Results 1 to 10 of about 13,448 (142)

Effect of IgG fragments in IVIG(pH4)on phagocytosis of sensitized erythrocytes by macrophage

Zhongguo shuxue zazhi, 2022
Objective To research the effect of the Fc, Fab and F(ab′)2 fragments of immunoglobulin G, the main components of Human Immunoglobulin(pH4) for Intravenous Injection(IVIG), on the phagocytic function of macrophages derived from THP-1 cells. Methods First
Liyuan ZHU, Wei ZHANG, Mingxia HOU, Qin LIU, Yuyan QIN, Li Ma, Changqing LI   +6 more
doaj   +1 more source

Strategies for the production of isotopically labelled Fab fragments of therapeutic antibodies in Komagataella phaffii (Pichia pastoris) and Escherichia coli for NMR studies.

PLoS ONE, 2023
The importance and fast growth of therapeutic monoclonal antibodies, both innovator and biosimilar products, have triggered the need for the development of characterization methods at high resolution such as nuclear magnetic resonance (NMR) spectroscopy.
Donald Gagné, Muzaddid Sarker, Geneviève Gingras, Derek J Hodgson, Grant Frahm, Marybeth Creskey, Barry Lorbetskie, Stewart Bigelow, Jun Wang, Xu Zhang, Michael J W Johnston, Huixin Lu, Yves Aubin   +12 more
doaj   +1 more source

The Fab region of IgG impairs the internalization pathway of FcRn upon Fc engagement

Nature Communications, 2022
Disrupting the association between the Immunoglobulin G constant fragment (Fc) and the neonatal Fc receptor (FcRn) by engineered antibodies is a promising strategy to reduce autoantibody levels in autoimmune diseases.
Maximilian Brinkhaus, Erwin Pannecoucke, Elvera J. van der Kooi, Arthur E. H. Bentlage, Ninotska I. L. Derksen, Julie Andries, Bianca Balbino, Magdalena Sips, Peter Ulrichts, Peter Verheesen, Hans de Haard, Theo Rispens, Savvas N. Savvides, Gestur Vidarsson   +13 more
doaj   +1 more source

Design of an alternate antibody fragment format that can be produced in the cytoplasm of Escherichia coli

Scientific Reports, 2023
With increased accessibility and tissue penetration, smaller antibody formats such as antibody fragments (Fab) and single chain variable fragments (scFv) show potential as effective and low-cost choices to full-length antibodies.
Aatir A. Tungekar, Lloyd W. Ruddock
doaj   +1 more source

Direct IgG epitope mapping on bacterial AB toxins by cryo-EM

STAR Protocols, 2021
Summary: This cryo-EM protocol was used to determine the B cell epitope map on the CdtB subunit of typhoid toxin, an A2B5 toxin secreted by Salmonella Typhi during infection.
Tri Nguyen, Jeongmin Song
doaj   +1 more source

First clinical experience with fractionated intracavitary radioimmunotherapy using [177Lu]Lu-6A10-Fab fragments in patients with glioblastoma: a pilot study

EJNMMI Research, 2023
Background Following resection and standard adjuvant radio- and chemotherapy, approved maintenance therapies for glioblastoma are lacking. Intracavitary radioimmunotherapy (iRIT) with 177Lu-labeled 6A10-Fab fragments targeting tumor-associated carbonic ...
Wolfgang Roll, Michael Müther, Guido Böning, Astrid Delker, Nils Warneke, Franz-Josef Gildehaus, Michael Schäfers, Walter Stummer, Reinhard Zeidler, Hans-Jürgen Reulen, Lars Stegger   +10 more
doaj   +1 more source

A Fab-Selective Immunoglobulin-Binding Domain from Streptococcal Protein G with Improved Half-Life Extension Properties. [PDF]

PLoS ONE, 2015
Half-life extension strategies have gained increasing interest to improve the pharmacokinetic and pharmacodynamic properties of protein therapeutics. Recently, we established an immunoglobulin-binding domain (IgBD) from streptococcal protein G (SpGC3) as
Felix Unverdorben, Meike Hutt, Oliver Seifert, Roland E Kontermann   +3 more
doaj   +1 more source

Cell-free synthesis of functional and endotoxin-free antibody Fab fragments by translocation into microsomes

BioTechniques, 2012
A eukaryotic cell-free system based on Spodoptera frugiperda cells was developed for the convenient synthesis of Fab antibody fragments and other disulfide bridge containing proteins.
Helmut Merk, Christine Gless, Barbara Maertens, Michael Gerrits, Wolfgang Stiege   +4 more
doaj   +1 more source

In silico antibody engineering for SARS-CoV-2 detection

Computational and Structural Biotechnology Journal, 2021
Engineered immunoglobulin-G molecules (IgGs) are of wide interest for the development of detection elements in protein-based biosensors with clinical applications.
Didac Martí, Eduard Martín-Martínez, Juan Torras, Oscar Bertran, Pau Turon, Carlos Alemán   +5 more
doaj   +1 more source

Purification of the Major Outer Membrane Protein of Azospirillum brasilense, Its Affinity to Plant Roots, and Its Involvement in Cell Aggregation

Molecular Plant-Microbe Interactions, 2001
The major outer membrane protein (MOMP) of the nitrogen-fixing rhizobacterium Azospirillum brasilense strain Cd was purified and isolated by gel filtration, and antiserum against this protein was obtained.
Saul Burdman, Gabriella Dulguerova, Yaacov Okon, Edouard Jurkevitch   +3 more
doaj   +1 more source