Results 21 to 30 of about 392,196 (263)
Bio-Protocol, 2018 The protocol described here has been developed to detect RNA at the single cell level. Fluorescent probes hybridize to target RNAs and are detected by flow cytometry after multiple amplification steps. Different types of RNA can be detected such as mRNA,
Alice Coillard, Elodie Segura
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Molecular Therapy: Nucleic Acids, 2022 We successfully fabricated threose nucleic acid (TNA)-based probes for real-time monitoring of target miRNA levels in cells. Our TNA probe is comprised of a fluorophore-labeled TNA reporter strand by partially hybridizing to a quencher-labeled TNA that ...
Fei Wang +5 more
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Probing the phenomics of noncoding RNA [PDF]
eLife, 2013 Genetic knockout experiments on mice confirm that some long noncoding RNA molecules have developmental functions.
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Miniaturized fluorescent RNA dot blot method for rapid quantitation of gene expression
BMC Biotechnology, 2004 Background RNA dot blot hybridization is a commonly used technique for gene expression assays. However, membrane based RNA dot/slot blot hybridization is time consuming, requires large amounts of RNA, and is less suited for parallel assays of more than ...
Yadetie Fekadu +4 more
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Using amino-labeled nucleotide probes for simultaneous single molecule RNA-DNA FISH. [PDF]
PLoS ONE, 2014 Using amino-labeled oligonucleotide probes, we established a simple, robust and low-noise method for simultaneous detection of RNA and DNA by fluorescence in situ hybridization, a highly useful tool to study the large pool of long non-coding RNAs being ...
Reelina Basu +5 more
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Probe Generation Directly from Small Numbers of Cells for DNA Microarray Studies
BioTechniques, 2003 Recently, we described a technique that allows us to prepare probes for expression profiling from 0.5–1 μg RNA without template or signal amplification.
C.C. Xiang +6 more
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Rapid, tunable, and multiplexed detection of RNA using convective array PCR
Communications Biology, 2023 Detection of RNA targets is typically achieved through RT-qPCR or RNAseq. RT-qPCR is rapid but limited in number and complexity of targets detected, while RNAseq is high-throughput but takes multiple days.
Andrew T. Sullivan +9 more
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Development of a Chemiluminescence-Based Ribonuclease Protection Assay
BioTechniques, 2002 The ribonuclease protection assay (RPA) is a widely used method for the detection and quantification of specific mRNA transcripts in a complex mixture of total RNA or mRNA molecules. While exhibiting many advantages over other RNA detection methods, RPAs
Christopher Rosenau +2 more
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Current Opinion in Structural Biology, 2019 RNA structure underpins many essential functions in biology. New chemical reagents and techniques for probing RNA structure in living cells have emerged in recent years. High-throughput, genome-wide techniques such as Structure-seq2 and DMS-MaPseq exploit nucleobase modification by dimethylsulfate (DMS) to obtain complete structuromes, and are ...
David, Mitchell +2 more
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Osmium tetroxide as a probe of RNA structure [PDF]
RNA, 2017 Structured RNAs have a central role in cellular function. The capability of structured RNAs to adopt fixed architectural structures or undergo dynamic conformational changes contributes to their diverse role in the regulation of gene expression. Although numerous biophysical and biochemical tools have been developed to study structured RNAs, there is a
Zhang, Jing +4 more
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