Results 101 to 110 of about 2,744,329 (235)
MethodsX, 2019 A fluorescence in situ hybridization (FISH) protocol was developed for nematodes in which nucleic acid probes are introduced within the organism via electroporation.
Casey L. Ruark-Seward +2 more
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Single-molecule RNA Fluorescence in situ Hybridization (smFISH) in Caenorhabditis elegans
Bio-Protocol, 2017 Single-molecule RNA fluorescence in situ hybridization (smFISH) is a technique to visualize individual RNA molecules using multiple fluorescently-labeled oligonucleotide probes specific to the target RNA (Raj et al., 2008; Lee et al., 2016a).
ChangHwan Lee +5 more
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A stem-less probe using spontaneous pairing between Cy3 and quencher for RNA detection
Science and Technology of Advanced Materials, 2016 We herein report a stem-less probe for the detection of RNA that depends on pairing between Cy3 and nitro methyl red. In our design, two Cy3 residues and two nitro methyl red residues were introduced into an oligonucleotide. In the absence of the target,
Hiromu Kashida +2 more
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Acta Medica Bulgarica, 2017 Molecular biology of esophageal adenocarcinoma (EAC) is not fully elucidated. The aim of this study was to assess the expression of cycle regulator and tumor suppressor p16 in esophageal adenocarcinoma. The expression of p16 at protein and gene level was
Kotzev A., Kamenova M.
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Understanding the Impact of Synchronous, Asynchronous, and Hybrid In-Situ Techniques in Computational Fluid Dynamics Applications [PDF]
High-Performance Computing (HPC) systems provide input/output (IO) performance growing relatively slowly compared to peak computational performance and have limited storage capacity. Computational Fluid Dynamics (CFD) applications aiming to leverage the full power of Exascale HPC systems, such as the solver Nek5000, will generate massive data for ...
arxiv +1 more source
Therapeutics and Clinical Risk Management, 2017 Zhihe Liu,1 Siyun Li,2 Ou Bai1 1Department of Hematology, The First Hospital of Jilin University, Changchun, 2Department of Pediatrics, Women and Children’s hospital of Qingdao university, Qingdao, People’s Republic of China Abstract: We ...
Liu Z, Li S, Bai O
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Segmentation-free integration of nuclei morphology and spatial transcriptomics for retinal images [PDF]
arXivThis study introduces SEFI (SEgmentation-Free Integration), a novel method for integrating morphological features of cell nuclei with spatial transcriptomics data. Cell segmentation poses a significant challenge in the analysis of spatial transcriptomics data, as tissue-specific structural complexities and densely packed cells in certain regions make ...
arxiv
Fluorescent in situ hybridization has limitations in screening NRG1 gene rearrangements
Diagnostic PathologyBackground NRG1 fusion is a promising therapeutic target for various tumors but its prevalence is extremely low, and there are no standardized testing algorithms for genetic assessment. Mothods In this study, we analyzed 3008 tumors using Fluorescence in
Xiaomei Zhang +9 more
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Вавиловский журнал генетики и селекции, 2014 Presently, suppression of repetitive DNAsequences (chromosomal in situ suppression hybridization, СISS-hybridization) is used to improve the results of fluorescence in situ hybridization (FISH). However, in some cases the suppression cannot be performed,
A. G. Bogomolov +4 more
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Fluorescence in situ hybridization of genes in environmental microbiology [PDF]
, 2010 Our knowledge concerning the metabolic potentials of as yet uncultured microorganisms has increased tremendously with the advance of sequencing technologies and the consequent discovery of novel genes. On the other hand, it is often difficult to reliably
Moraru, Cristina Liliana
core +1 more source