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In ovo sexing and genotyping using PCR techniques: a contribution to the 3R principles in chicken breeding [PDF]

open access: yesScientific Reports
Early sex determination and genotyping of chicken embryos is crucial for ethical and resource-efficient animal research. We developed a reproducible workflow using whole genome amplification combined (WGA) with Kompetitive Allele Specific PCR (KASP) and ...
C. Dierks   +6 more
doaj   +2 more sources

Preventing chick culling in the poultry industry with a new biomarker for rapid in ovo gender screening [PDF]

open access: yesScientific Reports
Chicken eggs are one of the most consumed foods worldwide. However, the practice of chicken culling in the poultry industry involves unnecessary animal suffering and finding a way to put an end to this has become a societal priority.
Nicolas Drouin   +15 more
doaj   +2 more sources

Safety, Immunogenicity, and Vaccine Compatibility of a Trivalent Inactivated In Ovo Nanovaccine Against Avian Colibacillosis in Broilers Under Commercial Hatchery Conditions [PDF]

open access: yesAnimals
Avian colibacillosis, caused by Escherichia coli, remains a significant threat to poultry health and production, particularly in the context of rising antimicrobial resistance.
Angelo Scuotto   +8 more
doaj   +2 more sources

In ovo applications in poultry: A review

open access: yesPoultry Science, 2018
The various methods employed for the in ovo administration of different materials for promoting the health and productivity of poultry are discussed in this review article. The amnion has proven to be an effective site for injection and the timing of in ovo injection has commonly occurred at transfer.
exaly   +3 more sources

Quail development in in ovo and ex ovo conditions: Preincubation influences stress proteins and antioxidants

open access: yesVeterinary and Animal Science
Extreme temperatures, whether excessively high or low, are critical environmental stressors for organisms, profoundly impacting cellular metabolism and homeostasis.
Bronislava Pokorna   +4 more
doaj   +2 more sources

A Novel Breast Cancer Xenograft Model Using the Ostrich Chorioallantoic Membrane—A Proof of Concept

open access: yesVeterinary Sciences, 2023
The avian chorioallantoic membrane (CAM) assay has attracted scientific attention in cancer research as an alternative or complementary method for in vivo animal models. Here, we present a xenograft model based on the ostrich (struthio camelus) CAM assay
Marta Pomraenke   +7 more
doaj   +1 more source

Determination of Embryotoxic effects of Atipamezole using in ovo model

open access: yesRevista Científica, 2023
Atipamezole is a specific α2-adrenergic receptor antagonist, and there exists insufficient information on its use during pregnancy. The aim of this study was to determine the embryotoxic activity of Atipamezole through an in ovo method. During the first
Rahmi Canbar   +3 more
doaj   +1 more source

Effect of the In Ovo Injection Site of Electrolytes on Some Biochemical Blood Parameters and Quality of Layer Chicks

open access: yesAnimals, 2022
The effects of the in ovo injection site of electrolytes on selected biochemical blood parameters and the quality of layer chicks were investigated. A total of 120 fertile eggs from Rhode Island Red breeders were randomly distributed into 4 groups, with ...
Joanna Pawłowska   +2 more
doaj   +1 more source

In ovo administration of Bacillus subtilis serotypes effect hatchability, 21-day performance, and intestinal microflora

open access: yesPoultry Science, 2021
: Recent research has tried to maximize broiler chick health and performance by utilizing commercial in-feed probiotics to inoculate fertile hatching eggs, and thus expose birds earlier to beneficial bacteria.
Claudia D. Castañeda   +4 more
doaj   +1 more source

Chicken embryo as a model in epigenetic research

open access: yesPoultry Science, 2021
: Epigenetics is defined as the study of changes in gene function that are mitotically or meiotically heritable and do not lead to a change in DNA sequence.
Marek Bednarczyk   +3 more
doaj   +1 more source

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