Affinity Purification of RNA: Sequence-Specific Capture by Nonionic Morpholino Probes
BioTechniques, 1997 Nucleic acid isolation for amplification-based diagnostics requires techniques that do not co-purify inhibitors of DNA polymerases. Also, other requirements for an ideal sample preparation technology include ease of use, capability for automation, high ...
J.M. Wages +4 more
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Probing RNA Folding Pathways by RNA Fingerprinting [PDF]
Current Protocols in Nucleic Acid Chemistry, 2000 AbstractThis unit provides protocols for using native polyacrylamide gel electrophoresis to distinguish folding and unfolding conformers of RNA. It is useful for studying conformers that can exchange in a period of minutes or seconds, and that are thus difficult to study by solution‐based methods.
openaire +4 more sources
RNA-specific ribonucleotidyl transferases [PDF]
, 2007 RNA-specific nucleotidyl transferases (rNTrs) are a diverse family of template-independent polymerases that add ribonucleotides to the 3'-ends of RNA molecules.
Keller, W. +3 more
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Identification of artifactual microarray probe signals constantly present in multiple sample types
BioTechniques, 2012 The detection, identification, and quantitation of transcripts have evolved from simple Northern analysis, cDNA cloning, and sequencing to RT-PCR, microarrays, and now digital gene expression using ultra–high-throughput RNA sequencing (RNA-Seq).
Shihong Mao +3 more
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An essential role of KREPB4 in RNA editing and structural integrity of the editosome in Trypanosoma brucei [PDF]
, 2007 RNA editing in the sleeping sickness parasite Trypanosoma brucei remodels mitochondrial transcripts by the addition and deletion of uridylates as specified by guide RNAs.
Schnaufer, Achim; id_orcid +4 more
core +1 more source
An improved method for surface immobilisation of RNA: application to small Non-Coding RNA - mRNA pairing [PDF]
, 2013 Characterisation of RNA and its intermolecular interactions is increasing in importance as the inventory of known RNA functions continues to expand. RNA-RNA interactions are central to post-transcriptional gene regulation mechanisms in bacteria, and the ...
Darren M. Gowers (485354) +29 more
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Bio-Protocol, 2018 The protocol described here has been developed to detect RNA at the single cell level. Fluorescent probes hybridize to target RNAs and are detected by flow cytometry after multiple amplification steps. Different types of RNA can be detected such as mRNA,
Alice Coillard, Elodie Segura
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Crystal structure of Schmallenberg orthobunyavirus nucleoprotein-RNA complex reveals a novel RNA sequestration mechanism [PDF]
, 2013 Schmallenberg virus (SBV) is a newly emerged orthobunyavirus (family Bunyaviridae) that has caused severe disease in the offspring of farm animals across Europe.
Richard M. Elliott +16 more
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Axon micro-dissection and transcriptome profiling reveals the in vivo RNA content of fully differentiated myelinated motor axons [PDF]
, 2020 Axonal protein synthesis has been shown to play a role in developmental and regenerative growth, as well as in the maintenance of the axoplasm in steady state.
Sotelo-Silveira, José Roberto +3 more
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Molecular Therapy: Nucleic Acids, 2022 We successfully fabricated threose nucleic acid (TNA)-based probes for real-time monitoring of target miRNA levels in cells. Our TNA probe is comprised of a fluorophore-labeled TNA reporter strand by partially hybridizing to a quencher-labeled TNA that ...
Fei Wang +5 more
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