Results 61 to 70 of about 1,363,084 (305)

Fast-exchanging spirocyclic rhodamine probes for aptamer-based super-resolution RNA imaging

Nature Communications, 2023
Live-cell RNA imaging with high spatial and temporal resolution remains a major challenge. Here we report the development of RhoBAST:SpyRho, a fluorescent light-up aptamer (FLAP) system ideally suited for visualizing RNAs in live or fixed cells with ...
Daniel Englert, Eva-Maria Burger, Franziska Grün, Mrigank S. Verma, Jens Lackner, Marko Lampe, Bastian Bühler, Janin Schokolowski, G. Ulrich Nienhaus, Andres Jäschke, Murat Sunbul   +10 more
doaj   +1 more source

Trim25 is an RNA-specific activator of Lin28a/TuT4-mediated uridylation [PDF]

, 2014
RNA binding proteins have thousands of cellular RNA targets and often exhibit opposite or passive molecular functions. Lin28a is a conserved RNA binding protein involved in pluripotency and tumorigenesis that was previously shown to trigger TuT4-mediated
Nowak, Jakub S., Roy Choudhury, Nila, Rappsilber, Juri, Spoel, Steven H, Nila Roy Choudhury, Nowak, Jakub S, Zuo, Juan, Michlewski, Gracjan, Juan Zuo, Juri Rappsilber, Choudhury, Nila Roy, Steven H. Spoel, Gracjan Michlewski, Jakub S. Nowak, Spoel, Steven H.   +14 more
core   +1 more source

Phosphatidylinositol 4‐kinase as a target of pathogens—friend or foe?

FEBS Letters, EarlyView.
This graphical summary illustrates the roles of phosphatidylinositol 4‐kinases (PI4Ks). PI4Ks regulate key cellular processes and can be hijacked by pathogens, such as viruses, bacteria and parasites, to support their intracellular replication. Their dual role as essential host enzymes and pathogen cofactors makes them promising drug targets.
Ana C. Mendes, Guilherme M. Azevedo, Amanda P. Barcellos, Diana Bahia   +3 more
wiley   +1 more source

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

A cross strain Plasmodium falciparum microarray optimized for the transcriptome analysis of Plasmodium falciparum patient derived isolates

Genomics Data, 2016
Malarial parasite P. falciparum, an apicomplexan protozoan has a 23.3 MB nuclear genome and encodes ~5600 transcripts. The genetic diversity of the parasite within and across geographical zones is a challenge to gene expression studies which are ...
Amit Kumar Subudhi, P.A. Boopathi, Sheetal Middha, Jyoti Acharya, Sudha Narayana Rao, Raja C. Mugasimangalam, Paramendra Sirohi, Sanjay K. Kochar, Dhanpat K. Kochar, Ashis Das   +9 more
doaj   +1 more source

Insights into the development of chemical probes for RNA [PDF]

Nucleic Acids Research, 2018
Over the past decade, the RNA revolution has revealed thousands of non-coding RNAs that are essential for cellular regulation and are misregulated in disease. While the development of methods and tools to study these RNAs has been challenging, the power and promise of small molecule chemical probes is increasingly recognized.
Brittany S Morgan, Jordan E Forte, Amanda E Hargrove   +2 more
openaire   +2 more sources

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Diversity and complexity in neural organoids

FEBS Letters, EarlyView.
Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley   +1 more source

A molecular beacon-based approach for live-cell imaging of RNA transcripts with minimal target engineering at the single-molecule level

Scientific Reports, 2017
Analysis of RNA dynamics and localization at the single-molecule level in living cells has been predominantly achieved by engineering target RNAs with large insertions of tandem repeat sequences that are bound by protein-based or oligonucleotide-based ...
Mingming Chen, Zhao Ma, Xiaotian Wu, Shiqi Mao, Yantao Yang, Jie Tan, Christopher J. Krueger, Antony K. Chen   +7 more
doaj   +1 more source

Osmium tetroxide as a probe of RNA structure [PDF]

RNA, 2017
Structured RNAs have a central role in cellular function. The capability of structured RNAs to adopt fixed architectural structures or undergo dynamic conformational changes contributes to their diverse role in the regulation of gene expression. Although numerous biophysical and biochemical tools have been developed to study structured RNAs, there is a
Zhang, Jing, Li, Danbin, Zhang, Jun, Chen, Dongrong, Murchie, Alastair I.H.   +4 more
openaire   +2 more sources