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Fluorescence In Situ Hybridization
1999Within the past decade, the cytogenetics laboratory has witnessed a major advance in diagnostic and prognostic capability with the advent of molecular cytogenetics. The product of a combination of cytogenetics and molecular biology, the technique of fluorescence in situ hybridization (FISH), has increased the resolution and application of traditional ...
Gesa Schwanitz, Regine Schubert
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Fluorescence in situ hybridization
Blood Reviews, 1993Spectacular advances in the use of fluorescence in situ hybridization (FISH) for the visualisation of specific DNA sequences in metaphase chromosomes and interphase cells have been made over the last few years making the technique a useful tool in clinical research.
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Fluorescence In Situ Hybridization
Clinics in Laboratory Medicine, 2011This chapter presents past and present FISH techniques and specific applications of FISH. Although array technology has revolutionized cytogenetics, FISH remains indispensible. While array technology provides a high resolution screen of the entire genome for gains and losses, it does not allow for visualization of the genomic structure of gains.
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Fluorescence in situ hybridization: 1998
Luminescence, 1999The journal provides comprehensive literature searches on all aspects of luminescence which will be published in most issues. These lists have been produced regularly since 1986 and in excess of 5500 references have been cited—some by year and some by specialized topic.
Larry J. Kricka, P. E. Stanley
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An Introduction to Fluorescence in situ Hybridization in Microorganisms
2021Fluorescence in situ hybridization (FISH) is a molecular biology technique that enables the localization, quantification, and identification of microorganisms in a sample. This technique has found applications in several areas, most notably the environmental, for quantification and diversity assessment of microorganisms and, the clinical, for the rapid
Almeida, Carina, Azevedo, Nuno F.
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Fluorescent In Situ Hybridization
2008In situ hybridization (ISH) technique was introduced by Gall and Pardue1 in 1969. At that time the technique was limited by the use of radioactively labelled probes that were subsequently visualized by autoradiography. The development of interphase cytogenetics in the 1980s and fluorescent labels in 19862 has seen the technology applied in a number of ...
Fiona Campbell, John M. S. Bartlett
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Probe Labeling and Fluorescence In Situ Hybridization [PDF]
AbstractThis unit describes in detail basic protocols for probe labeling, denaturing of in situ target DNA, in situ hybridization, and post‐hybridization washes. Support protocols for probe labeling cover probe purification and quality control.
A.K. Raap, J. Wiegant
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Fluorescence In Situ Hybridization
2005Dr. Seuss’s eloquent “One FISH, two FISH, red FISH, blue FISH” (1) could have been describing one of the most significant advancements in clinical cytogenetics, fluorescence in situ hybridization (FISH). The process, as described by Pinkel et al. in 1988 (2), involved fluorescent detection of probe DNA hybridized to chromosomal target sequences.
Stuart Schwartz+2 more
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Fluorescence in situ hybridization: Uses and limitations
Seminars in Hematology, 2000The development of molecular hybridization techniques such as fluorescence in situ hybridization (FISH) has had a major Impact on efforts to detect and characterize the genetic changes that give rise to human tumors. With probes designed to Identify specific chromosomes and chromosomal regions, FISH is used routinely by cytogenetics and pathology ...
Gozzetti, A, Le Beau, M M
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Fluorescent In Situ Hybridization (FISH)
2021In situ hybridization (ISH) is a technique that uses nucleic acid probes to allow for visualization of nucleic acid sequences in cellular/tissue preparations. Both enzymatic and fluorescent ISH studies can be used as adjunct studies, and in some cases are vital to making specific diagnoses. This chapter will focus on fluorescent in situ hybridization.
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