Results 11 to 20 of about 601,872 (302)
Sensitive visualization of SARS-CoV-2 RNA with CoronaFISH
Life Science Alliance, 2022 We design an RNA-FISH method to target the plus and minus strands of SARS-CoV-2 RNA, and apply it to image the virus in cell lines, lung tissue, and nasal swabs, by fluorescence and electron microscopy.
Elena Rensen +14 more
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Uncovering the expression patterns of chimeric transcripts using surveys of affymetrix GeneChips. [PDF]
, 2010 BACKGROUND: A chimeric transcript is a single RNA sequence which results from the transcription of two adjacent genes. Recent studies estimate that at least 4% of tandem human gene pairs may form chimeric transcripts. Affymetrix GeneChip data are used to
da Silva Camargo, R +4 more
core +2 more sources
BioTechniques, 2015 Oligonucleotide microarrays allow the production of complex custom oligonucleotide libraries for nucleic acid detection–based applications such as fluorescence in situ hybridization (FISH). We have developed a PCR-free method to make single-stranded DNA (
Yusuf Murgha +6 more
doaj +1 more source
Current Issues in Molecular Biology, 2021 Northern blotting (NB), a gold standard for RNA detection, has lost its charm due to its hands-on nature, need for good quality RNA, and radioactivity.
Waqar Ahmad +3 more
doaj +1 more source
Organism-specific rRNA capture system for application in next-generation sequencing.
PLoS ONE, 2013 RNA-sequencing is a powerful tool in studying RNomics. However, the highly abundance of ribosomal RNAs (rRNA) and transfer RNA (tRNA) have predominated in the sequencing reads, thereby hindering the study of lowly expressed genes.
Sai-Kam Li +6 more
doaj +1 more source
Tissue-specific Expression of Distinct Spectrin and Ankyrin Transcripts in Erythroid and Nonerythroid Cells [PDF]
, 1985 cDNA probes for three components of the erythroid membrane skeleton, α spectrin, β spectrin, and ankyrin, were obtained by using monospecific antibodies to screen a λgt11 expression vector library containing cDNA prepared from chicken erythroid poly(A)^+
Lazarides, Elias +3 more
core +3 more sources
Affinity Purification of RNA: Sequence-Specific Capture by Nonionic Morpholino Probes
BioTechniques, 1997 Nucleic acid isolation for amplification-based diagnostics requires techniques that do not co-purify inhibitors of DNA polymerases. Also, other requirements for an ideal sample preparation technology include ease of use, capability for automation, high ...
J.M. Wages +4 more
doaj +1 more source
Probing RNA Folding Pathways by RNA Fingerprinting [PDF]
Current Protocols in Nucleic Acid Chemistry, 2000 AbstractThis unit provides protocols for using native polyacrylamide gel electrophoresis to distinguish folding and unfolding conformers of RNA. It is useful for studying conformers that can exchange in a period of minutes or seconds, and that are thus difficult to study by solution‐based methods.
openaire +4 more sources
Identification of artifactual microarray probe signals constantly present in multiple sample types
BioTechniques, 2012 The detection, identification, and quantitation of transcripts have evolved from simple Northern analysis, cDNA cloning, and sequencing to RT-PCR, microarrays, and now digital gene expression using ultra–high-throughput RNA sequencing (RNA-Seq).
Shihong Mao +3 more
doaj +1 more source
Bio-Protocol, 2018 The protocol described here has been developed to detect RNA at the single cell level. Fluorescent probes hybridize to target RNAs and are detected by flow cytometry after multiple amplification steps. Different types of RNA can be detected such as mRNA,
Alice Coillard, Elodie Segura
doaj +1 more source