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Immunophenotyping of Plasma Cells
Current Protocols in Cytometry, 2006AbstractPlasma cell enumeration and immunophenotyping has been shown to be of value in predicting the outcome for patients with myeloma. Detection of abnormal plasma cells at a leukocyte level >0.01% at the end of therapy predicts early relapse. Although clonally related populations at varying stages of differentiation are reported to be present in ...
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Immunophenotyping as a guide for targeted therapy
Best Practice & Research Clinical Haematology, 2003Immunophenotyping of acute and chronic leukaemias has revealed many lineage- and differentiation-specific antigens. It has now become possible to classify leukaemias according to their unique antigenic expression pattern. Among many lineage- and differentiation-specific antigens, disease-specific antigens are increasingly recognized because of their ...
Pieter Sonneveld, Rob Pieters
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2004
Publisher Summary B-lymphocytes provide the humoral (antibody-mediated) arm of the adaptive immune system. Like all leukocytes, B cells develop from hematopoietic stem cells in the bone marrow. The bone marrow is a tissue where B cells of all developmental stages can be isolated.
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Publisher Summary B-lymphocytes provide the humoral (antibody-mediated) arm of the adaptive immune system. Like all leukocytes, B cells develop from hematopoietic stem cells in the bone marrow. The bone marrow is a tissue where B cells of all developmental stages can be isolated.
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Immunophenotyping by Mass Cytometry
2019Mass cytometry is a novel technology similar to flow cytometry in which antibodies are tagged with heavy metal molecules rather than fluorophores and then detected with time-of-flight mass spectrometry. This enables measurement of up to 50 simultaneous parameters with no autofluorescent background and little or no spillover or required compensation ...
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Immunophenotyping of male breast cancer
Histopathology, 2012Kornegoor R, Verschuur‐Maes A H J, Buerger H, Hogenes M C, de Bruin P C, Oudejans J J, Hinrichs B & van Diest P J (2012) Histopathology Immunophenotyping of male breast cancerAims: Male breast cancer is a rare disease, and knowledge of carcinogenesis is limited.
Bernd Hinrichs +7 more
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Mycosis fungoides with a CD56+ immunophenotype
Journal of the American Academy of Dermatology, 2005We report 3 cases of mycosis fungoides (MF) with a CD56+ cytotoxic immunophenotype. Each patient presented with a different clinical phenotype: one exhibited limited poikilodermatous patches (skin stage T1); one, widespread hypopigmented lesions (skin stage T2); and one, poikiloderma with a single cutaneous tumor (skin stage T3).
Guy Orchard +5 more
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Fluorescent Cell Barcoding for Immunophenotyping
2019Immunophenotyping using flow cytometry highly benefits from multiplexing samples for generation of more robust data, because of reduction of antibody consumption, batch effect and technical variations. One way to multiplex is via fluorescent cell barcoding (FCB) prior to staining procedure.FCB is a high-throughput multiplexed assay using various ...
Giudice V., Fantoni G., Biancotto A.
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Immunophenotypic Analysis of Acute Leukemia
American Journal of Clinical Pathology, 1993Since the introduction of monoclonal antibodies for the study of hematopoietic disease in the early 1980s, techniques to aid in the identification of cell lineage have proliferated. Immunofluorescent flow-cytometric studies are particularly helpful in the characterization of acute leukemia, and when used in conjunction with light-microscopic findings ...
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Immunophenotyping in Myelodysplastic Syndromes
2013Myelodysplastic syndromes (MDS) comprise a heterogeneous group of clonal hematological disorders that is diagnosed cytomorphologically. They are characterized by ineffective hematopoiesis resulting in peripheral blood cytopenias and showing by definition in all cases dysplastic features in at least one lineage in the bone marrow, an increased myeloid ...
Arjan A. van de Loosdrecht +1 more
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Cytogenetics and Immunophenotype
Leukemia & Lymphoma, 1994Herman van den Berghe, Christina Mecucci
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