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Standard Fluorescence in situ Hybridization Procedures [PDF]
Fluorescence in situ hybridization (FISH) allows nucleic acid sequences to be visualized inside fi xed cells or chromosomes. The method, based on the principle that nucleic acids can denature and renature, allows the detection of a known nucleotide sequence (probe), in a cell/chromosome, thanks to its ability to hybridize the complementary nucleic-acid
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Fluorescence in situ Hybridization
2017Fluorescence in situ hybridization (FISH) is a molecular cytogenetic technique used to physically map DNA sequences on chromosomes. In FISH, DNA probes are most often hybridized to target DNA in metaphase chromosomes spread on slides (in situ). FISH probes are variable in size.
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Fluorescence in situ hybridization
1997Abstract In situ hybridization enables the visualization of a probe of interest within a cytological context. For the purposes of gene mapping, the probe is usually a particular segment of cloned DNA which is labelled to allow detection. The target is normally a preparation of chromosomal DNA on a microscope slide.
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Five-color fluorescence in situ hybridization system for karyotyping of Panax ginseng
Horticulture Environment and Biotechnology, 2020N. Waminal +3 more
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Fundamentals and developments in fluorescence-guided cancer surgery
Nature Reviews Clinical Oncology, 2021Friso Achterberg +2 more
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Detection of Microorganisms by Fluorescence In Situ Hybridization Using Peptide Nucleic Acid.
Methods in molecular biology, 2020Ricardo Oliveira, C. Almeida, N. Azevedo
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