Results 81 to 90 of about 1,363,084 (305)

Enriching Bacteria-Specific RNA From Host Samples Before NGS With Transcript-Capture

Bio-Protocol
Pathogen gene expression from host samples is often challenging to study due to low signal and high host RNA background. PCR probes have been recently used to hybridize and extract bacterial sequences from next-generation sequencing (NGS) libraries ...
Eleanor Lamont, Richard Jones, Jessica Assadi, Shuyi Ma, David Sherman   +4 more
doaj   +1 more source

Development of peptide nucleic acid probes for detection of the HER2 oncogene. [PDF]

PLoS ONE, 2013
Peptide nucleic acids (PNAs) have gained much interest as molecular recognition tools in biology, medicine and chemistry. This is due to high hybridization efficiency to complimentary oligonucleotides and stability of the duplexes with RNA or DNA.
Belhu Metaferia, Jun S Wei, Young K Song, Jennifer Evangelista, Konrad Aschenbach, Peter Johansson, Xinyu Wen, Qingrong Chen, Albert Lee, Heidi Hempel, Jinesh S Gheeya, Stephanie Getty, Romel Gomez, Javed Khan   +13 more
doaj   +1 more source

Diverging affinity of tospovirus RNA silencing suppressor proteins, NSs, for various RNA duplex molecules [PDF]

, 2010
The tospovirus NSs protein was previously shown to suppress the antiviral RNA silencing mechanism in plants. Here the biochemical analysis of NSs proteins from different tospoviruses, using purified NSs or NSs containing cell extracts, is described.
Kormelink, R.J.M., Rik Huismann, Esther Schnettler, Rob Goldbach, Goldbach, R., Marcel Prins, Goldbach, R.W., Kormelink, R., Hemmes, H., Prins, M., Huismann, R., Hans Hemmes, Huisman, R., Hemmes, J.C., Schnettler, E., Richard Kormelink, Prins, M.W.   +16 more
core   +1 more source

Septin 9 PB domains coordinate centrosome positioning and microtubule acetylation to control epithelial polarity

FEBS Letters, EarlyView.
Septin 9 polybasic domains couple phosphoinositide‐rich membrane binding to centrosome positioning, Golgi organization, and microtubule acetylation to control epithelial polarity. Their loss disrupts this axis, causing centrosome mispositioning, Golgi fragmentation, reduced microtubule acetylation, and polarity inversion via upregulation of the ...
Ting ting Cai, Mohyeddine Omrane, Nassima Benzoubir, Didier Samuel, Ama Gassama‐Diagne   +4 more
wiley   +1 more source

A gene-specific RNA enrichment protocol for nanopore direct-RNA sequencing.

PLoS ONE
Oxford Nanopore direct-RNA sequencing, a third-generation sequencing technology, allows for the analysis of native RNA molecules in their natural cellular state.
Maja Bele Dyrendalsli, Cecilie Løkke, Christer Einvik   +2 more
doaj   +1 more source

Fluorescent Probes for Nucleic Acid Visualization in Fixed and Live Cells

Molecules, 2013
This review analyses the literature concerning non-fluorescent and fluorescent probes for nucleic acid imaging in fixed and living cells from the point of view of their suitability for imaging intracellular native RNA and DNA. Attention is mainly paid to
Alexandre S. Boutorine, Darya S. Novopashina, Olga A. Krasheninina, Karine Nozeret, Alya G. Venyaminova   +4 more
doaj   +1 more source

Construction and screening of a pea root cDNA library [PDF]

, 1988
A cDNA library was synthesized using poly(A)(^+) RNA purified from the total RNA from roots of pea (Pisum sativum L.). Ten representative clones encoding abundant root proteins were isolated from the library after screening using colony hybridization ...
Choy, Andy Kwan Yan
core  

Comparison of quantitative real time PCR with Sequencing and ribosomal RNA-FISH for the identification of fungi in Formalin fixed, paraffin-embedded tissue specimens [PDF]

, 2011
Background: Identification of the causative agents of invasive fungal infections (IFI) is critical for guiding antifungal therapy. Cultures remain negative in a substantial number of IFI cases.
Rickerts Volker, Ko Daisy L, Fredricks, David N., Prasanna D Khot, David N Fredricks, David Myerson, Khot Prasanna D, Myerson David, Ko, Daisy L., Khot, Prasanna D., Myerson, David, Volker Rickerts, Lambrecht, Evelyn, Lambrecht Evelyn, Rickerts, Volker, Fredricks David N, Daisy L Ko, Evelyn Lambrecht   +17 more
core   +2 more sources

Rab14 regulates the transport of human papillomavirus to the trans‐Golgi network for infectious cell entry

FEBS Letters, EarlyView.
This study reveals that the small GTPase Rab14 is necessary for human papillomavirus (HPV) infection and plays an essential role in the transport of virions to the trans‐Golgi network (TGN). HPV in the early endosome (EE), which harbors GTP‐bound Rab14, is transported to the TGN through the switch of Rab14 from its GTP‐bound to GDP‐bound form.
Yoshiyuki Ishii, Iwao Kukimoto
wiley   +1 more source

Comparison of Different In Situ Hybridization Techniques for the Detection of Various RNA and DNA Viruses

Viruses, 2018
In situ hybridization (ISH) is a technique to determine potential correlations between viruses and lesions. The aim of the study was to compare ISH techniques for the detection of various viruses in different tissues.
Vanessa M. Pfankuche, Kerstin Hahn, Rogier Bodewes, Florian Hansmann, André Habierski, Ann-Kathrin Haverkamp, Stephanie Pfaender, Stephanie Walter, Christine Baechlein, Alexander Postel, Eike Steinmann, Paul Becher, Albert Osterhaus, Wolfgang Baumgärtner, Christina Puff   +14 more
doaj   +1 more source