Comparative evaluation of DNA and RNA probes for capture-based mitochondrial DNA next-generation sequencing [PDF]
BMC BiologyBackground Probe-based liquid-phase hybridization capture is a powerful and commonly used approach for next-generation sequencing (NGS) of mitochondrial DNA (mtDNA).
Tianlei Sun +8 more
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Enzymatic synthesis of reactive RNA probes containing squaramate-linked cytidine or adenosine for bioconjugations and cross-linking with lysine-containing peptides and proteins [PDF]
Communications ChemistryProtein-RNA interactions play important biological roles and hence reactive RNA probes for cross-linking with proteins are important tools in their identification and study.
Ivana Ivancová +7 more
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A PCR-Based Method for RNA Probes and Applications in Neuroscience [PDF]
Frontiers in Neuroscience, 2018 In situ hybridization (ISH) is a powerful technique that is used to detect the localization of specific nucleic acid sequences for understanding the organization, regulation, and function of genes.
Ruifang Hua +6 more
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Enriching Bacteria-Specific RNA From Host Samples Before NGS With Transcript-Capture [PDF]
Bio-ProtocolPathogen gene expression from host samples is often challenging to study due to low signal and high host RNA background. PCR probes have been recently used to hybridize and extract bacterial sequences from next-generation sequencing (NGS) libraries ...
Eleanor Lamont +4 more
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Post-transcriptional labeling by using Suzuki–Miyaura cross-coupling generates functional RNA probes [PDF]
Nucleic Acids Research, 2018 Seergazhi Srivatsan, Arun Tanpure
exaly +2 more sources
Molecules, 2022 RNA imaging is of great importance for understanding its complex spatiotemporal dynamics and cellular functions. Considerable effort has been devoted to the development of small-molecule fluorescent probes for RNA imaging.
Lan Fang +8 more
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Identification of soybean trans-factors associated with plastid RNA editing sites [PDF]
Genetics and Molecular Biology, 2020 RNA editing is a posttranscriptional process that changes nucleotide sequences, among which cytosine-to-uracil by a deamination reaction can revert non-neutral codon mutations.
Nureyev F. Rodrigues +3 more
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Sets of RNA repeated tags and hybridization-sensitive fluorescent probes for distinct images of RNA in a living cell. [PDF]
PLoS ONE, 2010 BACKGROUND: Imaging the behavior of RNA in a living cell is a powerful means for understanding RNA functions and acquiring spatiotemporal information in a single cell.
Takeshi Kubota +4 more
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Quantification of virus-infected cells using RNA FISH-Flow
STAR Protocols, 2023 Summary: We present a protocol to detect cells that have been infected by RNA viruses. The method, RNA fluorescence in situ hybridization flow cytometry (RNA FISH-Flow), uses 48 fluorescently labeled DNA probes that hybridize in tandem to viral RNA.
Cody J. Warren +6 more
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Fe.bleomycin as a probe of RNA conformation [PDF]
Nucleic Acids Research, 1996 Two crystallographically defined tRNAs, yeast tRNAAsp and tRNAPhe, were used as substrates for oxidative cleavage by Fe.bleomycin to facilitate definition at high resolution of the structural elements in RNAs conducive to bleomycin binding and cleavage. Yeast tRNAAsp underwent cleavage at G45 and U66; yeast tRNAPhe was cleaved at four sites, namely G19,
Holmes, Chris +4 more
openaire +3 more sources