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Current Protocols, 2022
AbstractImmunohistochemistry is an essential technique for the localization and measurement of proteins in cells and tissues. This article describes methods for labeling proteins in adherent and suspension cell cultures and in tissue sections. Choices of antibodies and detection methods are discussed, and detailed troubleshooting guidelines are ...
Patricia A, Loughran +2 more
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AbstractImmunohistochemistry is an essential technique for the localization and measurement of proteins in cells and tissues. This article describes methods for labeling proteins in adherent and suspension cell cultures and in tissue sections. Choices of antibodies and detection methods are discussed, and detailed troubleshooting guidelines are ...
Patricia A, Loughran +2 more
openaire +2 more sources
Current Protocols in Molecular Biology, 2008
AbstractImmunohistochemistry is a vastly diverse and essential method for localization of proteins in cells and tissues. This unit presents methods for labeling proteins in suspension and adherent cultures and in tissue sections, using detection methods for both fluorescence and brightâfield microscopy.
Simon C. Watkins, Marsha Goldstein
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AbstractImmunohistochemistry is a vastly diverse and essential method for localization of proteins in cells and tissues. This unit presents methods for labeling proteins in suspension and adherent cultures and in tissue sections, using detection methods for both fluorescence and brightâfield microscopy.
Simon C. Watkins, Marsha Goldstein
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Current Protocols in Immunology, 2013
AbstractThis unit describes several methods for localizing specific antigens in various tissue and cell preparations using immunohistochemistry (IHC). Protocols describe preparation of suitable material for IHC including fresh, unfixed, frozen tissue specimens; unfixed cells, either freshly isolated or derived from suspension or adherent cultures; or ...
Clive R. Taylor, Florence M. Hofman
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AbstractThis unit describes several methods for localizing specific antigens in various tissue and cell preparations using immunohistochemistry (IHC). Protocols describe preparation of suitable material for IHC including fresh, unfixed, frozen tissue specimens; unfixed cells, either freshly isolated or derived from suspension or adherent cultures; or ...
Clive R. Taylor, Florence M. Hofman
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Immunohistochemistry of Otosclerosis
Acta Oto-Laryngologica, 1990Immunoglobulins G (IgG) and A (IgA) were found in plasma cells, osteocytes, chondrocytes and in connective tissue of active otosclerotic (= otospongiotic) lesions. The application of antibodies against paramyxovirus and rubella virus antigens reproducibly determined the expression of these antigens at different sites in the otosclerotic stapes, but ...
Wolfgang Arnold, Imre Friedmann
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