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Current Protocols in Cytometry, 1997
AbstractThis unit presents basic techniques for immunophenotyping by flow cytometry, direct using a conjugated monoclonal antibody and indirect using an unconjugated primary antibody followed by a conjugated secondary antibody. Combinations of these methods are described for two‐, three‐, and four‐color staining.
C C, Stewart, S J, Stewart
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AbstractThis unit presents basic techniques for immunophenotyping by flow cytometry, direct using a conjugated monoclonal antibody and indirect using an unconjugated primary antibody followed by a conjugated secondary antibody. Combinations of these methods are described for two‐, three‐, and four‐color staining.
C C, Stewart, S J, Stewart
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Seminars in Hematology, 2001
Immunophenotyping of leukocytes for nonmalignant conditions uses the power of multiparameter flow cytometry to count specific lymphocyte populations and evaluate the presence or absence of particular cell surface markers. Its main clinical indications, and the focus of this chapter, are enumeration of CD4 T-cell counts and activation markers on T cells
J J, Bleesing, T A, Fleisher
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Immunophenotyping of leukocytes for nonmalignant conditions uses the power of multiparameter flow cytometry to count specific lymphocyte populations and evaluate the presence or absence of particular cell surface markers. Its main clinical indications, and the focus of this chapter, are enumeration of CD4 T-cell counts and activation markers on T cells
J J, Bleesing, T A, Fleisher
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Journal of Immunological Methods, 2000
Modern immunophenotyping of hematological malignancies by flow cytometry is assisted by a wide array of easily accessible monoclonal antibodies, by antibodies conjugated to diverse fluorochromes, and by reliable techniques for cell membrane permeabilization.
D, Campana, F G, Behm
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Modern immunophenotyping of hematological malignancies by flow cytometry is assisted by a wide array of easily accessible monoclonal antibodies, by antibodies conjugated to diverse fluorochromes, and by reliable techniques for cell membrane permeabilization.
D, Campana, F G, Behm
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The Immunophenotype of Ependymomas
Applied Immunohistochemistry & Molecular Morphology, 2000The morphologic distinction of ependymomas with epithelial cytology from metastatic carcinoma may pose a significant problem in differential diagnosis. The known presence of keratin in glioma cells further complicates the issue. Using the labeled streptavidin-biotin method with automated staining, we studied epithelial and glial marker expression in 52
K D, Vege, C, Giannini, B W, Scheithauer
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Immunophenotype of Pleomorphic Xanthoastrocytoma
The American Journal of Surgical Pathology, 1998Pleomorphic xanthoastrocytoma (PXA) is an uncommon tumor, often seizure-associated and occurring in the temporal lobe of young adults. Although its cells are considered astrocytic in nature, recent studies suggest the presence of neuronal differentiation and a possible relationship to glioneuronal neoplasms.
Giannini, C +5 more
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A Practical Cryopreservation and Staining Protocol for Immunophenotyping in Population Studies [PDF]
Current Protocols in Cytometry, 2018 Large population‐based cohort studies, through their prospective collection of a broad range of health information, represent an invaluable resource for novel insights into the pathogenesis of human diseases.
Hélène Barcelo +2 more
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Immunophenotyping of normal lymphocytes
Clinical & Laboratory Haematology, 2008We report the data of direct and indirect immunofluorescence labelling of peripheral blood mononuclear cells obtained from 40 normal controls and nine patients having blood tests for non-haematological disorders (PNHD controls) using flow cytometric analysis.
R E, Peters, S, al-Ismail
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Immunophenotypic Analysis of Platelets
Current Protocols in Cytometry, 2002AbstractPlatelets are the smallest cellular component in the peripheral circulation. Their primary role is maintenance of hemostasis. The evaluation of platelets by flow cytometry has proven beneficial in the investigation of many disease states, including inherited defects, cardiovascular disease, stroke, and many other inflammatory processes.
Lori A, Krueger +4 more
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Immunophenotyping of Paucicellular Samples
Current Protocols in Cytometry, 2014ABSTRACTImmunophenotyping of paucicellular samples may represent a diagnostic challenge in the flow cytometry (FC) laboratory routine, as the scarcity of cells limits the number of tests that can be performed. Specimens such as fine needle aspirates (FNA), human body fluids (BF), cerebrospinal fluid (CSF), or ocular fluid (OF) sent for FC ...
Alessandra, Stacchini +2 more
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Immunophenotypic heterogeneity in osteosarcomas
Human Pathology, 1991Eighteen osteosarcomas were studied immunohistochemically. The tumors were classified into the following six histologic subtypes: five osteoblastic, four chondroblastic, four malignant fibrous histiocytoma-like, two telangiectatic, two low-grade central, and one giant cell-rich. Variable amounts of osteocalcin immunoreactivity were found in all tumors.
T, Hasegawa +5 more
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