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Crossed Immunoelectrophoresis and Electroimmunoassay of IgG
The Journal of Immunology, 1974Abstract Simple procedures for crossed immunoelectrophoresis and electroimmunoassay of IgG are described. It is shown how crossed immunoelectrophoresis can readily be used to demonstrate different types of IgG immune responses as well as the formation of complexes between IgG and other molecules.
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Theoretical analysis of immunoelectrophoresis
Journal of Theoretical Biology, 1962An analysis is made of the processes that lead to the formation of the initial zone of precipitation between antigen and antibody during immunoelectrophoresis. With the aid of certain simplifying assumptions, equations are developed which describe: (1) the concentration distribution of electrophoretically heterogeneous antigen after electrophoresis, (2)
Robert W. Taylor +2 more
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Journal of Immunological Methods, 1974
Abstract A modification of immunoelectrophoresis is described which facilitates the identification of precipitation arcs of individual proteins. The technique consists of simultaneous electrophoretic separation of the protein mixture and reference protein(s) on the same electrophoretic axis, followed by reaction with the antiserum.
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Abstract A modification of immunoelectrophoresis is described which facilitates the identification of precipitation arcs of individual proteins. The technique consists of simultaneous electrophoretic separation of the protein mixture and reference protein(s) on the same electrophoretic axis, followed by reaction with the antiserum.
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Immunoelectrophoresis for the Characterization of Allergen Extracts
2008Immunoelectrophoresis can be used for analysis of individual proteins in complex mixtures. The conditions involved in immunoelectrophoresis are mild, avoiding the risk of denaturation, and it is possible to perform relative quantification of individual components. The principle disadvantage is the dependence on rabbit antisera as reagents.
Jørgen Nedergaard Larsen +1 more
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Serology, Immunochemistry, and Immunoelectrophoresis
1994Immune phenomena play a vital role in the health and disease of higher animals. Among these phenomena are (1) the protection conferred on higher animals against certain viral- and bacterial-induced diseases, either by their survival of an initial attack of the disease or by the deliberate injection of a modified form of the disease-producing agent ...
Clifton E. Meloan, Yeshajahu Pomeranz
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Brain specific synaptosomal membrane proteins demonstrated by crossed immunoelectrophoresis
Journal of Neurochemistry, 1974O. Jørgensen, E. Bock
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Immunoelectrophoresis: A Method with Many Faces
2012Immunoelectrophoresis (IEP) was the first practical method that combined electrophoresis and immunoprecipitation for identifying and characterizing proteins within complex mixtures. Over the years, IEP has been extended to include a variety of techniques and, as a general name, has been applied to virtually any technique that involves electrophoresis ...
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