Testing the Potential Clastogenic/Cytotoxic Effects of Pesticide CALYPSO 480 SC
The detection of chromosomal damage serves as a tool for the verification of the genotoxic effects of chemical substances in vitro. We used conventional cytogenetic analysis in order to test for the potential genotoxic action of the insecticide ...
Ficová I., Galdíková M.
doaj +1 more source
Genome composition of 'Elatior'-begonias hybrids analyzed by genomic in situ hybridisation [PDF]
Interspecific hybridization of various tuberous Begonia species hybrids with Begonia socotrana results in so-called 'Elatior'-begonias hybrids (B. x hiemalis Fotsch).
Laak, W.A.+3 more
core +2 more sources
FISHing in Uncertainty: Synthetic Contrastive Learning for Genetic Aberration Detection [PDF]
Detecting genetic aberrations is crucial in cancer diagnosis, typically through fluorescence in situ hybridization (FISH). However, existing FISH image classification methods face challenges due to signal variability, the need for costly manual annotations and fail to adequately address the intrinsic uncertainty.
arxiv +1 more source
Problems In Determining Her2 Status In Breast Carcinoma
Objective:Human epidermal growth factor receptor 2 (HER2) oncoprotein is overexpressed in 15-25% of breast carcinomas and associated with poor outcome.
Emel Ebru Pala+5 more
doaj +1 more source
Sorting of chromosomes by magnetic separation [PDF]
Chromosomes were isolated from Chinese hamster x human hybrid cell lines containing four and nine human chromosomes. Human genomic DNA was biotinylated by nick translation and used to label the human chromosomes by in situ hybridization in suspension ...
A Oberteufer+30 more
core +1 more source
Modeling Intratumor Gene Copy Number Heterogeneity using Fluorescence in Situ Hybridization data [PDF]
Tumorigenesis is an evolutionary process which involves a significant number of genomic rearrangements typically coupled with changes in the gene copy number profiles of numerous cells. Fluorescence in situ hybridization (FISH) is a cytogenetic technique which allows counting copy numbers of genes in single cells.
arxiv
A Hybrid Reconstruction Approach for Absorption Coefficient by Fluorescence Photoacoustic Tomography [PDF]
In this paper, we propose a hybrid method to reconstruct the absorption coefficient by fluorescence photoacoustic tomography (FPAT), which combines a squeeze iterative method (SIM) and a nonlinear optimization method. The SIM is to use two monotonic sequences to squeeze the exact coefficient, and it quickly locates near the exact coefficient.
arxiv +1 more source
Detection of amplified DNA sequences by reverse chromosome painting using genomic tumor DNA as probe [PDF]
A modification of reverse chromosome painting was carried out using genomic DNA from tumor cells as a complex probe for chromosomal in situ suppression hybridization to normal metaphase chromsome spreads.
Cremer, Thomas+5 more
core +1 more source
Fluorescence In Situ Hybridization Method for Measuring Transfection Efficiency
We describe here the use of fluorescence in situ hybridization (FISH) to measure the transfection efficiency of the transient expression vector pCMVcat in lymphoblasts and fibroblasts.
Lie Cheng, Corazon D. Bucana, Qingyi Wei
doaj +1 more source
Fluorescence in situ hybridization (FISH) Protocol in Human Sperm [PDF]
Aneuploidies are the most frequent chromosomal abnormalities in humans. Most of these abnormalities result from meiotic errors during the gametogenic process in the parents.
Anton, Ester, Sarrate, Zaida
core +2 more sources