Results 41 to 50 of about 1,363,084 (305)

Comparison of different bead-beating RNA extraction strategies: An optimized method for filamentous fungi [PDF]

, 2012
Molecular studies, especially in relation to the activity of secondary metabolite gene clusters, require the ability to extract good quality RNA from fungal biomass.
Magan, Naresh, Leite, Goncalo M., Ángel Medina, Naresh Magan, Gonçalo M. Leite, Medina, Angel   +5 more
core   +1 more source

Detection of genome-scale ordered RNA structure (GORS) in genomes of positive-stranded RNA viruses:Implications for virus evolution and host persistence [PDF]

, 2004
Discrete RNA secondary and higher-order structures, typically local in extent, play a fundamental role in RNA virus replication. Using new bioinformatics analysis methods, we have identified genome-scale ordered RNA structure (GORS) in many genera and ...
Evans, David J, Simmonds, Peter, Tuplin, Andrew, Tuplin, A, Evans, DJ, Simmonds, P   +5 more
core   +1 more source

An improved in vivo tethering assay with single molecule FISH reveals that a nematode Nanos enhances reporter expression and mRNA stability

, 2021
Robust methods are critical for testing the in vivo regulatory mechanism of RNA binding proteins. Here we report improvement of a protein–mRNA tethering assay to probe the function of an RNA binding protein in its natural context within the C.
Kimble, Judith, Lynch, Tina R., Aoki, Scott T., Doenier, Jonathan   +3 more
core   +1 more source

Differential polyadenylation of ribosomal RNA during post-transcriptional processing in Leishmania. [PDF]

, 2005
The protozoan parasite Leishmania belongs to the most ancient eukaryotic lineages and this is reflected in several distinctive biological features, such as eukaryotic polycistronic transcription and RNA trans-splicing.
Laurent, T., Chappuis, F., Llanos-Cuentas, A., Llanos-Cuentas, A, V. YARDLEY, F. CHAPPUIS, Laurent, T, T. LAURENT, Dujardin, J. C., Vandesompele, J, J.-C. DUJARDIN, DE DONCKER, S, S. RIJAL, Decuypere, S., S. DECUYPERE, Yardley, V, De Donckeri, S, Vandesompele, Jo, Rijal, S., J. AREVALO, Vandesompele, J., S. DE DONCKER, J. VANDESOMPELE, Yardley, V., Chappuis, F, Arevalo, J., Arevalo, J, Rijal, S, De Doncker, S., A. LLANOS-CUENTAS, Dujardin, JC, Decuypere, S   +31 more
core   +1 more source

Discovery of RNA-binding proteins and characterization of their dynamic responses by enhanced RNA interactome capture

Nature Communications, 2018
RNA interactome capture allows the detailed investigation of RNA-bound proteomes. Here the authors describe enhanced RNA-interactome capture using LNA-modified probes for increased sensitivity and specificity.
Joel I. Perez-Perri, Birgit Rogell, Thomas Schwarzl, Frank Stein, Yang Zhou, Mandy Rettel, Annika Brosig, Matthias W. Hentze   +7 more
doaj   +1 more source

Nanoribbon-Based Electronic Detection of a Glioma-Associated Circular miRNA

Biosensors, 2021
Nanoribbon chips, based on “silicon-on-insulator” structures (SOI-NR chips), have been fabricated. These SOI-NR chips, whose surface was sensitized with covalently immobilized oligonucleotide molecular probes (oDNA probes), have been employed for the ...
Yuri D. Ivanov, Kristina A. Malsagova, Vladimir P. Popov, Tatyana O. Pleshakova, Andrey F. Kozlov, Rafael A. Galiullin, Ivan D. Shumov, Svetlana I. Kapustina, Fedor V. Tikhonenko, Vadim S. Ziborov, Alexander Yu. Dolgoborodov, Oleg F. Petrov, Olga A. Gadzhieva, Boris A. Bashiryan, Vadim N. Shimansky, Natalia V. Potoldykova, Dmitry V. Enikeev, Dmitry Yu. Usachev, Alexander I. Archakov   +18 more
doaj   +1 more source

Ovarian Sex Cord Stromal Tumors in Children and Adolescents—The European Standard Clinical Practice Recommendations

Pediatric Blood &Cancer, EarlyView.
ABSTRACT As part of the European Cooperative Study Group for Paediatric Rare Tumours initiative, we developed standard clinical practice guidelines for ovarian sex cord stromal tumors, based on comprehensive national and international cohort analyses, literature review, and a final expert consensus conference.
Dominik T. Schneider, Kris‐Ann Schultz, Nora Schnitzler, Michael Abele, Tal Ben‐Ami, Ewa Bien, Gianni Bisogno, Ines B. Brecht, Maja Cesen, Andrea Ferrari, Michaela Kuhlen, Malgorzata A. Krawczyk, Ricardo Lopez‐Almaraz, Yves Reguerre, Calogero Virgone, Daniel Orbach   +15 more
wiley   +1 more source

Domain orientation in the RNA helicase YxiN and the role of conformational changes for RNA unwinding [PDF]

, 2010
The RNA helicase YxiN from Bacillus subtilis is a member of the family of DEAD box proteins. YxiN is able to unwind RNA double strands in an ATP-dependent manner. The ability to catalyse RNA rearrangement is in vivo presumably necessary for the bacterial
Karow, Anne R.
core   +1 more source

Infant Embryonal CNS Tumors: Molecular Insights and Treatment Considerations for Contemporary Pediatric Neuro‐Oncology

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Background Embryonal tumors comprise the majority of malignant central nervous system (CNS) neoplasms diagnosed in children under 3 years of age. Compared with their counterparts in older children, these tumors exhibit distinct molecular biology and a more aggressive clinical phenotype, while their management is complicated by the heightened ...
Sudarshawn Damodharan, Mariah Wright‐Nadkarni, Mohamed S. Abdelbaki, Girish Dhall   +3 more
wiley   +1 more source

Detection of NASBA amplified bacterial tmRNA molecules on SLICSel designed microarray probes

BMC Biotechnology, 2011
Background We present a comprehensive technological solution for bacterial diagnostics using tmRNA as a marker molecule. A robust probe design algorithm for microbial detection microarray is implemented.
Toome Kadri, Parkel Sven, Palta Priit, Glynn Barry, Kaplinski Lauris, Scheler Ott, Maher Majella, Barry Thomas, Remm Maido, Kurg Ants   +9 more
doaj   +1 more source