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Immunoblotting.

Cold Spring Harbor Protocols, 2020
Immunoblotting allows detection of a protein antigen immobilized on the protein-retaining membrane support such as nitrocellulose or polyvinylidene fluoride (PVDF).
Larisa Litovchick
semanticscholar   +3 more sources

Immunoblotting and Immunodetection

Current Protocols in Immunology, 2017
AbstractImmunoblotting (western blotting) is used to identify specific antigens recognized by polyclonal or monoclonal antibodies. This unit provides protocols for all steps, starting with solubilization of the protein samples, usually by means of SDS and reducing agents.
Duojiao Ni, Peng Xu, S. Gallagher
semanticscholar   +7 more sources

Immunoblotting and Immunodetection

Current Protocols in Immunology, 2011
AbstractImmunoblotting (western blotting) is used to identify specific antigens recognized by polyclonal or monoclonal antibodies. This unit provides protocols for all steps, starting with solubilization of the protein samples, usually by means of SDS and reducing agents.
S. Gallagher   +3 more
semanticscholar   +9 more sources

Grid-Immunoblotting

2009
Grid-Immunoblotting is a fast, simple, and efficient method for simultaneously testing multiple allergens utilizing small amount of antibody.
Olga, Yeliosof, R Hal, Scofield
openaire   +3 more sources

Immunoblotting Techniques

2005
Immunoblotting techniques use antibodies (or other specific ligands in related techniques) to identify target proteins among a number of unrelated protein species. They involve identification of protein target via antigen-antibody (or protein-ligand) specific reactions. Proteins are typically separated by electrophoresis and transferred onto membranes (
Barbara, Magi, Sabrina, Liberatori
openaire   +2 more sources

Immunoblot Detection

Current Protocols in Protein Science, 1996
AbstractImmunoblotting (often referred to as western blotting) is used to identify specific antigens recognized by polyclonal or monoclonal antibodies. Following electrophoretic separation of proteins and transfer from the gel to an appropriate membrane, the immobilized proteins are probed with specific antibodies to identify and quantitate any ...
openaire   +2 more sources

Immunoblotting for Calpastatin Expression

2019
Immunoblotting is a procedure routinely used to analyze calpastatin expression. However, immunoblotting alone may not be adequate for this task, since calpastatin isoforms can vary by tissue, can be modified by partial digestion, and can undergo posttranslational modifications.
Averna, Monica, De Tullio, Roberta
openaire   +3 more sources

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