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Immunoblotting and dot blotting

open access: yesJournal of Immunological Methods, 1989
A variety of methods has been available for many years for the analytical separation of mixtures of proteins into their component parts by electrophoresis in a gel, usually agarose or polyacrylamide. Popular techniques are: zonal electrophoresis in agarose gel or on cellulose acetate membranes; discontinuous electrophoresis in polyacrylamide gel (PAGE);
D I Stott, D I Stott
exaly   +3 more sources

Measurement of the IgG Avidity Index in the Diagnosis of Clinical Toxocariasis Patients

open access: yesPathogens, 2021
Toxocara spp. are parasitic nematodes responsible for human toxocariasis, a common zoonotic helminth infection. The five main features of human toxocariasis are the classical ocular toxocariasis and visceral larva migrans syndrome, followed by covert ...
Estelle Menu   +4 more
doaj   +1 more source

Early cellular and synaptic changes in dopaminoceptive forebrain regions of juvenile mice following gestational exposure to valproate

open access: yesFrontiers in Neuroanatomy, 2023
Gestational exposure of mice to valproic acid (VPA) is one currently used experimental model for the investigation of typical failure symptoms associated with autism spectrum disorder (ASD).
Cintia Klaudia Finszter   +7 more
doaj   +1 more source

The abundance of a transfer RNA-derived RNA fragment small RNA subpopulation is enriched in cauda spermatozoa [PDF]

open access: yesExRNA, 2020
The small RNA (sRNA) landscape of mammalian spermatozoa is considerably altered as these gametic cells migrate through the segment specific microenvironments of the epididymis.
Simone J. Stanger   +6 more
doaj   +1 more source

A Preliminary Study on the Antigenic Proteins of Linguatula serrata Nymphal Stage in Sheep with Visceral Linguatulosis [PDF]

open access: yesArchives of Razi Institute, 2022
Scant information is available on the immunological aspect of Linguatula serrata causing linguatulosis in humans and animals. The present study aimed to analyze the content of crude somatic extracts and excretory-secretory products of L.
Alireza Alborzi, S Bahrami, F Khajeh
doaj   +1 more source

The pattern of allergic sensitization by the skin prick test and immunoblotting method among patients with atopic dermatitis in 2018 [PDF]

open access: yesMedical Laboratory Journal, 2023
Background: Atopic dermatitis (AD) is a common allergic disorder. Detection of responsible pathogenic allergens in AD patients by reliable methods has a fundamental role in the prevention, management, and treatment of AD.
Mohammad Sadegh Naghizadeh   +4 more
doaj  

Commonly used anti–von Willebrand factor antibody for multimer analysis cross‐reacts with fibronectin, which is difficult to distinguish from von Willebrand factor

open access: yesResearch and Practice in Thrombosis and Haemostasis, 2021
Von Willebrand factor (VWF) is a 500‐ to 15 000‐kDa multimeric protein circulating in the blood. When VWF has a higher molecular weight, its hemostatic activity is greater.
Yuka Eura, Koichi Kokame
doaj   +1 more source

Immunoblot Analysis [PDF]

open access: yesJournal of Visualized Experiments, 2008
Immunoblotting (western blotting) is a rapid and sensitive assay for the detection and characterization of proteins that works by exploiting the specificity inherent in antigen-antibody recognition. It involves the solubilization and electrophoretic separation of proteins, glycoproteins, or lipopolysaccharides by gel electrophoresis, followed by ...
Sean, Gallagher, Deb, Chakavarti
openaire   +2 more sources

Comprehensive Optimization of Western Blotting

open access: yesGels, 2023
Western blotting is one of the most extensively used techniques in the biomedical field. However, it is criticized by many researchers due to its considerable time consumption, multiple steps, and low method results.
Dishiwen Liu   +3 more
doaj   +1 more source

Utilization of Immunoblotting in Studies of Epitope Targeting in Monoclonal Antibodies to Melioidosis Agent Antigen 200 kDa

open access: yesПроблемы особо опасных инфекций, 2014
Objective of the research was to use immunoblotting for studies of epitope targeting in monoclonal antibodies to 200 kDa Burkholderia pseudomallei antigen, which are synthesized by hybridomas-producers from the two collections in the laboratory of ...
I. I. Korsakova   +5 more
doaj   +1 more source

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