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Pelagic Microbial Communities in the North Sea: Cultivation, Diversity, and In Situ Dynamics
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Cultivation of sponges is being explored to supply biomaterial for the pharmaceutical and cosmetics industries. This study assesses the impact of various cultivation methods on the microbial community within the sponge Rhopaloeides odorabile during: (1) in situ cultivation under natural environmental conditions, (2) ex situ cultivation in small flow ...
Nicole S Webster +2 more
exaly +6 more sources
The identification and in situ cultivation of functional yet uncultivable microorganisms are important to confirm inferences regarding their ecological functions. Here, we developed a new method that couples Raman-activated cell sorting (RACS), stable-isotope probing (SIP), and genome-directed cultivation (GDC)─namely, RACS-SIP-GDC─to identify, sort ...
Jibing Li, Dayi Zhang, Chunling Luo
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In-situ determination of sediment oxygen demand in cultivation ponds
Chinese Journal of Oceanology and Limnology, 2000This paper presents a method for in-situ determining sediment oxygen demand (SOD) in cultivation pond. This method based on sediment surface structure, temperature, and other determining conditions like those in shrimp cultivation environments, overcomes defects of old methods and provides more accurate estimation of SOD's effect on dissolved oxygen in
Yao Sun
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In situ cultivation of previously uncultivable microorganisms using the ichip
Nature Protocols, 2017Most microbial species remain uncultivated, and modifying artificial nutrient media brings only an incremental increase in cultivability. We reasoned that an alternative way to cultivate species with unknown requirements is to use naturally occurring combinations of growth factors.
Brittany, Berdy +3 more
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On-line and in situ monitoring of biomass in submerged cultivations
Trends in Biotechnology, 1997Biomass is one of the most important variables in submerged-cultivation processes and, in recent years, many different sensors have been introduced for on-line or in situ monitoring of this variable. This article provides an overview of the principles behind these sensors and discusses their application in both laboratory and industrial processes.
Lisbeth Olsson, Jens Nielsen
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Online monitoring of microcarrier based fibroblast cultivations with in situ microscopy
Biotechnology and Bioengineering, 2007AbstractAnimal cell culture is widely used in biotechnology for the production of many biological products. In situ microscopes acquire images directly from cell suspensions and analyze the images in matters of cell concentration, cell size distribution and cell morphology.
Guido, Rudolph +6 more
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In-situ optimal control of nutrient solution for soilless cultivation
2011 3rd International Conference on Advanced Computer Control, 2011Soilless cultivation is one of main production modes in precision agriculture. Nutrient solution for soilless cultivation primarily consists of macroelements such as NO 3 −, H 2 PO 4 −, K+, Ca2+, Mg2+, and SO 4 2−. In vegetable-nutrient solution system, there are complicated correlations between greenhouse vegetable growth and nutrient solution, thus ...
null Feng Chen +2 more
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