<i>Pelecyphora chihuahuensis</i> (Britton & Rose) D. Aquino & Dan. Sánchez: A Review on Its Taxonomy, Ecology and Conservation of an Endemic Mexican Cactus Species with Biotechnological Perspectives. [PDF]
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In situ hybridization techniques involve specific annealing of labeled nucleic acid probes with complimentary cellular RNAs, and the subsequent detection of these labeled probes within fixed cells (1,2). In situ hybridization can be applied to monolayer cells, tissue sections, or whole mount tissues.
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In situ HiC uses the relative frequency of DNA-DNA ligation events to reconstruct the three-dimensional architecture of a genome. As such, restriction enzyme digested ends of genomic DNA within fixed nuclei are tagged with biotinylated dNTPs. DNA-DNA ligation events generated via proximity ligation are then captured, amplified and next generation ...
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