Results 21 to 30 of about 601,872 (302)
Molecular Therapy: Nucleic Acids, 2022 We successfully fabricated threose nucleic acid (TNA)-based probes for real-time monitoring of target miRNA levels in cells. Our TNA probe is comprised of a fluorophore-labeled TNA reporter strand by partially hybridizing to a quencher-labeled TNA that ...
Fei Wang +5 more
doaj +1 more source
Electrochemical detection of the toxic dinoflagellate Alexandrium ostenfeldii with a DNA-biosensor [PDF]
, 2005 The steady rise of observations of harmful or toxic algal blooms throughout the world in the past decades constitute a menace for coastal ecosystems and human interests.
Huljic, S. +3 more
core +1 more source
Using amino-labeled nucleotide probes for simultaneous single molecule RNA-DNA FISH. [PDF]
PLoS ONE, 2014 Using amino-labeled oligonucleotide probes, we established a simple, robust and low-noise method for simultaneous detection of RNA and DNA by fluorescence in situ hybridization, a highly useful tool to study the large pool of long non-coding RNAs being ...
Reelina Basu +5 more
doaj +1 more source
Noncoder : a web interface for exon array-based detection of long non-coding RNAs [PDF]
, 2012 Due to recent technical developments, a high number of long non-coding RNAs (lncRNAs) have been discovered in mammals. Although it has been shown that lncRNAs are regulated differently among tissues and disease statuses, functions of these transcripts ...
Braun, Thomas +3 more
core +1 more source
Miniaturized fluorescent RNA dot blot method for rapid quantitation of gene expression
BMC Biotechnology, 2004 Background RNA dot blot hybridization is a commonly used technique for gene expression assays. However, membrane based RNA dot/slot blot hybridization is time consuming, requires large amounts of RNA, and is less suited for parallel assays of more than ...
Yadetie Fekadu +4 more
doaj +1 more source
SnapShot: RNA Structure Probing Technologies
Cell, 2018 Chemical probing coupled to high-throughput sequencing offers a flexible approach to uncover many aspects of RNA structure relevant to its cellular function. With a wide variety of chemical probes available that each report on different features of RNA molecules, a broad toolkit exists for investigating in vivo and in vitro RNA structure and ...
Paul D, Carlson +4 more
openaire +2 more sources
Current Opinion in Structural Biology, 2019 RNA structure underpins many essential functions in biology. New chemical reagents and techniques for probing RNA structure in living cells have emerged in recent years. High-throughput, genome-wide techniques such as Structure-seq2 and DMS-MaPseq exploit nucleobase modification by dimethylsulfate (DMS) to obtain complete structuromes, and are ...
David, Mitchell +2 more
openaire +3 more sources
Probe Generation Directly from Small Numbers of Cells for DNA Microarray Studies
BioTechniques, 2003 Recently, we described a technique that allows us to prepare probes for expression profiling from 0.5–1 μg RNA without template or signal amplification.
C.C. Xiang +6 more
doaj +1 more source
Rapid, tunable, and multiplexed detection of RNA using convective array PCR
Communications Biology, 2023 Detection of RNA targets is typically achieved through RT-qPCR or RNAseq. RT-qPCR is rapid but limited in number and complexity of targets detected, while RNAseq is high-throughput but takes multiple days.
Andrew T. Sullivan +9 more
doaj +1 more source
Physico-chemical foundations underpinning microarray and next-generation sequencing experiments [PDF]
, 2013 Hybridization of nucleic acids on solid surfaces is a key process involved in high-throughput technologies such as microarrays and, in some cases, next-generation sequencing (NGS).
A. Buhot +70 more
core +3 more sources