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2003
Ressler first described in 1960 (1) a form of immunoelectrophoresis now called crossed immunoelectrophoresis (CIE) or two-dimensional immunoelectrophoresis, which was later improved by Laurell (2), Clarke and Freeman (3), and Weeke (4), among others.
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Ressler first described in 1960 (1) a form of immunoelectrophoresis now called crossed immunoelectrophoresis (CIE) or two-dimensional immunoelectrophoresis, which was later improved by Laurell (2), Clarke and Freeman (3), and Weeke (4), among others.
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Shape of the Precipitate in Immunoelectrophoresis
Nature, 1960IN immunoelectrophoresis, according to Grabar and Williams1, diffusion of the electrophoretically separated antigens towards the immune serum added in longitudinal basins parallel to the migration axis after the electrophoretic separation will result in the formation of precipitates in the gel, occupying varying positions between antigen and antibody ...
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Improved agarose for immunoelectrophoresis
Analytical Biochemistry, 1976Abstract Alkali-treatment of agarose was found to be a very simple method to produce charge-free agarose from commercially available agarose in high yield (about 90%) with highly reduced content of sulphate groups and very low electroendosmotic flow. Applications of the agarose in immunoelectrophoretic experiments are also described.
Bo Lönnerdal, Torgny Låås
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Mykosen, 1980
Summary: Antigenic extracts were prepared from the mycelium of two strains of Aspergillus fumigatus. Surface antigens were obtained from crude wall by detergent extraction and water‐soluble antigens were obtained from total, macerated mycelium.
V. Hearn, D. Mackenzie
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Summary: Antigenic extracts were prepared from the mycelium of two strains of Aspergillus fumigatus. Surface antigens were obtained from crude wall by detergent extraction and water‐soluble antigens were obtained from total, macerated mycelium.
V. Hearn, D. Mackenzie
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Immunoelectrophoresis of Cataractous Lenses
Archives of Ophthalmology, 1959The history of immunology of lens proteins goes back to Uhlenhuth, 1 who 55 years ago showed by precipitation tests the organ specificity of some of the lens components. This was verified later by Burky, Woods, and Woodhall. 2 They found that mainly the so-called α-crystallin was organspecific.
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Comparative analysis of gingival fluid and plasma by crossed immunoelectrophoresis.
Journal of Periodontal Research, 1980Samples of gingival fluid and plasma were taken from ten patients with varying severity of periodontal disease. Group I comprised seven patients with abundant plaque. Their mean pocket depth at the sampling sites was 5.6 ± 1.27 mm.
T. Tollefsen, E. Saltvedt
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Immunoelectrophoresis of the proteins of the corneal epithelium
Experimental Eye Research, 1963The proteins of the corneal epithelium are separated into a greater number of fractions by high-tension agar electrophoresis than by paper electrophoresis. The distribution of the different fractions is greatly influenced by the ionic strength of the buffer solution.
M. Rabaey, Jules François
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Electrophoresis and Immunoelectrophoresis.
Archives of Internal Medicine, 1971This book explains the basic principles of electrophoresis and immunoelectrophoresis, discusses specific applications of these techniques in the clinical laboratory, and is appended with technical details of each of the methods considered. The advantages of this text are numerous.
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Electrophoresis and Immunoelectrophoresis [PDF]
, 1979 For a detailed consideration of electrophoretic techniques and the factors which influence them the standard texts are recommended1-3. This discussion will be restricted to those aspects of electrophoresis which are most likely to affect the practical applications particularly as applied to proteins.
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, 1975
The concentration of an antigen partially identical to the rat brain synaptic membrane antigen D2, was measured in human cerebrospinal fluid. The method used was rocket‐line immunoelectrophoresis in agarose gel containing the detergent Triton X‐100, and ...
Ole Steen Jørgensen, E. Bock
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The concentration of an antigen partially identical to the rat brain synaptic membrane antigen D2, was measured in human cerebrospinal fluid. The method used was rocket‐line immunoelectrophoresis in agarose gel containing the detergent Triton X‐100, and ...
Ole Steen Jørgensen, E. Bock
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