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Immunoblotting and dot blotting
A variety of methods has been available for many years for the analytical separation of mixtures of proteins into their component parts by electrophoresis in a gel, usually agarose or polyacrylamide. Popular techniques are: zonal electrophoresis in agarose gel or on cellulose acetate membranes; discontinuous electrophoresis in polyacrylamide gel (PAGE);
D I Stott, D I Stott
exaly +3 more sources
Measurement of the IgG Avidity Index in the Diagnosis of Clinical Toxocariasis Patients
Toxocara spp. are parasitic nematodes responsible for human toxocariasis, a common zoonotic helminth infection. The five main features of human toxocariasis are the classical ocular toxocariasis and visceral larva migrans syndrome, followed by covert ...
Estelle Menu +4 more
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Gestational exposure of mice to valproic acid (VPA) is one currently used experimental model for the investigation of typical failure symptoms associated with autism spectrum disorder (ASD).
Cintia Klaudia Finszter +7 more
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The abundance of a transfer RNA-derived RNA fragment small RNA subpopulation is enriched in cauda spermatozoa [PDF]
The small RNA (sRNA) landscape of mammalian spermatozoa is considerably altered as these gametic cells migrate through the segment specific microenvironments of the epididymis.
Simone J. Stanger +6 more
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A Preliminary Study on the Antigenic Proteins of Linguatula serrata Nymphal Stage in Sheep with Visceral Linguatulosis [PDF]
Scant information is available on the immunological aspect of Linguatula serrata causing linguatulosis in humans and animals. The present study aimed to analyze the content of crude somatic extracts and excretory-secretory products of L.
Alireza Alborzi, S Bahrami, F Khajeh
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The pattern of allergic sensitization by the skin prick test and immunoblotting method among patients with atopic dermatitis in 2018 [PDF]
Background: Atopic dermatitis (AD) is a common allergic disorder. Detection of responsible pathogenic allergens in AD patients by reliable methods has a fundamental role in the prevention, management, and treatment of AD.
Mohammad Sadegh Naghizadeh +4 more
doaj
Von Willebrand factor (VWF) is a 500‐ to 15 000‐kDa multimeric protein circulating in the blood. When VWF has a higher molecular weight, its hemostatic activity is greater.
Yuka Eura, Koichi Kokame
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Immunoblotting (western blotting) is a rapid and sensitive assay for the detection and characterization of proteins that works by exploiting the specificity inherent in antigen-antibody recognition. It involves the solubilization and electrophoretic separation of proteins, glycoproteins, or lipopolysaccharides by gel electrophoresis, followed by ...
Sean, Gallagher, Deb, Chakavarti
openaire +2 more sources
Comprehensive Optimization of Western Blotting
Western blotting is one of the most extensively used techniques in the biomedical field. However, it is criticized by many researchers due to its considerable time consumption, multiple steps, and low method results.
Dishiwen Liu +3 more
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Objective of the research was to use immunoblotting for studies of epitope targeting in monoclonal antibodies to 200 kDa Burkholderia pseudomallei antigen, which are synthesized by hybridomas-producers from the two collections in the laboratory of ...
I. I. Korsakova +5 more
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