Results 31 to 40 of about 1,363,084 (305)

Probing RNA structure in vivo

Current Opinion in Structural Biology, 2019
RNA structure underpins many essential functions in biology. New chemical reagents and techniques for probing RNA structure in living cells have emerged in recent years. High-throughput, genome-wide techniques such as Structure-seq2 and DMS-MaPseq exploit nucleobase modification by dimethylsulfate (DMS) to obtain complete structuromes, and are ...
David, Mitchell, Sarah M, Assmann, Philip C, Bevilacqua   +2 more
openaire   +3 more sources

Miniaturized fluorescent RNA dot blot method for rapid quantitation of gene expression

BMC Biotechnology, 2004
Background RNA dot blot hybridization is a commonly used technique for gene expression assays. However, membrane based RNA dot/slot blot hybridization is time consuming, requires large amounts of RNA, and is less suited for parallel assays of more than ...
Yadetie Fekadu, Sandvik Arne K, Bergum Hallgeir, Norsett Kristin, Laegreid Astrid   +4 more
doaj   +1 more source

Using amino-labeled nucleotide probes for simultaneous single molecule RNA-DNA FISH. [PDF]

PLoS ONE, 2014
Using amino-labeled oligonucleotide probes, we established a simple, robust and low-noise method for simultaneous detection of RNA and DNA by fluorescence in situ hybridization, a highly useful tool to study the large pool of long non-coding RNAs being ...
Reelina Basu, Lan-Tian Lai, Zhenyu Meng, Jun Wu, Fangwei Shao, Li-Feng Zhang   +5 more
doaj   +1 more source

Probing the phenomics of noncoding RNA [PDF]

eLife, 2013
Genetic knockout experiments on mice confirm that some long noncoding RNA molecules have developmental functions.
openaire   +3 more sources

Formamide significantly enhances the efficiency of chemical adenylation of RNA sequencing ligation adaptors [PDF]

, 2023
Preadenylated single-stranded DNA ligation adaptors are essential reagents in many next generation RNA sequencing library preparation protocols. These oligonucleotides can be adenylated enzymatically or chemically.
Watts, Jonathan K, Hildebrand, Samuel R, Hedger, Adam K, Khvorova, Anastasia, Yamada, Ken   +4 more
core   +1 more source

Complete probabilistic analysis of RNA shapes [PDF]

, 2006
Voß B, Giegerich R, Rehmsmeier M. Complete probabilistic analysis of RNA shapes. BMC Biology. 2006;4(1): 5.Background: Soon after the first algorithms for RNA folding became available, it was recognised that the prediction of only one energetically ...
Marc Rehmsmeier, Voß, Björn, Rehmsmeier, Marc, Björn Voß, Rehmsmeier Marc, Robert Giegerich, Giegerich, Robert, Giegerich Robert, Voß Björn   +8 more
core   +1 more source

Rapid, tunable, and multiplexed detection of RNA using convective array PCR

Communications Biology, 2023
Detection of RNA targets is typically achieved through RT-qPCR or RNAseq. RT-qPCR is rapid but limited in number and complexity of targets detected, while RNAseq is high-throughput but takes multiple days.
Andrew T. Sullivan, Vibha Rao, Tyler Rockwood, Jahnavi Gandhi, Sarah Gruzka, Logan O’Connor, Bonnie Wang, Katherine B. Ragan, David Yu Zhang, Dmitriy Khodakov   +9 more
doaj   +1 more source

A hepatitis C virus cis-acting replication element forms a long-range RNA-RNA interaction with upstream RNA sequences in NS5B [PDF]

, 2008
The genome of hepatitis C virus (HCV) contains cis-acting replication elements (CREs) comprised of RNA stem-loop structures located in both the 5' and 3' non-coding regions (NCR), and in the NS5B coding sequence.
Diviney, Sinéad, Elliot, R., Evans, David John, Evans, D., Armstrong, V, Tuplin, Andrew, Evans, D.J., Struthers, Madeleine, Struthers, M., Elliott, Richard M., Diviney, S., Tuplin, A., Armstrong, V., Struthers, M, Simmonds, Peter, Elliott, Richard Michael, Armstrong, Victoria, Tuplin, A, Evans, David J., Simmonds, P., Diviney, S, Elliott, R.M., Simmonds, P   +22 more
core   +1 more source

Probe Generation Directly from Small Numbers of Cells for DNA Microarray Studies

BioTechniques, 2003
Recently, we described a technique that allows us to prepare probes for expression profiling from 0.5–1 μg RNA without template or signal amplification.
C.C. Xiang, M. Chen, O.A. Kozhich, Q.N. Phan, J.M. Inman, Y. Chen, M.J. Brownstein   +6 more
doaj   +1 more source

Development of a Chemiluminescence-Based Ribonuclease Protection Assay

BioTechniques, 2002
The ribonuclease protection assay (RPA) is a widely used method for the detection and quantification of specific mRNA transcripts in a complex mixture of total RNA or mRNA molecules. While exhibiting many advantages over other RNA detection methods, RPAs
Christopher Rosenau, Barbara Kaboord, M. Walid Qoronfleh   +2 more
doaj   +1 more source