Results 81 to 90 of about 171,680 (341)

Protocol for adhesion and immunostaining of lymphocytes and other non-adherent cells in culture

BioTechniques, 2017
Immunostaining of non-adherent cells is commonly performed after adhesion of cells onto microscope slides either using cytocentrifugation or with the help of charged coating substrates.
Matthew Tsang, Jennifer Gantchev, Feras M. Ghazawi, Ivan V. Litvinov   +3 more
doaj   +1 more source

Restoring NK Cell Cytotoxicity Post‐Cryopreservation via Synthetic Cells

Advanced Science, EarlyView.
In this study, it is shown that 1‐day co‐culture with activated T cells or IL‐2‐presenting synthetic cells significantly enhances NK cell motility and cytotoxicity in 2D and 3D. Notably, synthetic cells restore the impaired cytotoxic function of cryopreserved NK and CAR‐NK cells in 3D.
Xiangda Zhou, Sijia Zhang, Wenjuan Yang, Susanne Gonder, Zeinab Sadjadi, Nils Piernitzki, Alina Moter, Shulagna Sharma, Anne Largeot, Nadja Küchler, Lea Kaschek, Gertrud Schäfer, Eva C. Schwarz, Hermann Eichler, Evelyn Ullrich, Heiko Rieger, Oskar Staufer, Jérôme Paggetti, Etienne Moussay, Markus Hoth, Bin Qu   +20 more
wiley   +1 more source

Immunocytochemistry of pituitary tumors. [PDF]

Journal of Histochemistry & Cytochemistry, 1987
Pituitary tumors from 376 patients were investigated, using immunocytochemical techniques at the light and electron microscopic level, and autoradiography combined with immunocytochemistry for localizing somatostatin (SRIH) receptors. Prolactinomas, growth hormone-secreting adenomas causing acromegaly, and hormonally inactive adenomas were most ...
J C Reubi, H. R. Zenklusen, M Oberholzer, Jürgen Roth, M. Kasper, A M Landolt, Ph. U. Heitz   +6 more
openaire   +3 more sources

Protein kinase a distribution differentiates human glioblastoma from brain tissue [PDF]

, 2017
Brain tumor glioblastoma has no clear molecular signature and there is no effective therapy. In rodents, the intracellular distribution of the cyclic AMP (cAMP)-dependent protein kinase (Protein kinase A, PKA) R2Alpha subunit was previously shown to ...
Caretta, Antonio, D\u2019Avella, Domenico, Denaro, Luca, Mucignat-Caretta, Carla   +3 more
core   +2 more sources

PSMD14‐Mediated LDHA Deubiquitination Upregulates ACLY Expression via H3K18 Lactylation to Promote Lipid Synthesis and Pancreatic Cancer Progression

Advanced Science, EarlyView.
This study investigates the expression and functional role of the deubiquitinase PSMD14 in PC, with a focus on the mechanism by which it promotes lactate accumulation–induced histone lactylation, thereby driving aberrant lipid metabolism. These findings establish that dual targeting of PSMD14 and the glycolysis–lactylation signaling axis may represent ...
Ri‐Shang Lu, Li‐Kun Ren, Xiao‐Bin Fei, Song‐Bai Liu, Yong‐Jia Gao, Jun‐Yi Hou, Chi Wang, Peng Liu, Chang‐Hao Zhu, Xing Wang, Yao‐Zhen Pan   +10 more
wiley   +1 more source

Effects of a 10% carbamide peroxide bleaching agent on rat oral epithelium proliferation

Brazilian Dental Journal, 2002
The purpose of the present study was to evaluate the influence of short course topical application of carbamide peroxide on proliferating cell nuclear antigen (PCNA) immunohistochemical expression in the oral tongue mucosa of rats.
Albuquerque Rodrigo de Castro, Gomez Ricardo Santiago, Dutra Rodrigo Aliprandi, Vasconcellos Wallison Arthuso, Gomez Renato Santiago, Gomez Marcus Vinícius   +5 more
doaj  

Netrin‐1 and B‐cell maturation antigen expression in a large cohort of 361 lymphomas: sensitive and specific staining in plasmablastic lymphomas, and therapeutic perspectives

The Journal of Pathology: Clinical Research
Netrin‐1 and B‐cell maturation antigen (BCMA) are currently being evaluated as therapeutic targets in oncology. However, studies investigating their expression in mature human lymphoid malignancies are sparse.
Marie Donzel, Alexis Trecourt, Hervé Ghesquières, Thi‐Thuy‐Trinh Nguyen, Sara Dandash, Morgane Denis, Emeline Cros‐Perrial, Juliette Fontaine, Charles Dumontet, Alexandra Traverse‐Glehen   +9 more
doaj   +1 more source

Porphyromonas gingivalis lipopolysaccharide alters atherosclerotic-related gene expression in oxidized low-density-lipoprotein-induced macrophages and foam cells [PDF]

, 2011
The molecular mechanism between atherosclerosis formation and periodontal pathogens is not clear although positive correlation between periodontal infections and cardiovascular diseases has been reported.
de Winther, Demmer, Diya, Fischer, Gamble, Ghosh, Giacona, Gibson, Gitlin, Groeneweg, Janabi, Kleemann, Lalla, Li, Lu, Ma, Majdalawieh, Martin, Mikita, Oram, Qi, Robbesyn, Ross, Zhou   +23 more
core   +2 more sources

Cerebral Organoids with Integrated Endothelial Networks Emulate the Neurovascular Unit and Mitigate Core Necrosis

Advanced Science, EarlyView.
Fumadó Navarro et al. present an optimized strategy to generate vascular‐like networks that infiltrate within cerebral organoids with promising signs of blood‐brain barrier characteristics, preserved stiffness, uniform morphology, enhanced media diffusion, and reduced necrotic core.
Josep Fumadó Navarro, Siobhan Crilly, Wai Kit Chan, Shane Browne, John O. Mason, Catalina Vallejo‐Giraldo, Abhay Pandit, Mihai Lomora   +7 more
wiley   +1 more source

Ascites as a subject of studies in ovarian cancer

Сибирский онкологический журнал, 2019
Ovarian cancer is a highly heterogeneous disease characterized by multiple histological subtypes. High- grade serous ovarian carcinoma is the most common histological subtype of ovarian cancer.
A. B. Villert, L. A. Kolomiets, N. V. Yunusova, A. A. Ivanova   +3 more
doaj   +1 more source