SDS-PAGE and immunoblotting to assess whole-cell lysates from cell culture systems v1 [PDF]
Frances V Hundley +3 more
openalex +1 more source
NPM1 selectively sorts specific mRNAs into extracellular vesicles (EVs) by recognizing RNA motifs and forming phase‐separated condensates. These mRNA cargos—including EGFR—are then loaded via multivesicular bodies. This active sorting pathway, validated in EVs derived from both lung cancer cells and patient serum, reveals a specific mechanism for ...
Kaixiang Zhang +8 more
wiley +1 more source
Deubiquitinase UCH-L1 confers paclitaxel resistance via stabilizing PKM2 to promote glycolysis in triple-negative breast cancer. [PDF]
Chen X +9 more
europepmc +1 more source
Separation and characterization of sperm antigens using two-dimensional gel electrophoresis and immunoblotting [PDF]
Bei Xu
openalex
Identification of antigenic components of Toxoplasma gondii by an immunoblotting technique [PDF]
Paul Partanen +5 more
openalex +1 more source
This study integrates multi‐omics to reveal the critical role of UBE2T in driving malignancy and stromal co‐evolution in PDAC. UBE2T potentiates glycolysis by regulating p53 degradation via a positive feedback loop, thereby promoting histone H3 lysine 18 lactylation in CAFs and stromal deposition. The UBE2T inhibitor PGG represents a potential strategy
Yong Ma +16 more
wiley +1 more source
Protective role of fatty acid oxidation against epithelial barrier dysfunction in allergic asthma. [PDF]
Wang M +11 more
europepmc +1 more source
Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product
G. Evan, G. Lewis, G. Ramsay, J. Bishop
semanticscholar +1 more source
Immunoblotting validation of research antibodies generated against HS1-associated protein X-1 in the human neutrophil model cell line PLB-985. [PDF]
Peter J. Cavnar, Kristina Inman
openalex +1 more source
High light exposure triggers an epidermis‐specific remodeling of mitochondria and ER in Arabidopsis, driven by chloroplast‐derived signals. Live‐cell imaging shows that HL rapidly suppresses mitochondrial motility, followed by fusion‐driven elongation and ER cisternal expansion.
Evan R. Angelos +12 more
wiley +1 more source

