Results 301 to 310 of about 145,189 (327)
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Detection of Genetic Variants of Haemophilia B with an Immunosorbent Technique
British Journal of Haematology, 1973Summary. With an immunosorbent technique, we have found multiple genetic variants of haemophilia B. In one variant there is no detectable material that cross‐reacts with a human anti‐factor‐IX antibody. This variant is defined as negative for cross‐reacting material (CRM−).
W R, Neal +3 more
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Techniques and instrumentation for preparative immunosorbent separations
Journal of Chromatography A, 1978Abstract Studies of the physical and chemical characteristics of immunosorbents have allowed operational improvements to be made in their application to preparative separation of pure antigens and monospecific antibodies. The loss of immunochemical reactivity with increasing protein substitution and the limitations imposed by the porous nature of ...
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Vox Sanguinis, 1983
Abstract. A solid‐phase kinetic enzyme‐linked immunosorbent assay has been developed to measure binding of antibodies to purified or synthetic blood group antigens and tested in the Lewis blood group system. Chemically synthesized Lewis a antigen is used as the target for the binding of serum antibody in this sandwich‐type assay.
S, Spitalnik +5 more
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Abstract. A solid‐phase kinetic enzyme‐linked immunosorbent assay has been developed to measure binding of antibodies to purified or synthetic blood group antigens and tested in the Lewis blood group system. Chemically synthesized Lewis a antigen is used as the target for the binding of serum antibody in this sandwich‐type assay.
S, Spitalnik +5 more
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Journal of Immunological Methods, 1994
This study describes the use of electrophoretically purified antigens blotted onto nitrocellulose, as solid phase antigens for enzyme-linked immunosorbent assays. This procedure is called DMISA, for dissociated membrane immunosorbent assay. The method is illustrated using immunoblotted antigens of Dactylis glomerata grass pollen extract.
C, Guérin-Marchand +6 more
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This study describes the use of electrophoretically purified antigens blotted onto nitrocellulose, as solid phase antigens for enzyme-linked immunosorbent assays. This procedure is called DMISA, for dissociated membrane immunosorbent assay. The method is illustrated using immunoblotted antigens of Dactylis glomerata grass pollen extract.
C, Guérin-Marchand +6 more
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Enzyme-linked immunosorbent assay (ELISA) for HIV antibody by a glass slide technique
Journal of Immunological Methods, 1991An enzyme-linked immunosorbent assay (ELISA) technique is described which utilizes a commercially available glass microscope slide coated with hydrophobic teflon in such a pattern as to give 30 small circular wells, each of which has a glass bottom.
K O, Smith +3 more
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Analytical Biochemistry, 1991
PCR products obtained using primers carrying at their 5' ends biotin and an antigenic group (e.g., fluorescein) can be quantitatively analyzed by immunological techniques. The procedure described here does not require electrophoretic separation and/or hybridization with radioactive probes.
A, Landgraf, B, Reckmann, A, Pingoud
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PCR products obtained using primers carrying at their 5' ends biotin and an antigenic group (e.g., fluorescein) can be quantitatively analyzed by immunological techniques. The procedure described here does not require electrophoretic separation and/or hybridization with radioactive probes.
A, Landgraf, B, Reckmann, A, Pingoud
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The Analyst, 2014
The detection limit is one of the most important performance parameters for bioanalytical techniques. Here we present a generic method to estimate the detection limit of biomolecular assays based on a step-by-step analysis of the assay procedure. Enzyme-linked immunosorbent assay (ELISA) is used here as an example; however, much of the information ...
Zhang, Shiyun +3 more
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The detection limit is one of the most important performance parameters for bioanalytical techniques. Here we present a generic method to estimate the detection limit of biomolecular assays based on a step-by-step analysis of the assay procedure. Enzyme-linked immunosorbent assay (ELISA) is used here as an example; however, much of the information ...
Zhang, Shiyun +3 more
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American Journal of Veterinary Research, 1986
SUMMARY A new sandwich enzyme-linked immunosorbent assay, using monoclonal and polyclonal antibodies, was developed to detect transmissible gastroenteritis virus antigens from cell culture and from intestinal wash or feces obtained from experimentally infected pigs.
S, Bernard +3 more
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SUMMARY A new sandwich enzyme-linked immunosorbent assay, using monoclonal and polyclonal antibodies, was developed to detect transmissible gastroenteritis virus antigens from cell culture and from intestinal wash or feces obtained from experimentally infected pigs.
S, Bernard +3 more
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American Journal of Veterinary Research, 1985
SUMMARY A microenzyme-linked immunosorbent assay (dot-elisa) was modified for making an immunodiagnosis of Fasciola hepatica infections in sheep. Sheep were alloted as follows: group I—3 controls and 4 principals, each inoculated with 500 metacercariae; group II—3 controls and 7 principals, each inoculated with 250 metacercariae; and group III—3 ...
G L, Zimmerman, M J, Nelson, C R, Clark
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SUMMARY A microenzyme-linked immunosorbent assay (dot-elisa) was modified for making an immunodiagnosis of Fasciola hepatica infections in sheep. Sheep were alloted as follows: group I—3 controls and 4 principals, each inoculated with 500 metacercariae; group II—3 controls and 7 principals, each inoculated with 250 metacercariae; and group III—3 ...
G L, Zimmerman, M J, Nelson, C R, Clark
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Journal of Chromatography A, 1998
An enzyme-linked immunosorbent assay (ELISA) on microplates and a HPLC coupled-column switching method were compared for the determination of the nitroarene compound 1-nitropyrene in airborne particulate organic matter collected at a busy intersection over a period of 2 months.
J, Zühlke, D, Knopp, R, Niessner
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An enzyme-linked immunosorbent assay (ELISA) on microplates and a HPLC coupled-column switching method were compared for the determination of the nitroarene compound 1-nitropyrene in airborne particulate organic matter collected at a busy intersection over a period of 2 months.
J, Zühlke, D, Knopp, R, Niessner
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