Results 11 to 20 of about 392,196 (263)
Probing the structure of RNAs in solution [PDF]
Nucleic Acids Research, 1987 During these last years, a powerful methodology has been developed to study the secondary and tertiary structure of RNA molecules either free or engaged in complex with proteins. This method allows to test the reactivity of every nucleotide towards chemical or enzymatic probes.
Ehresmann, Chantal +5 more
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Highly Sensitive and Multiplexed In Situ RNA Profiling with Cleavable Fluorescent Tyramide
Cells, 2021 Understanding the composition, regulation, and function of complex biological systems requires tools that quantify multiple transcripts at their native cellular locations.
Lu Xiao, Joshua Labaer, Jia Guo
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Sensitive visualization of SARS-CoV-2 RNA with CoronaFISH
Life Science Alliance, 2022 We design an RNA-FISH method to target the plus and minus strands of SARS-CoV-2 RNA, and apply it to image the virus in cell lines, lung tissue, and nasal swabs, by fluorescence and electron microscopy.
Elena Rensen +14 more
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BioTechniques, 2015 Oligonucleotide microarrays allow the production of complex custom oligonucleotide libraries for nucleic acid detection–based applications such as fluorescence in situ hybridization (FISH). We have developed a PCR-free method to make single-stranded DNA (
Yusuf Murgha +6 more
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Current Issues in Molecular Biology, 2021 Northern blotting (NB), a gold standard for RNA detection, has lost its charm due to its hands-on nature, need for good quality RNA, and radioactivity.
Waqar Ahmad +3 more
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Organism-specific rRNA capture system for application in next-generation sequencing.
PLoS ONE, 2013 RNA-sequencing is a powerful tool in studying RNomics. However, the highly abundance of ribosomal RNAs (rRNA) and transfer RNA (tRNA) have predominated in the sequencing reads, thereby hindering the study of lowly expressed genes.
Sai-Kam Li +6 more
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Metabolic RNA labeling for probing RNA dynamics in bacteria [PDF]
Nucleic Acids Research, 2020 Abstract Metabolic labeling of RNAs with noncanonical nucleosides that are chemically active, followed by chemoselective conjugation with imaging probes or enrichment tags, has emerged as a powerful method for studying RNA transcription and degradation in eukaryotes.
Liying Meng +5 more
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Affinity Purification of RNA: Sequence-Specific Capture by Nonionic Morpholino Probes
BioTechniques, 1997 Nucleic acid isolation for amplification-based diagnostics requires techniques that do not co-purify inhibitors of DNA polymerases. Also, other requirements for an ideal sample preparation technology include ease of use, capability for automation, high ...
J.M. Wages +4 more
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Probing RNA Folding Pathways by RNA Fingerprinting [PDF]
Current Protocols in Nucleic Acid Chemistry, 2000 AbstractThis unit provides protocols for using native polyacrylamide gel electrophoresis to distinguish folding and unfolding conformers of RNA. It is useful for studying conformers that can exchange in a period of minutes or seconds, and that are thus difficult to study by solution‐based methods.
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Identification of artifactual microarray probe signals constantly present in multiple sample types
BioTechniques, 2012 The detection, identification, and quantitation of transcripts have evolved from simple Northern analysis, cDNA cloning, and sequencing to RT-PCR, microarrays, and now digital gene expression using ultra–high-throughput RNA sequencing (RNA-Seq).
Shihong Mao +3 more
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