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Fluorescent Immunohistochemistry
2021Immunofluorescence is an important immunochemical technique that utilizes fluorescence-labeled antibodies to detect specific target antigens. It is used widely in both scientific research and clinical laboratories. Immunofluorescence allows for excellent sensitivity and amplification of signal in comparison to immunohistochemistry. However, analysis of
Vanessa, Moreno+2 more
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Immunohistochemistry of retinoblastomas
Journal of Neuro-Oncology, 1987Immunohistochemical stains using neuronal and glial marker proteins were applied to retinoblastomas tissues from 14 children. Among the neurofilament triplet proteins, NF68Kd positive cells were observed in 12. Few NF160Kd positive cells were noted in 2, and NF210Kd positive cells were not detected.
Inomata H+4 more
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Immunocytochemistry and Immunohistochemistry
2003Immunohistochemistry allows the specific histochemical localization of many diverse classes of organic substances, including inflammatory markers ranging from biogenic amines to macromolecular cytokines and their receptors. Corresponding ligands and their receptors can be demonstrated simultaneously by double-labeling protocols (1), since the fixation ...
Springer, J, Fischer, A
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Standardization in Immunohistochemistry
Applied Immunohistochemistry & Molecular Morphology, 2001Over the last few years, the role of immunohistochemical analysis has been changing from that of an ancillary diagnostic technique to that of a stand-alone diagnostic method, the results of which determine treatment approach. With this change comes a need for increasing standardization of both preanalytical and analytical methods, so that results ...
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Immunohistochemistry Microarrays
Analytical Chemistry, 2017Immunohistochemistry (IHC) on tissue sections is widely used for quantifying the expression patterns of proteins and is part of the standard of care for cancer diagnosis and prognosis, but is limited to staining a single protein per tissue. Tissue microarray and microfluidics staining methods have emerged as powerful high throughput techniques, but ...
Huiyan Li+5 more
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Enzymatic Immunohistochemistry
2021Immunohistochemistry (IHC) is a powerful laboratory technique that is widely used in a variety of settings, including clinical medicine and research. Enzymatic immunohistochemistry refers to the process of detecting cell surface proteins or antigens using enzyme-labeled antibodies.
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Multiplex Immunohistochemistry and Immunofluorescence: A Practical Update for Pathologists.
Modern Pathology, 2023P. Harms+8 more
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Immunohistochemistry in gynecological pathology
International Journal of Gynecological Cancer, 2003Although the majority of diagnoses in gynecological pathology are established on examination of routine hematoxylin and eosin stained sections, additional tests are occasionally required. Immunohistochemistry is widely used to provide additional diagnostic information in problematic cases.
Anais Malpica+2 more
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Immunohistochemistry of Cancers
2014This chapter provides an overview of mostly malignant tumors of various organs with focus on the most common types. The authors have used their own experience in addition to literature review and have discussed the most common and useful immunohistochemistry markers in the diagnosis and differential diagnosis of various malignant tumors.
Issa Jahanzad+2 more
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Principles and Methods of Immunohistochemistry
2010Immunohistochemical techniques detect antigens in tissue sections by means of immunological and chemical reactions. This technique is highly sensitive and specific and can detect a wide variety of antigens in multiple animal species. This chapter reviews common immunohistochemical methods used in the characterization of normal and pathologic tissue and
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