Results 161 to 170 of about 160,648 (208)
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Stereospecific hydrolysis by soluble and immobilized lipases
Biotechnology and Bioengineering, 1982AbstractStereospecific hydrolysis of insoluble monoesters by lipases are reported. Among the lipases tested, porcine pancreatic lipase was the most stereospecific when acting on 3‐chloro–2‐methyl propanol propionate. When the chain length of the acid was enhanced, the stereospecificity decreased.
J, Lavayre, J, Verrier, J, Baratti
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Production of flavor esters by immobilized lipase
Biotechnology Letters, 1987Candidacylindracea lipase adsorbed to silica gel produced a variety of flavor esters when hydrated and shaken in n-heptane containing substrates. Scale-up production of ethyl butyrate was examined in a packed column with recycling of n-hexane containing substrates. Increased substrate concentrations were stimulatory up to a point after which inhibition
Gillies, B., Yamazaki, H., Armstrong, D.
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Preparation and properties of immobilized lipoprotein lipase
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1980Purified bovine milk lipoprotein lipase has been covalently attached to CH-Sepharose with water-soluble carbodiimide. The immobilized enzyme retained enzymic activity and was stimulated 7-fold by the addition of human apolipoprotein C-II. Both [3H]heparin and 125I-labeled apolipoprotein C-II bound to the immobilized enzyme; unlabeled heparin and ...
N, Matsuoka, K, Shirai, R L, Jackson
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Ultrafiltration membranes as carriers for lipase immobilization
Enzyme and Microbial Technology, 1990The feasibility of directly incorporating lipase from Rhizopus in the interior of poly(vinyl chloride) ultrafiltration membranes during the phase inversion process for their manufacturing has been demonstrated. The obtained membranes used for plant oil hydrolysis have shown better time stability as compared with those of lipase immobilized by ...
M, Rucka, B, Turkiewicz
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Lipase immobilized in organic-inorganic matrics
Journal of Sol-Gel Science and Technology, 1997Enzyme lipase was immobilized with ferrite powder and deposited in layers on glass slides from lipase to a solution of silicone alkoxides. The highest hydrolytical activity was observed with the magnetic lipase prepared by mixing the paste of ferrite powder and lipase with tetramethoxysilane, 3-aminopropyltriethoxysilane and propyltrimethoxysilane.
Gabriela Kuncová, Miroslav Šivel
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Preparation and properties of immobilized papain and lipase
Biotechnology and Bioengineering, 1977AbstractPapain and lipase were immobilized on derivatized Sepharose 4‐B. The activated agarose had a binding capacity of 1.2 μmol amino groups/ml packed agarose or 17 mg proteins/g dry agarose. The immobilized enzyme preparations were tested for the effects of pH of assay, temperature of assay, and substrate concentrations. The effect of 6M urea on the
A. Kilara, K. M. Shahani, Fred W. Wagner
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Evaluation of cellulose-binding domain fused to a lipase for the lipase immobilization
Biotechnology Letters, 2004A cellulose-binding domain (CBD) fragment of a cellulase gene of Trichoderma hazianum was fused to a lipase gene of Bacillus stearothermophilus L1 to make a gene cluster for CBD-BSL lipase. The specific activity of CBD-BSL lipase for oil hydrolysis increased by 33% after being immobilized on Avicel (microcrystalline cellulose), whereas those of CBD-BSL
Sangpill, Hwang +7 more
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Rational Design of Immobilized Lipases and Phospholipases
2012Immobilization of lipases and phospholipases on, mainly, water insoluble carriers, helps in their economic reuse and in the development of continuous bioprocesses. Design of efficient lipases and phospholipases-immobilized system is rather a difficult task.
Alberto, Del Monte-Martínez +1 more
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Immobilization of Candida rugosa lipase and some properties of the immobilized enzyme
Enzyme and Microbial Technology, 1993Lipase (triacylglycerol ester hydrolase, E.C.3.1.1.3) from Candida rugosa has been immobilized on commercially available microporous polypropylene. The enzyme was rapidly adsorbed on the support, and more than 60% of the soluble activity disappeared from the medium after 1 min of incubation at room temperature. A recovery of immobilized activity of 21%
Montero, Sol +9 more
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Preparation and Properties of Lipases Immobilized on Different Supports
Artificial Cells, Blood Substitutes, and Biotechnology, 2004Porcine pancreatic lipase and Candida cylindracea lipase were immobilized on Celite and Amberlite IRA-938. Activities and stabilities of immobilized lipases were investigated. The immobilized lipase derivatives on Celite exhibited grater residual activity and more resistance to thermal inactivation than their immobilized counterpart on Amberlite IRA ...
Sagiroglu, A, Kilinc, A, Telefoncu, A
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