A modified protocol for the detection of three different mRNAs with a new-generation in situ hybridization chain reaction on frozen sections [PDF]
A new multiple fluorescence in situ hybridization method based on hybridization chain reaction was recently reported, enabling simultaneous mapping of multiple target mRNAs within intact zebrafish and mouse embryos.
Burnstock, G+7 more
core
Rapid micro fluorescence in situ hybridization in tissue sections [PDF]
This paper describes a micro fluorescence in situ hybridization ({\mu}FISH)-based rapid detection of cytogenetic biomarkers on formalin-fixed paraffin embedded (FFPE) tissue sections. We demonstrated this method in the context of detecting human epidermal growth factor 2 (HER2) in breast tissue sections.
arxiv +1 more source
In patients with low and intermediate risk myelodysplastic syndrome and deletion 5q (del(5q)) treated with lenalidomide, monitoring of cytogenetic response is mandatory, since patients without cytogenetic response have a significantly increased risk of ...
Gudrun Göhring+7 more
doaj +1 more source
Rapid interphase and metaphase assessment of specific chromosomal changes in neuroectodermal tumor cells by in situ hybridization with chemically modified DNA probes [PDF]
Repeated DNAs from the constitutive heterochromatin of human chromosomes 1 and 18 were used as probes in nonradioactive in situ hybridization experiments to define specific numerical and structural chromosome aberrations in three human glioma cell lines ...
A.H.N. Hopman+64 more
core +1 more source
The origin of human chromosome 2 analyzed by comparative chromosome mapping with a DNA microlibrary [PDF]
Fluorescencein situ hybridization (FISH) of microlibraries established from distinct chromosome subregions can test the evolutionary conservation of chromosome bands as well as chromosomal rearrangements that occurred during primate evolution and will ...
A Baldini+32 more
core +1 more source
Advances and Challenges in Fluorescence in situ Hybridization for Visualizing Fungal Endobacteria
Several bacteria have long been known to interact intimately with fungi, but molecular approaches have only recently uncovered how cosmopolitan these interactions are in nature.
Demosthenes P. Morales+8 more
doaj +1 more source
Drawback of chimerism analysis by XY-fluorescence in situ hybridization: Deception of a relapse
Chimerism is monitored by various methods in posttransplant patients. Interphase fluorescence in situ hybridization (FISH) for XY (sex) chromosomes is one of the methods commonly employed.
D VS Kiran Kumar+1 more
doaj +1 more source
Background Triple-negative breast carcinoma (TNBC) patients do not benefit from hormone- or human epidermal growth factor receptor 2- (HER2-) targeted therapies. Accurate testing is pivotal for these patients. Methods TNBC cases that were retested at our
Jose De Jesus, Marilin Rosa
doaj +1 more source
Multicolor fluorescence in situ hybridization on metaphase chromosomes and interphase Halo-preparations using cosmid and YAC clones for the simultaneous high resolution mapping of deletions in the dystrophin gene [PDF]
We report on multicolor fluorescence in situ hybridization protocols for the simultaneous visualization of deletion-prone regions for carrier detection of Duchenne/ Becker (DMD/BMD) muscular dystrophy.
Blonden, Lau+6 more
core +1 more source
Multimodal registration of FISH and nanoSIMS images using convolutional neural network models [PDF]
Nanoscale secondary ion mass spectrometry (nanoSIMS) and fluorescence in situ hybridization (FISH) microscopy provide high-resolution, multimodal image representations of the identity and cell activity respectively of targeted microbial communities in microbiological research.
arxiv