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Fluorescence In Situ Hybridization
2017Fluorescence in situ hybridization (FISH) is a molecular cytogenetic technique used to physically map DNA sequences on chromosomes. In FISH, DNA probes are most often hybridized to target DNA in metaphase chromosomes spread on slides (in situ). FISH probes are variable in size.
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Fluorescent In Situ Hybridization
2001Fluorescence in situ hybridization (FISH) arose from a marriage of classical DNA hybridization in solution to modern molecular biologic techniques, most notably the use of restriction endonucleases and, later, the polymerase chain reaction (PCR). The former has made identification of both genes and relevant interspersed sequences possible, whereas ...
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Fluorescence In Situ Hybridization and Comparative Genomic Hybridization
2006This chapter discusses the complementary methodologies of fluorescence in situ hybridization and comparative genomic hybridization. Fluorescence in situ hybridization uses fluorescently labeled DNA probes (whole chromosomes, centromere, or locus-specific sequences) to visualize complementary DNA sequences in the target DNA (metaphase chromosomes or ...
Patricia, Gorman, Rebecca, Roylance
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Fluorescence in situ hybridization
1997Abstract In situ hybridization enables the visualization of a probe of interest within a cytological context. For the purposes of gene mapping, the probe is usually a particular segment of cloned DNA which is labelled to allow detection. The target is normally a preparation of chromosomal DNA on a microscope slide.
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Fluorescence in situ hybridization
2007Princip hibridizacijskih tehnika in situ počiva na komplementarnom vezanju obilježene sonde sa specifičnim odsječkom DNA na citogenetskom preparatu, što omogućava izravno utvrđivanje i lokalizaciju određenih sljedova DNA na kromosomu. Stoga ove metode imaju mnoge primjene u istraživanju i dijagnostici (npr.
Mravinac, Brankica +1 more
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Cancer statistics: Breast cancer in situ
Ca-A Cancer Journal for Clinicians, 2015Elizabeth M Ward +2 more
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