Error, reproducibility and sensitivity : a pipeline for data processing of Agilent oligonucleotide expression arrays [PDF]
, 2010 Background Expression microarrays are increasingly used to obtain large scale transcriptomic information on a wide range of biological samples. Nevertheless, there is still much debate on the best ways to process data, to design experiments and analyse ...
Bowen, Helen C. +6 more
core +2 more sources
Metabolic RNA labeling for probing RNA dynamics in bacteria [PDF]
Nucleic Acids Research, 2020 Abstract Metabolic labeling of RNAs with noncanonical nucleosides that are chemically active, followed by chemoselective conjugation with imaging probes or enrichment tags, has emerged as a powerful method for studying RNA transcription and degradation in eukaryotes.
Liying Meng +5 more
openaire +2 more sources
Development of a Chemiluminescence-Based Ribonuclease Protection Assay
BioTechniques, 2002 The ribonuclease protection assay (RPA) is a widely used method for the detection and quantification of specific mRNA transcripts in a complex mixture of total RNA or mRNA molecules. While exhibiting many advantages over other RNA detection methods, RPAs
Christopher Rosenau +2 more
doaj +1 more source
Transcription of the sporulation gene ssgA is activated by the IcIR-type regulator SsgR in a whi-independent manner in Streptomyces coelicolor A3(2). [PDF]
, 2004 SsgA plays an important role in the control of sporulation-specific cell division and morphogenesis of streptomycetes, and ssgA null mutants have a rare conditionally non-sporulating phenotype.
Bentley +42 more
core +2 more sources
Nanoribbon-Based Electronic Detection of a Glioma-Associated Circular miRNA
Biosensors, 2021 Nanoribbon chips, based on “silicon-on-insulator” structures (SOI-NR chips), have been fabricated. These SOI-NR chips, whose surface was sensitized with covalently immobilized oligonucleotide molecular probes (oDNA probes), have been employed for the ...
Yuri D. Ivanov +18 more
doaj +1 more source
Nature Communications, 2018 RNA interactome capture allows the detailed investigation of RNA-bound proteomes. Here the authors describe enhanced RNA-interactome capture using LNA-modified probes for increased sensitivity and specificity.
Joel I. Perez-Perri +7 more
doaj +1 more source
Northern Blot with IR Fluorescent Probes: Strategies for Probe Preparation
Bio-Protocol, 2019 Northern blot is a molecular biology technique that can detect, quantify, and determine the molecular weight of RNA. Recently, we published a protocol utilizing near-infrared (IR) fluorescent probes in Northern blot (irNorthern).
Christopher Fields +4 more
doaj +1 more source
FEBS Letters, EarlyView.Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi +5 more
wiley +1 more source
The T7-primer is a source of experimental bias and introduces variability between microarray platforms. [PDF]
PLoS ONE, 2008 Eberwine(-like) amplification of mRNA adds distinct 6-10 bp nucleotide stretches to the 5' end of amplified RNA transcripts. Analysis of over six thousand microarrays reveals that probes containing motifs complementary to these stretches are associated ...
Ron M Kerkhoven +6 more
doaj +1 more source
Identification of a functional genetic variant driving racially dimorphic platelet gene expression of the thrombin receptor regulator, PCTP. [PDF]
, 2017 Platelet activation in response to stimulation of the Protease Activated Receptor 4 (PAR4) receptor differs by race. One factor that contributes to this difference is the expression level of Phosphatidylcholine Transfer Protein (PCTP), a regulator of ...
Bray, Paul F. +5 more
core +3 more sources