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Immunofluorescence Microscopy

Current Protocols, 2023
AbstractVisualizing fluorescence‐tagged molecules is a powerful strategy that can reveal the complex dynamics of the cell. One robust and broadly applicable method is immunofluorescence microscopy, in which a fluorescence‐labeled antibody binds the molecule of interest and then the location of the antibody is determined by fluorescence microscopy.
Domenico F, Galati, David J, Asai
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Immunofluorescence

Zeitschrift fur Rheumatologie, 2011
The existence of autoantibodies is characteristic of several rheumatic diseases and indicates impaired self-tolerance. The analysis and detection of these autoantibodies represent an important factor in the diagnosis of rheumatic diseases. Immunofluorescence is an important technique in basic science and also one of the most commonly used techniques ...
S, Lefèvre, U, Lange
  +6 more sources

Immunofluorescence Microscopy

2012
Immunofluorescence microscopy is a powerful technique that is widely used by researchers to assess both the localization and endogenous expression levels of their favorite proteins. The application of this approach to C. elegans, however, requires special methods to overcome the diffusion barrier of a dense, collagen-based outer cuticle.
Diane C, Shakes   +2 more
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Subnuclear immunofluorescence

2006
Despite their relatively small size and the fact that they are naturally non-adherent, it is possible to obtain good immunofluorescence staining of subnuclear structures in DT40. This, combined with their genetic tractability, provides a powerful combination for the study of DNA replication and repair.
David, Szüts, Julian E, Sale
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Cutaneous Immunofluorescence

Medical Clinics of North America, 1989
Immunofluorescence, especially direct immunofluorescence of skin, has been widely used for diagnosis of connective tissue diseases. It is principally of value for LE and may be used also as a prognostic test for SLE. It is of relatively little value for diagnosis of other connective tissue diseases and appears not to predict the outcome of ...
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Cutaneous Immunofluorescence

Clinics in Laboratory Medicine, 1986
This article provides an update on skin immunofluorescence as a useful laboratory procedure in the evaluation of diseases that have cutaneous manifestations. It also provides insight into some of the problems with submitting and processing specimens as well as the difficulties and variables encountered when interpreting positive results.
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Immunofluorescence Assay

The investigation of yellow fever suspected cases is important for both epidemiological surveillance and for the timely adoption of supportive therapy. The immunofluorescence assay (IFA), a technique based on the detection of specific targets by fluorescent dye-tagged antibodies, may be useful for the serological diagnosis of yellow fever and/or for ...
Andréa Nazaré Monteiro Rangel, da Silva   +1 more
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Multiplex Immunohistochemistry and Immunofluorescence: A Practical Update for Pathologists.

Modern Pathology, 2023
P. Harms   +8 more
semanticscholar   +1 more source

Optimizing Direct Immunofluorescence

2014
Immunofluorescence is a laboratory technique that utilizes a fluorophore-labeled antibody to detect immune complexes in tissue. Most of the labeled antibodies used in a clinical laboratory bind the conserved domains within each class of human antibodies, allowing them to detect a wide range of autoimmune complexes.
Ian D, Odell, Deborah, Cook
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Indirect immunofluorescence microscopy

2006
This is a standard protocol for the detection of intracellular proteins by indirect immunofluorescence microscopy in DT40. It has been used extensively to investigate the intracellular distribution of various proteins of the endocytic machinery.
Frank R, Wettey, Antony P, Jackson
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