Results 71 to 80 of about 1,363,084 (305)

Identification of novel components of Trypanosoma brucei editosomes [PDF]

, 2003
The editosome is a multiprotein complex that catalyzes the insertion and deletion of uridylates that occurs during RNA editing in trypanosomatids. We report the identification of nine novel editosome proteins in Trypanosoma brucei.
Schnaufer, Achim; id_orcid, Wang, Bingbing, Salavati, Reza, Stuart, Kenneth, Carmean, Nicole, Panigrahi, Aswini K, Ernst, Nancy L   +6 more
core   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

HybriSeq: probe-based device-free single-cell RNA profiling

Communications Biology
We have developed the HybriSeq method for single-cell RNA profiling, which utilizes in situ hybridization of multiple probes for targeted transcripts, followed by split-pool barcoding and sequencing analysis of the probes. We have shown that HybriSeq can
Daniel Foyt, David Brown, Shuqin Zhou, Brittany Moser, Qin Zhu, Zev J. Gartner, Bo Huang   +6 more
doaj   +1 more source

In situ detection of non-polyadenylated RNA molecules using Turtle Probes and target primed rolling circle PRINS

BMC Biotechnology, 2007
Background In situ detection is traditionally performed with long labeled probes often followed by a signal amplification step to enhance the labeling. Whilst short probes have several advantages over long probes (e.g.
Hamilton-Dutoit Stephen, Zajac Magdalena, Lohmann Jakob S, Stougaard Magnus, Koch Jørn   +4 more
doaj   +1 more source

Prediction of RNA Secondary Structure Including Kissing Hairpin Motifs [PDF]

, 2010
Theis C, Janssen S, Giegerich R. Prediction of RNA Secondary Structure Including Kissing Hairpin Motifs. In: Moulton V, Singh M, eds. Algorithms in Bioinformatics. 10th international workshop (WABI 2010), proceedings. Lecture Notes in Bioinformatics. Vol
Corinna Theis, Theis, Corinna, Moulton, Vincent, Singh, Mona, Stefan Janssen, Janssen, Stefan ; https://orcid.org/, Robert Giegerich, Giegerich, Robert   +7 more
core   +1 more source

The mutual interaction of glycolytic enzymes and RNA in post-transcriptional regulation

, 2022
Wegener M, Dietz K-J. The mutual interaction of glycolytic enzymes and RNA in post-transcriptional regulation. RNA . 2022.About three decades ago, researchers suggested that metabolic enzymes participate in cellular processes that are unrelated to their ...
Dietz, Karl-Josef, Karl-Josef Dietz, Wegener, Melanie, Melanie Wegener   +3 more
core   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source

RNA Isolation and Northern Blot Analysis

Bio-Protocol, 2014
The northern blot is a technique used in molecular biology research to study gene expression by detection of RNA in a sample. With northern blotting it is possible to observe particular gene expression levels during differentiation, morphogenesis, as ...
Ying Liao, To Fung, Mei Huang, Shouguo Fang, Yanxin Zhong, Dingxiang Liu   +5 more
doaj   +1 more source

SNP detection in mRNA in living cells using allele specific FRET probes. [PDF]

PLoS ONE, 2013
Live mRNA detection allows real time monitoring of specific transcripts and genetic alterations. The main challenge of live genetic detection is overcoming the high background generated by unbound probes and reaching high level of specificity with ...
Liya Dahan, Lingyan Huang, Ranit Kedmi, Mark A Behlke, Dan Peer   +4 more
doaj   +1 more source

Organizing the interface—Plasma membrane architecture and receptor dynamics in virus‐cell interactions

FEBS Letters, EarlyView.
Plasma membranes contain dynamic nanoscale domains that organize lipids and receptors. Because viruses operate at similar scales, this architecture shapes early infection steps, including attachment, receptor engagement, and entry. Using influenza A virus and HIV‐1 as examples, we highlight how receptor nanoclusters, multivalent glycan interactions ...
Jan Schlegel, Christian Sieben
wiley   +1 more source