Results 11 to 20 of about 1,363,084 (305)
, 2007 ‘Cultural probes’, since first being proposed and described by Bill Gaver and his colleagues, have been adapted and appropriated for a range of purposes within a variety of technology projects.
Frank Vetere +9 more
core +5 more sources
Novel endoribonucleases as central players in various pathways of eukaryotic RNA metabolism [PDF]
, 2010 For a long time it has been assumed that the decay of RNA in eukaryotes is mainly carried out by exoribonucleases, which is in contrast to bacteria, where endoribonucleases are well documented to initiate RNA degradation.
Tomecki, Rafał, Dziembowski, Andrzej
core +3 more sources
Gamma-FIT-PNAs as sensitive RNA probes. [PDF]
RSC Chem BiolA variety of oligonucleotide-based probes have been developed for specific and selective sensing of RNA and DNA. Among these, FIT-PNAs (forced intercalation-peptide nucleic acids) and FIT probes (DNA- and RNA-based sensors) have been studied for a ...
Gupta MK, Maree S, Yavin E.
europepmc +2 more sources
Sensitive visualization of SARS-CoV-2 RNA with CoronaFISH
Life Science Alliance, 2022 We design an RNA-FISH method to target the plus and minus strands of SARS-CoV-2 RNA, and apply it to image the virus in cell lines, lung tissue, and nasal swabs, by fluorescence and electron microscopy.
Elena Rensen +14 more
doaj +1 more source
BioTechniques, 2015 Oligonucleotide microarrays allow the production of complex custom oligonucleotide libraries for nucleic acid detection–based applications such as fluorescence in situ hybridization (FISH). We have developed a PCR-free method to make single-stranded DNA (
Yusuf Murgha +6 more
doaj +1 more source
Current Issues in Molecular Biology, 2021 Northern blotting (NB), a gold standard for RNA detection, has lost its charm due to its hands-on nature, need for good quality RNA, and radioactivity.
Waqar Ahmad +3 more
doaj +1 more source
Organism-specific rRNA capture system for application in next-generation sequencing.
PLoS ONE, 2013 RNA-sequencing is a powerful tool in studying RNomics. However, the highly abundance of ribosomal RNAs (rRNA) and transfer RNA (tRNA) have predominated in the sequencing reads, thereby hindering the study of lowly expressed genes.
Sai-Kam Li +6 more
doaj +1 more source
Metabolic RNA labeling for probing RNA dynamics in bacteria [PDF]
Nucleic Acids Research, 2020 Abstract Metabolic labeling of RNAs with noncanonical nucleosides that are chemically active, followed by chemoselective conjugation with imaging probes or enrichment tags, has emerged as a powerful method for studying RNA transcription and degradation in eukaryotes.
Liying Meng +5 more
openaire +2 more sources
Global Array-Based Transcriptomics from Minimal Input RNA Utilising an Optimal RNA Isolation Process Combined with SPIA cDNA Probes [PDF]
, 2011 Background: Technical advances in the collection of clinical material, such as laser capture microdissection and cell sorting, provide the advantage of yielding more refined and homogenous populations of cells.
Feuerstein, Giora +32 more
core +2 more sources
Maf1-mediated repression of RNA polymerase III transcription inhibits tRNA degradation via RTD pathway [PDF]
, 2012 tRNA precursors, which are transcribed by RNA polymerase III, undergo end-maturation, splicing and base modifications. Hypomodified tRNAs, such as tRNAVal(AAC), lacking 7-methylguanosine and 5-methylcytidine modifications, are subject to degradation by a
Boguta, Magdalena +3 more
core +1 more source