Results 261 to 270 of about 234,009 (301)

Fluorescent In Situ Hybridization (FISH)

2021
In situ hybridization (ISH) is a technique that uses nucleic acid probes to allow for visualization of nucleic acid sequences in cellular/tissue preparations. Both enzymatic and fluorescent ISH studies can be used as adjunct studies, and in some cases are vital to making specific diagnoses. This chapter will focus on fluorescent in situ hybridization.
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An Introduction to Fluorescence in situ Hybridization in Microorganisms

2021
Fluorescence in situ hybridization (FISH) is a molecular biology technique that enables the localization, quantification, and identification of microorganisms in a sample. This technique has found applications in several areas, most notably the environmental, for quantification and diversity assessment of microorganisms and, the clinical, for the rapid
Almeida, Carina, Azevedo, Nuno F.
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Fluorescence in situ hybridization: Uses and limitations

Seminars in Hematology, 2000
The development of molecular hybridization techniques such as fluorescence in situ hybridization (FISH) has had a major Impact on efforts to detect and characterize the genetic changes that give rise to human tumors. With probes designed to Identify specific chromosomes and chromosomal regions, FISH is used routinely by cytogenetics and pathology ...
Gozzetti, A, Le Beau, M M
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Advances in fluorescence in situ hybridization

Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1998
The techniques of in situ hybridization (ISH) are widely applied for analyzing the genetic make-up and RNA expression patterns of individual cells. This review focusses on a number of advances made over the last 5 years in the fluorescence ISH (FISH) field, i.e., Fiber-FISH, Multi-colour chromosome painting, Comparative Genomic Hybridization, Tyramide ...
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Raman-Fluorescence in Situ Hybridization

2014
As with many other molecular microbiology methods, the family of stable isotope probing (SIP) techniques based on the analysis of isotope- labeled nucleic acids, phospholipid fatty acids (PLFAs), or proteins are frequently performed at the level of the community.
Read, Daniel, Whiteley, Andrew
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Fluorescence in Situ Hybridization

1999
In situ hybridization (ISH) combines cytogenetic and molecular genetic techniques. The principle of ISH is the interaction of a labelled singlestranded DNA or RNA probe with a complementary single stranded target DNA sequence. The presence of the target DNA is proven in metaphase chromosomes or in interphase nuclei which are fixed on slides hence in ...
Gesa Schwanitz, Regine Schubert
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Fluorescence In Situ Hybridization in Oat

2017
This chapter describes methods to detect gene loci or gene transcripts by fluorescence labeling. Fluorescence in situ hybridization (FISH) can be used to identify the positions of genes or BACs or the distribution of repetitive sequences on metaphase chromosomes as well as the identification of alien chromosomes.
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Fluorescent In Situ Hybridization

2001
Fluorescence in situ hybridization (FISH) arose from a marriage of classical DNA hybridization in solution to modern molecular biologic techniques, most notably the use of restriction endonucleases and, later, the polymerase chain reaction (PCR). The former has made identification of both genes and relevant interspersed sequences possible, whereas ...
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Fluorescence In situ Hybridization

1996
During the last decade, pathology has progressed remarkably with the incorporation of molecular techniques into the arena of diagnostics. Fluorescence in situ hybridization (FISH) is a molecular histopathological technique that can be readily used to identify DNA or RNA abnormalities at the cellular level with the use of an epifluorescence microscope ...
Sunny Luke, Victoria Belogolovkin, Jerry A. Varkey, Charles T. Ladoulis   +3 more
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Fluorescence In Situ Hybridization

2017
Fluorescence in situ hybridization (FISH) is a molecular cytogenetic technique used to physically map DNA sequences on chromosomes. In FISH, DNA probes are most often hybridized to target DNA in metaphase chromosomes spread on slides (in situ). FISH probes are variable in size.
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