Results 21 to 30 of about 202,164 (266)

Production of AFB1 High-Specificity Monoclonal Antibody by Three-Stage Screening Combined with the De-Homologation of Antibodies and the Development of High-Throughput icELISA

Toxins, 2023
To achieve accurate detection of AFB1 toxin content in agricultural products and avoid false-positive rates in the assays, the specificity of mAbs is critical. We improved the specificity of the prepared monoclonal antibodies by modifying the traditional
Chengchen Pang, Qiang Liu, Lin Chen, Bei Yuan, Chuanyun Zha, Kunying Nie, Haitao Xu, Keyun Ren, Chunlei Yu, Yemin Guo, Qingqing Yang   +10 more
doaj   +1 more source

Label-Free Biosensors Based onto Monolithically Integrated onto Silicon Optical Transducers

Chemosensors, 2018
The article reviews the current status of label-free integrated optical biosensors focusing on the evolution over the years of their analytical performance.
Michailia Angelopoulou, Sotirios Kakabakos, Panagiota Petrou   +2 more
doaj   +1 more source

Human monoclonal antibodies isolated from a primary pneumococcal conjugate Vaccinee demonstrates the expansion of an antigen-driven Hypermutated memory B cell response

BMC Infectious Diseases, 2018
Background Community-acquired pneumonia is a leading infectious cause of hospitalization. A few vaccines exist to prevent pneumococcal disease in adults, including a pneumococcal polysaccharide unconjugated vaccine and a protein conjugated polysaccharide
Zhifeng Chen, Kara S. Cox, Aimin Tang, Jeanette Roman, Malorie Fink, Robin M. Kaufhold, Liming Guan, Andy Xie, Melissa A. Boddicker, Debra Mcguinness, Xiao Xiao, Hualin Li, Julie M. Skinner, Thorsten Verch, Mary Retzlaff, Kalpit A. Vora   +15 more
doaj   +1 more source

Anthracycline-Functionalized Dextran as a New Signal Multiplication Tagging Approach for Immunoassay

Biosensors, 2023
The most used kind of immunoassay is enzyme-linked immunosorbent assay (ELISA); however, enzymes suffer from steric effects, low stability, and high cost.
Fatema Kaladari, Naoya Kishikawa, Ai Shimada, Mahmoud El-Maghrabey, Naotaka Kuroda   +4 more
doaj   +1 more source

Immunoassay of Pesticides

Journal of AOAC INTERNATIONAL, 1991
Abstract Determination of the presence and levels of pesticide residues In food Is fundamental In monitoring and regulatory programs. Residues are separated from the food matrix by solvent extraction, followed by cleanup steps. The residues are most often Identified and quantltated by instrumental analysis, usually liquid or gas ...
B M, Kaufman, M, Clower
openaire   +2 more sources

Comparison of Six Serological Immunoassays for the Detection of SARS-CoV-2 Neutralizing Antibody Levels in the Vaccinated Population

Viruses, 2022
Neutralizing antibody (NAb) detection is critical for evaluating herd immunity and monitoring the efficacy of vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).
Hee-Jung Lee, Jin Jung, Ji Hyun Lee, Dong-Gun Lee, Young Bong Kim, Eun-Jee Oh   +5 more
doaj   +1 more source

Cell‐cycle‐specific lesion evolution rather than inhibition of double‐strand‐break repair underpins cisplatin radiosensitization

Molecular Oncology, EarlyView.
We analyze cisplatin–DNA adducts (CDAs) and double‐strand breaks (DSBs) in a cell‐cycle‐dependent manner. We find that CDAs form similarly across all cell cycle phases. DSBs arise only in S‐phase. CDAs might not directly impair DSB repair, but S‐phase DSB lesions evolve in the presence of CDAs and disrupt repair in G2, also causing radiosensitization ...
Ye Qiu, Xixi Lin, Emil Mladenov, Veronika Mladenova, Jürgen Thomale, Ali Sak, Yao Wang, Yunxuan Deng, Eleni Gkika, Martin Stuschke, George Iliakis   +10 more
wiley   +1 more source

Nanotechnology and Immunoassay

Clinical Chemistry, 2007
The roots of nanotechnology can be traced back to colloid science in the mid-19th century. Francesco Selmi, often credited with the first true research in the field, studied the behavior of various metallic and acidic emulsions in the 1840s (1). Shortly thereafter, Michael Faraday and Thomas Graham, two scientific pioneers known primarily for their ...
Samuel I, Stolper, Sonny S, Mark, Jason Y, Park, Larry J, Kricka   +3 more
openaire   +2 more sources

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Clinical Validation of Plasma p‐217tau in Neurological Diseases

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Plasma p‐217tau is a minimally invasive but specific biomarker for diagnosing Alzheimer's disease (AD). However, its disease specificity remains to be clinically evaluated. We validated the reliability of the p‐217tau biomarker in 12 other neurological diseases.
Takeshi Kawarabayashi, Takumi Nakamura, Ryoma Takahashi, Tetsuya Ueda, Seiji Kinoshita, Chikage Uchida, Takashi Sugawara, Kentaro Hashimoto, Kunihiko Ishizawa, Masakuni Amari, Hiroo Kasahara, Yoshio Ikeda, Masamitsu Takatama, Mikio Shoji   +13 more
wiley   +1 more source