Results 1 to 10 of about 169 (169)

Fe.bleomycin as a probe of RNA conformation [PDF]

Nucleic Acids Research, 1996
Two crystallographically defined tRNAs, yeast tRNAAsp and tRNAPhe, were used as substrates for oxidative cleavage by Fe.bleomycin to facilitate definition at high resolution of the structural elements in RNAs conducive to bleomycin binding and cleavage. Yeast tRNAAsp underwent cleavage at G45 and U66; yeast tRNAPhe was cleaved at four sites, namely G19,
C E, Holmes, A T, Abraham, S M, Hecht, C, Florentz, R, Giegé   +4 more
openaire   +2 more sources

Probing the structure of RNAs in solution [PDF]

Nucleic Acids Research, 1987
During these last years, a powerful methodology has been developed to study the secondary and tertiary structure of RNA molecules either free or engaged in complex with proteins. This method allows to test the reactivity of every nucleotide towards chemical or enzymatic probes.
C, Ehresmann, F, Baudin, M, Mougel, P, Romby, J P, Ebel, B, Ehresmann   +5 more
openaire   +2 more sources

Metabolic RNA labeling for probing RNA dynamics in bacteria [PDF]

Nucleic Acids Research, 2020
Abstract Metabolic labeling of RNAs with noncanonical nucleosides that are chemically active, followed by chemoselective conjugation with imaging probes or enrichment tags, has emerged as a powerful method for studying RNA transcription and degradation in eukaryotes.
Liying Meng, Yilan Guo, Qi Tang, Rongbing Huang, Yuchen Xie, Xing Chen   +5 more
openaire   +2 more sources

Probing RNA Folding Pathways by RNA Fingerprinting [PDF]

Current Protocols in Nucleic Acid Chemistry, 2000
AbstractThis unit provides protocols for using native polyacrylamide gel electrophoresis to distinguish folding and unfolding conformers of RNA. It is useful for studying conformers that can exchange in a period of minutes or seconds, and that are thus difficult to study by solution‐based methods.
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Probing the phenomics of noncoding RNA [PDF]

eLife, 2013
Genetic knockout experiments on mice confirm that some long noncoding RNA molecules have developmental functions.
openaire   +3 more sources

Probing RNA structure in vivo

Current Opinion in Structural Biology, 2019
RNA structure underpins many essential functions in biology. New chemical reagents and techniques for probing RNA structure in living cells have emerged in recent years. High-throughput, genome-wide techniques such as Structure-seq2 and DMS-MaPseq exploit nucleobase modification by dimethylsulfate (DMS) to obtain complete structuromes, and are ...
David, Mitchell, Sarah M, Assmann, Philip C, Bevilacqua   +2 more
openaire   +3 more sources

Osmium tetroxide as a probe of RNA structure [PDF]

RNA, 2017
Structured RNAs have a central role in cellular function. The capability of structured RNAs to adopt fixed architectural structures or undergo dynamic conformational changes contributes to their diverse role in the regulation of gene expression. Although numerous biophysical and biochemical tools have been developed to study structured RNAs, there is a
Zhang, Jing, Li, Danbin, Zhang, Jun, Chen, Dongrong, Murchie, Alastair I.H.   +4 more
openaire   +2 more sources

Fluorogenic Covalent Probes for RNA

Journal of the American Chemical Society
ABSTRACT Sequence-generalized fluorescent labels and stains for RNA can enable imaging, tracking and analysis of the biopolymer. However, current non-covalent RNA dyes are poorly selective for RNA over DNA, interact weakly with their target, and can show limited utility in cellular RNA staining due to poor selectivity and high ...
Jinwoo Shin, Moon Jung Kim, Eric T. Kool
openaire   +4 more sources

Claudin‐6 Protein Expression in Atypical Teratoid/Rhabdoid Tumors Is Strongly Enriched in the Molecular Subgroup AT/RT‐TYR

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Claudin‐6 has emerged as a promising immunotherapeutic target, yet protein‐level data in atypical teratoid/rhabdoid tumors (AT/RTs) have been inconsistent. We analyzed 36 well‐characterized AT/RT samples and found membranous claudin‐6 protein expression in 58% of cases, with striking enrichment in the molecular subgroup AT/RT‐TYR (100%) and ...
Victoria E. Fincke, Christian Thomas, Michael C. Frühwald, Pascal D. Johann, Martin Hasselblatt   +4 more
wiley   +1 more source

Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid‐derived monolayers compared to HeLa cells

FEBS Letters, EarlyView.
Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi, Pilar Samperio Ventayol, Natalie Burkard, Nicolas Schlegel, Carmen Aguilar, Sina Bartfeld   +5 more
wiley   +1 more source