu, Yang Liu
wiley   +1 more source

Optimization and efficacy studies of Laccase immobilized on Zein-Polyvinyl pyrrolidone nano fibrous membrane in decolorization of Acid Red 1

open access: yesWater Science and Technology, 2021
Azo dyes are widely used in textile industries. A significant portion of these recalcitrant dyes are being discharged to the natural waters. Due to their low biodegradability they pose serious pollution problems if untreated.
G. Ranimol, Chinju Paul, Swetha Sunkar
doaj   +1 more source

Kinetics of b-galactosidase immobilized on polysiloxanepolyvinyl alcohol magnetic composite – POS-PVAM [PDF]

open access: yes, 2007
b-Galactosidase is an enzyme with a wide industrial application, mostly in the hydrolysis of lactose and, more recently, in the synthesis of oligosaccharides. Several advantages are associated with the application of immobilized enzymes. In this work,
Balcão, V. M.   +4 more
core  

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