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Xenoperfusion-The Transformative Role of Machine Perfusion in Xenotransplantation. [PDF]
XenotransplantationHuwyler F +3 more
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2003
In situhybridization permits specific identification of genes and gene expression without removing the target sequence from its topographical surroundings. The technique was described simultaneously in 1960 by two groups (1,2). It depends on the hybridization of a labeled nucleic acid probe to a complementary sequence of tissue mRNA or DNA.
C G, Beckett, P J, Ciclitira
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In situhybridization permits specific identification of genes and gene expression without removing the target sequence from its topographical surroundings. The technique was described simultaneously in 1960 by two groups (1,2). It depends on the hybridization of a labeled nucleic acid probe to a complementary sequence of tissue mRNA or DNA.
C G, Beckett, P J, Ciclitira
+12 more sources
2022
In situ HiC uses the relative frequency of DNA-DNA ligation events to reconstruct the three-dimensional architecture of a genome. As such, restriction enzyme digested ends of genomic DNA within fixed nuclei are tagged with biotinylated dNTPs. DNA-DNA ligation events generated via proximity ligation are then captured, amplified and next generation ...
Timothy M, Johanson, Rhys S, Allan
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In situ HiC uses the relative frequency of DNA-DNA ligation events to reconstruct the three-dimensional architecture of a genome. As such, restriction enzyme digested ends of genomic DNA within fixed nuclei are tagged with biotinylated dNTPs. DNA-DNA ligation events generated via proximity ligation are then captured, amplified and next generation ...
Timothy M, Johanson, Rhys S, Allan
openaire +2 more sources
2013
A non-radioactive in situ hybridization protocol is presented for localization of mRNA transcripts in thin tissue sections. In situ hybridization provides spatial resolution of transcript distribution at the cellular level that is not attained by Northern hybridization or PCR-based methods using organ- or tissue-derived RNA. With appropriately designed
Chung-Jui, Tsai, Scott A, Harding
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A non-radioactive in situ hybridization protocol is presented for localization of mRNA transcripts in thin tissue sections. In situ hybridization provides spatial resolution of transcript distribution at the cellular level that is not attained by Northern hybridization or PCR-based methods using organ- or tissue-derived RNA. With appropriately designed
Chung-Jui, Tsai, Scott A, Harding
openaire +2 more sources
Nature Methods, 2007
Research performed where epidemics hit the hardest is necessary to bring solutions to the major health crises that plague poverty-stricken areas. Far from being limited to these areas, 'research in situ' can benefit health management worldwide. There are pressing technological needs to be addressed in order to facilitate such research.
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Research performed where epidemics hit the hardest is necessary to bring solutions to the major health crises that plague poverty-stricken areas. Far from being limited to these areas, 'research in situ' can benefit health management worldwide. There are pressing technological needs to be addressed in order to facilitate such research.
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1992
Soon after the method of in situ hybridization (ISH) had been published (Pardue and Gall, 1969), reports appeared showing that it could also be used with great success in the study of virus-infected systems (Orth et al., 1970; Geukens and May, 1974). Now it was possible to study the biology of viruses and the mechanisms of viral infections in detail ...
Genersch, E. +26 more
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Soon after the method of in situ hybridization (ISH) had been published (Pardue and Gall, 1969), reports appeared showing that it could also be used with great success in the study of virus-infected systems (Orth et al., 1970; Geukens and May, 1974). Now it was possible to study the biology of viruses and the mechanisms of viral infections in detail ...
Genersch, E. +26 more
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Journal of Cataract and Refractive Surgery, 1990
A new technique of phacoemulsification is described in which the nucleus is fractured within the capsular bag (in situ). The characteristics of this technique include hydrodissection, division of the nucleus into quadrants, and manipulation of each quadrant into the safe central zone for emulsification.
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A new technique of phacoemulsification is described in which the nucleus is fractured within the capsular bag (in situ). The characteristics of this technique include hydrodissection, division of the nucleus into quadrants, and manipulation of each quadrant into the safe central zone for emulsification.
openaire +2 more sources
Situs at the mirror: from situs inversus to situs ambiguus
2013Learning objectives: -Define the three types of situs (solitus, inversus and ambiguus or heterotaxy), approaching their anatomic features and related terminology in a comprehensible way.
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