Results 291 to 300 of about 134,678 (321)
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Detection of Genetic Variants of Haemophilia B with an Immunosorbent Technique
British Journal of Haematology, 1973Summary. With an immunosorbent technique, we have found multiple genetic variants of haemophilia B. In one variant there is no detectable material that cross‐reacts with a human anti‐factor‐IX antibody. This variant is defined as negative for cross‐reacting material (CRM−).
W. R. Neal +3 more
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Enzyme-Linked Immunosorbent Assay (ELISA) Technique for Food Analysis
2021Enzyme-linked immunosorbent assay (ELISA) techniques employ a highly sensitive and specific form of immunological reactions, and this technique shows wide applications in food analysis. The versatility functions of ELISA techniques render them suitable to detect specific constituents in food, including the natural components, pesticide, therapeutic ...
Zheng Feei Ma +2 more
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Ultrasound wave-mediated enzyme-linked immunosorbent assay technique
Analytica Chimica Acta, 2009With the increasing burden of infectious diseases, it has become important to develop a rapid ELISA that could facilitate early diagnosis. Herein, we have shown that ultrasound waves can dramatically reduce the ELISA timing without losing its specificity or sensitivity.
Pragya Sharma, Pradip Nahar
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Enzyme-linked immunosorbent assays and developments in techniques using latex beads
Current Opinion in Immunology, 1991Not surprisingly, most of the publications on enzyme immunoassays or latex agglutination tests in 1990 have been applications for specific antigens or antibodies. Nevertheless, a large number of studies of considerable interest to all users have been reported.
Albert Adam, Peter Tijssen
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Application of the Partial Least Square Technique to Identify Critical Variables in the Immunosorbent Manufacturing [PDF]
Chromatographia, 2008 The partial least square technique (PLS) was applied to the monoclonal antibody (Mab) CB.Hep-1 immunosorbent manufacturing to determine the influence of cyanate ester concentration, ligand concentration and target ligand density on Mab coupling efficiency, elution capacity, Hepatitis B surface antigen purity and ligand leakage (output variables ...
Kenia M. Sánchez +16 more
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Techniques and instrumentation for preparative immunosorbent separations
Journal of Chromatography A, 1978Abstract Studies of the physical and chemical characteristics of immunosorbents have allowed operational improvements to be made in their application to preparative separation of pure antigens and monospecific antibodies. The loss of immunochemical reactivity with increasing protein substitution and the limitations imposed by the porous nature of ...
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Enzyme-linked immunosorbent assay (ELISA) for HIV antibody by a glass slide technique
Journal of Immunological Methods, 1991An enzyme-linked immunosorbent assay (ELISA) technique is described which utilizes a commercially available glass microscope slide coated with hydrophobic teflon in such a pattern as to give 30 small circular wells, each of which has a glass bottom.
M. Jane Ludwig +3 more
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Vox Sanguinis, 1983
Abstract. A solid‐phase kinetic enzyme‐linked immunosorbent assay has been developed to measure binding of antibodies to purified or synthetic blood group antigens and tested in the Lewis blood group system. Chemically synthesized Lewis a antigen is used as the target for the binding of serum antibody in this sandwich‐type assay.
J. T. Holt +5 more
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Abstract. A solid‐phase kinetic enzyme‐linked immunosorbent assay has been developed to measure binding of antibodies to purified or synthetic blood group antigens and tested in the Lewis blood group system. Chemically synthesized Lewis a antigen is used as the target for the binding of serum antibody in this sandwich‐type assay.
J. T. Holt +5 more
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The Analyst, 2014
The detection limit is one of the most important performance parameters for bioanalytical techniques. Here we present a generic method to estimate the detection limit of biomolecular assays based on a step-by-step analysis of the assay procedure. Enzyme-linked immunosorbent assay (ELISA) is used here as an example; however, much of the information ...
Zhang, Shiyun +3 more
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The detection limit is one of the most important performance parameters for bioanalytical techniques. Here we present a generic method to estimate the detection limit of biomolecular assays based on a step-by-step analysis of the assay procedure. Enzyme-linked immunosorbent assay (ELISA) is used here as an example; however, much of the information ...
Zhang, Shiyun +3 more
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Journal of Immunological Methods, 1994
This study describes the use of electrophoretically purified antigens blotted onto nitrocellulose, as solid phase antigens for enzyme-linked immunosorbent assays. This procedure is called DMISA, for dissociated membrane immunosorbent assay. The method is illustrated using immunoblotted antigens of Dactylis glomerata grass pollen extract.
H. Sénéchal +6 more
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This study describes the use of electrophoretically purified antigens blotted onto nitrocellulose, as solid phase antigens for enzyme-linked immunosorbent assays. This procedure is called DMISA, for dissociated membrane immunosorbent assay. The method is illustrated using immunoblotted antigens of Dactylis glomerata grass pollen extract.
H. Sénéchal +6 more
openaire +3 more sources