Results 71 to 80 of about 601,872 (302)
From monoclonal antibody to gene for a neuron-specific glycoprotein in Drosophila [PDF]
, 1985 A monoclonal antibody (MAb24B10), derived from mice immunized with Drosophila retina, exclusively stains photoreceptor cells in the retina and their axonal projections to the optic ganglia. The antigen (Ag24B10) is a 160-kDa glycoprotein comprising about
Benzer, Seymour +2 more
core
Transcriptional regulation of prostate kallikrein-like genes by androgen. [PDF]
, 1992 Using gene-specific synthetic oligonucleotides the expression and regulation of kallikrein-like genes in the human prostatic cancer cell line LNCaP were studied.
Fittler, Friedrich +2 more
core +1 more source
FEBS Letters, EarlyView.In this study, we found that human cervical‐derived adipocytes maintain intracellular iron level by regulating the expression of iron transport‐related proteins during adrenergic stimulation. Melanotransferrin is predicted to interact with transferrin receptor 1 based on in silico analysis.
Rahaf Alrifai +9 more
wiley +1 more source
RNA Isolation and Northern Blot Analysis
Bio-Protocol, 2014 The northern blot is a technique used in molecular biology research to study gene expression by detection of RNA in a sample. With northern blotting it is possible to observe particular gene expression levels during differentiation, morphogenesis, as ...
Ying Liao +5 more
doaj +1 more source
Development of peptide nucleic acid probes for detection of the HER2 oncogene. [PDF]
PLoS ONE, 2013 Peptide nucleic acids (PNAs) have gained much interest as molecular recognition tools in biology, medicine and chemistry. This is due to high hybridization efficiency to complimentary oligonucleotides and stability of the duplexes with RNA or DNA.
Belhu Metaferia +13 more
doaj +1 more source
13C-direct detected NMR experiments for the sequential J-based resonance assignment of RNA oligonucleotides [PDF]
, 2010 We present here a set of 13C-direct detected NMR experiments to facilitate the resonance assignment of RNA oligonucleotides. Three experiments have been developed: (1) the (H)CC-TOCSY-experiment utilizing a virtual decoupling scheme to assign the ...
Christian Richter +61 more
core +1 more source
Probing RNA Structure by Lead Cleavage [PDF]
Current Protocols in Nucleic Acid Chemistry, 2000 AbstractLead cleavage causes a transesterification reaction that breaks the 5′,3′‐phosphodiester backbone of RNA, leaving a 2′,3′‐cyclic phosphate and a 5′‐hydroxyl. Since the efficiency of the reaction at the 2′‐hydroxyl is related to steric and chemical constraints on particular 2′‐hydroxyls embedded in the RNA, this reaction can be used to examine ...
openaire +3 more sources
Tau acetylation at K331 has limited impact on tau pathology in vivo
FEBS Letters, EarlyView.We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto +3 more
wiley +1 more source
HybriSeq: probe-based device-free single-cell RNA profiling
Communications BiologyWe have developed the HybriSeq method for single-cell RNA profiling, which utilizes in situ hybridization of multiple probes for targeted transcripts, followed by split-pool barcoding and sequencing analysis of the probes. We have shown that HybriSeq can
Daniel Foyt +6 more
doaj +1 more source
BMC Biotechnology, 2007 Background In situ detection is traditionally performed with long labeled probes often followed by a signal amplification step to enhance the labeling. Whilst short probes have several advantages over long probes (e.g.
Hamilton-Dutoit Stephen +4 more
doaj +1 more source