Results 21 to 30 of about 49,117 (237)

Purification of the Major Outer Membrane Protein of Azospirillum brasilense, Its Affinity to Plant Roots, and Its Involvement in Cell Aggregation

Molecular Plant-Microbe Interactions, 2001
The major outer membrane protein (MOMP) of the nitrogen-fixing rhizobacterium Azospirillum brasilense strain Cd was purified and isolated by gel filtration, and antiserum against this protein was obtained.
Saul Burdman, Gabriella Dulguerova, Yaacov Okon, Edouard Jurkevitch   +3 more
doaj   +1 more source

Platelets and lymphocytes drive progressive penumbral tissue loss during middle cerebral artery occlusion in mice

Journal of Neuroinflammation, 2021
Background In acute ischemic stroke, cessation of blood flow causes immediate tissue necrosis within the center of the ischemic brain region accompanied by functional failure in the surrounding brain tissue designated the penumbra.
Michael K. Schuhmann, Michael Bieber, Maximilian Franke, Alexander M. Kollikowski, David Stegner, Katrin G. Heinze, Bernhard Nieswandt, Mirko Pham, Guido Stoll   +8 more
doaj   +1 more source

A plug-and-play approach to antibody-based therapeutics via a chemoselective dual click strategy. [PDF]

, 2015
Although recent methods for the engineering of antibody-drug conjugates (ADCs) have gone some way to addressing the challenging issues of ADC construction, significant hurdles still remain.
Caddick, S, Chester, KA, Chudasama, V, Maruani, A, Miranda, E, Smith, ME   +5 more
core   +1 more source

Refinement of an ovine-based immunoglobulin therapy against SARS-CoV-2, with comparison of whole IgG versus F(ab′)2 fragments

Scientific Reports, 2023
The development of new therapies against SARS-CoV-2 is required to extend the toolkit of intervention strategies to combat the global pandemic. In this study, hyperimmune plasma from sheep immunised with whole spike SARS-CoV-2 recombinant protein has ...
Stephen Findlay-Wilson, Linda Easterbrook, Sandra Smith, Neville Pope, Matthew Aldridge, Gareth Humphries, Holger Schuhmann, Didier Ngabo, Emma Rayner, Ashley Otter, Thomas Coleman, Bethany Hicks, Rachel Halkerston, Kostis Apostolakis, Stephen Taylor, Susan Fotheringham, Amanda Horton, Irene CanoCejas, Matthew Wand, Julia A. Tree, Mark Sutton, Victoria Graham, Roger Hewson, Stuart Dowall   +23 more
doaj   +1 more source

Crystallization of immunoglobulin Fab fragments specific for DNA.

Journal of Biological Chemistry, 1988
The purification and crystallization of Fab fragments of two mouse monoclonal immunoglobulins specific for different DNA structures are described. In each case, papain digestion of the immunoglobulins produced a mixture of Fab species differing in their isoelectric points.
A, Boodhoo, C D, Mol, J S, Lee, W F, Anderson   +3 more
openaire   +2 more sources

Structural Basis for Enhanced HIV-1 Neutralization by a Dimeric Immunoglobulin G Form of the Glycan-Recognizing Antibody 2G12

Cell Reports, 2013
The human immunoglobulin G (IgG) 2G12 recognizes high-mannose carbohydrates on the HIV type 1 (HIV-1) envelope glycoprotein gp120. Its two antigen-binding fragments (Fabs) are intramolecularly domain exchanged, resulting in a rigid (Fab)2 unit including ...
Yunji Wu, Anthony P. West, Jr., Helen J. Kim, Matthew E. Thornton, Andrew B. Ward, Pamela J. Bjorkman   +5 more
doaj   +1 more source

Humanized CD30-Targeted Chimeric Antigen Receptor T Cells Exhibit Potent Preclinical Activity Against Hodgkin’s Lymphoma Cells

Frontiers in Cell and Developmental Biology, 2022
CD30-directed chimeric antigen receptors (CARs) with single chain antibody fragment (scFv)-binding domains from murine HRS3 show strong cytotoxicity to Hodgkin’s Lymphoma cells and have been used in clinical trials.
Jing Guo, Shuai He, Yongjie Zhu, Wei Yu, Dong Yang, Xudong Zhao   +5 more
doaj   +1 more source

Inhibition by small-molecule ligands of formation of amyloid fibrils of an immunoglobulin light chain variable domain. [PDF]

, 2015
Overproduction of immunoglobulin light chains leads to systemic amyloidosis, a lethal disease characterized by the formation of amyloid fibrils in patients' tissues. Excess light chains are in equilibrium between dimers and less stable monomers which can
Brumshtein, Boris, Cascio, Duilio, Eisenberg, David S, Esswein, Shannon R, Ly, Alan T, Phillips, Martin L, Salwinski, Lukasz, Sawaya, Michael R   +7 more
core   +1 more source

An empirical approach towards the efficient and optimal production of influenza-neutralizing ovine polyclonal antibodies demonstrates that the novel adjuvant CoVaccine HT(TM) is functionally superior to Freund's adjuvant [PDF]

, 2013
Passive immunotherapies utilising polyclonal antibodies could have a valuable role in preventing and treating infectious diseases such as influenza, particularly in pandemic situations but also in immunocompromised populations such as the elderly, the ...
A Kourti, AF Ochsenbein, AG Blom, AR Newcombe, C Cresswell, C Newcombe, Cara K. Fraser, CK Fraser, D Corti, DK Hong, E Klechevsky, FC Jensen, FW Santiago, G Jego, GA McMillin, H Ogonuki, H Ueno, J Aucouturier, J Guan, J Hinkula, J Thatte, J Ye, JA Turkstra, JC Cox, JD Berry, JE McElhaney, John D. Hayball, JT Wu, Kerrilyn R. Diener, L Sjostrom, L Vidard, LM Grant, M Baz, M Leenaars, M Mahdi Abdel Hamid, M Singh, MF Patria, Michael P. Brown, MJ Lynch, Mohammed Alsharifi, MR Buckwalter, Natalie E. Stevens, PR Dormitzer, R Bodewes, R Bodewes, RJ Scibienski, RM Redwan el, RM Zinkernagel, S Chhatre, S Martin-Gomez, SC de Cassan, SH van Ierssel, SJ Draper, T Suda, TT Wang, Valerie A. Ferro, W Luytjes, WC Ng, Y Mine, YT Chang   +59 more
core   +14 more sources

Crystallization and structure determination of an autoimmune anti-poly(dT) immunoglobulin Fab fragment at 3.0 A resolution.

Journal of Biological Chemistry, 1987
HED10 is an autoimmune antibody (IgG) which shows considerable specificity for the single-stranded DNA poly(dT). Production of Fab fragments by papain digestion resulted in heterogeneity as judged by isoelectric focusing gels, which had a marked negative
M. Cygler, A. Boodhoo, Jeremy S. Lee, W. Anderson   +3 more
semanticscholar   +1 more source