Results 111 to 120 of about 323,380 (305)
Buffer Recipes for quantitative immunoblotting - rinsing and blocking agents v1
, 2017 These are the recipes for blocking and rinsing agents for the quantitative immunoblotting protocol from the Campbell Lab.
Doug Campbell, Natalie Donaher
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Advanced Science, EarlyView.We report a new thiolate‐reactive α,α‐gem‐dibromo lactam warhead that activates transcription factor Nrf2 and demonstrates anti‐inflammatory activities, which have implications in cancer, neurodegeneration, and cardiovascular diseases. RNA‐seq illuminated detailed transcriptional profiles, and chemical reactions with cysteine‐containing compounds ...
Beau R. Brummel +16 more
wiley +1 more source
Immunoblotting of cervical cancer tissue extracts.
, 2016 Whole (W), cytoplasmic (C), and nuclear (N) fractions were prepared from tissues of one adenocarcinoma (ADC) and five squamous cell carcinomas (SqCC), and GS28 expression in the fractions was examined by immunoblotting using the anti-GS28 antibody ...
Oh-Joo Kwon (3112221) +5 more
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Advanced Science, EarlyView.TRMT6‐mediated m1A modification in CDK9 mRNA enhances its mRNA stability and translation efficiency, thereby increasing the protein levels of CDK9. Upregulated CDK9 promotes the progression of HCC by elevating the levels of oncogenic factors including p‐STAT3, MCL1, and BCL‐2. On the other hand, CDK9 phosphorylates TARDBP at Ser254 to activate HBV core
Rui Zhang +12 more
wiley +1 more source
Immunoreactivity of canine, feline, and equine D‐dimer with antibodies to human D‐dimer
Journal of Veterinary Internal MedicineBackground Commercially available D‐dimer assays use antibodies against human D‐dimer, with limited sensitivity and specificity data in companion animals.
Juliet E. Brown +2 more
doaj +1 more source
Validation of microarray data by Immunoblotting.
, 2013 Immunoblotting analysis of selected proteins was performed on samples from control, DM and Serca2a-transduced hearts. A representative blot from 2–3 experiments is shown. sFRP4, secreted frizzled-related protein 4; DSC2, desmocollin 2.
Roger J. Hajjar (142214) +8 more
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Unraveling the Molecular Mechanisms Underlying Spontaneous Multipolar Mitosis Through CIN‐seq
Advanced Science, EarlyView.Multipolar mitosis, a hallmark of chromosomal instability (CIN), drives tumor heterogeneity but is challenging to study in live cells. Using CIN‐seq, a single‐cell multiomics method, we profiled rare CIN events and identified mechanisms associated with viable multipolar mitosis, including PTEN attenuation, Rho GTPase‐driven cytokinesis failure, and ...
Pin‐Rui Su +10 more
wiley +1 more source
Experimental PhysiologyWe examined how resistance exercise (RE), cycling exercise and disuse atrophy affect myosin heavy chain (MyHC) protein fragmentation. The 1boutRE study involved younger men (n = 8; 5 ± 2 years of RE experience) performing a lower body RE bout with vastus
Daniel L. Plotkin +16 more
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Effect of protein kinase N1 on the biological behavior of human glioma
Chinese Journal of Contemporary Neurology and Neurosurgery, 2020 Objective To study the expression of protein kinase N1 (PKN1) in glioma and its influence on the biological behavior of glioma cells. Methods The expression of PKN1 gene was verified via GEPIA database, and LN229, U87 and A172 gliomas cells were detected
Yu⁃bing HAO +5 more
doaj
Immunoblotting analysis of surface-exposed proteins.
, 2014 (A) Five recombinant SEPs purified from E. coli cell lysate were separated by 12% SDS-PAGE and stained by G-250 Coomassie Brilliant Blue. (B) Immunoblotting analysis of SEPs: lane M, protein molecular mass markers; lanes 1 to 5, rTolC, rPorin_4, rAdr1 ...
Xiaolu Xiong (436303) +5 more
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